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The map label for this gene is citA [H]

Identifier: 50083651

GI number: 50083651

Start: 388255

End: 389574

Strand: Reverse

Name: citA [H]

Synonym: ACIAD0395

Alternate gene names: 50083651

Gene position: 389574-388255 (Counterclockwise)

Preceding gene: 50083652

Following gene: 50083645

Centisome position: 10.83

GC content: 42.8

Gene sequence:

>1320_bases
GTGGAACATGGACAACAAACATCCCGTATACGTGCCATTTTTAATGTAACAAGTGGCAACTTCTTAGAACAGTACGATTT
CTTCTTATTTGGTCTATATGCCAAAGCGATTGGCGAAACTTTTTTCCATTCTGATAGCAGTTATGCTGCACTCATGAAAA
CCTTTCTGGTTTTTGCAGTCAGCTTTTTGATGCGTCCAATTGGCGCATTGGTGCTGGGGCCTTATGTGGATCGGATCGGA
CGACGTAAAGGTTTGATGGTCACCTTGAGTATTATGGCGGTTGGCTCTTTAATTATTGCTTTAACCCCAGGCTACGCAAC
CATTGGTTTTGCTGCCACTGTTCTGATTTTTGCTGGTCGTCTTGCACAAGGTTTTTCCGCAGGTGTAGAACTTGGCGGTG
TTTCGGTGTATTTGTCCGAAATTGCCGAGCCTCATAATCGTGGTTTTATTACCAGTTGGCAATCTGCGAGTCAGCAAGTC
GCCGTTATTTTTGCAGCAACGCTCGGTTATATGATTAGCCTGGTCTTTACCAAAGCTCAAGTGGCTGATTGGGCTTGGCG
CATTCCGTTTTTTGTTGGCTGTGCCATCATCCCATTTATTTTCTGGTTGCGTAAATCTCTGCAAGAAACAGAAGCGTTTG
CCTCAAGAAAAGAACATCCAACGACTCAACAGATCATGTCAACACTGGCAGCGAACTGGAAAATTGTATTGACTGGCATG
TGTATGGTTGCAACCACGACCACCATGTTCTATTTCATTACAGTTTATACGCCAACCTATGGTAAGGAAGTTTTGCATTT
AACCAGTGCAGAAAGCTTACTGGCAACCGTGATGGTAGGCGTAAGTAATTTTATATTACTTCCAATTGGTGGTTATCTTT
CTGATAAATTTGGTCGCAAACCTTTATTGATTACAACGACCACGCTGATCTTTATTACGGCTTATCCACTTCTCAGTTGG
CTGACCCAGAATATTAGCCTGACTCATATGCTGATATCTTTGCTTTGGTTATCATTACTTTACAGTTTTTATAATGGTGC
GCTGGTCGTATCTTTAACTGAAATGATGCCAAGCTCGGTACGAATTGCTGGATTTTCAGTCGCCTATAGTTTGGCAACCT
CGGTATTTGGTGGTTTAACTCCAATGATTGCAACCTATCTTGTAGAGCAAAGTGGTAGCAAAAGCATGCCAGCTTTCTGG
CTGATGTTGGCAGCCATCATTAGTTTGATCGCGACATTGATTGTATTTCGCAAACGTCAGCACAATACGGCTCAATCCAA
GCCCCTTCAAACAAAATTAGCCTCTTCAGTATCTCACTGA

Upstream 100 bases:

>100_bases
TTTTATTTTAGTTTATGATTCAATTAATTTTTTAAGTTTATTTAAAGATTGTCTATTTATTCTCCACACTACGATGACAG
ACAAATGATGGAGAGTCGTT

Downstream 100 bases:

>100_bases
TCATGCTGTGACTCTGTTTCGGTATCTCTTGATCCGAATCAGAGTCATTCTGTTCTATATTGATTTATTTAGATTTCAAC
TGTTTCAGAATCGCTTTCAA

Product: citrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MEHGQQTSRIRAIFNVTSGNFLEQYDFFLFGLYAKAIGETFFHSDSSYAALMKTFLVFAVSFLMRPIGALVLGPYVDRIG
RRKGLMVTLSIMAVGSLIIALTPGYATIGFAATVLIFAGRLAQGFSAGVELGGVSVYLSEIAEPHNRGFITSWQSASQQV
AVIFAATLGYMISLVFTKAQVADWAWRIPFFVGCAIIPFIFWLRKSLQETEAFASRKEHPTTQQIMSTLAANWKIVLTGM
CMVATTTTMFYFITVYTPTYGKEVLHLTSAESLLATVMVGVSNFILLPIGGYLSDKFGRKPLLITTTTLIFITAYPLLSW
LTQNISLTHMLISLLWLSLLYSFYNGALVVSLTEMMPSSVRIAGFSVAYSLATSVFGGLTPMIATYLVEQSGSKSMPAFW
LMLAAIISLIATLIVFRKRQHNTAQSKPLQTKLASSVSH

Sequences:

>Translated_439_residues
MEHGQQTSRIRAIFNVTSGNFLEQYDFFLFGLYAKAIGETFFHSDSSYAALMKTFLVFAVSFLMRPIGALVLGPYVDRIG
RRKGLMVTLSIMAVGSLIIALTPGYATIGFAATVLIFAGRLAQGFSAGVELGGVSVYLSEIAEPHNRGFITSWQSASQQV
AVIFAATLGYMISLVFTKAQVADWAWRIPFFVGCAIIPFIFWLRKSLQETEAFASRKEHPTTQQIMSTLAANWKIVLTGM
CMVATTTTMFYFITVYTPTYGKEVLHLTSAESLLATVMVGVSNFILLPIGGYLSDKFGRKPLLITTTTLIFITAYPLLSW
LTQNISLTHMLISLLWLSLLYSFYNGALVVSLTEMMPSSVRIAGFSVAYSLATSVFGGLTPMIATYLVEQSGSKSMPAFW
LMLAAIISLIATLIVFRKRQHNTAQSKPLQTKLASSVSH
>Mature_439_residues
MEHGQQTSRIRAIFNVTSGNFLEQYDFFLFGLYAKAIGETFFHSDSSYAALMKTFLVFAVSFLMRPIGALVLGPYVDRIG
RRKGLMVTLSIMAVGSLIIALTPGYATIGFAATVLIFAGRLAQGFSAGVELGGVSVYLSEIAEPHNRGFITSWQSASQQV
AVIFAATLGYMISLVFTKAQVADWAWRIPFFVGCAIIPFIFWLRKSLQETEAFASRKEHPTTQQIMSTLAANWKIVLTGM
CMVATTTTMFYFITVYTPTYGKEVLHLTSAESLLATVMVGVSNFILLPIGGYLSDKFGRKPLLITTTTLIFITAYPLLSW
LTQNISLTHMLISLLWLSLLYSFYNGALVVSLTEMMPSSVRIAGFSVAYSLATSVFGGLTPMIATYLVEQSGSKSMPAFW
LMLAAIISLIATLIVFRKRQHNTAQSKPLQTKLASSVSH

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=411, Percent_Identity=30.6569343065693, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1788942, Length=419, Percent_Identity=31.980906921241, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1788292, Length=415, Percent_Identity=27.710843373494, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789941, Length=437, Percent_Identity=28.3752860411899, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87082231, Length=236, Percent_Identity=27.1186440677966, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48225; Mature: 48225

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHGQQTSRIRAIFNVTSGNFLEQYDFFLFGLYAKAIGETFFHSDSSYAALMKTFLVFAV
CCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SFLMRPIGALVLGPYVDRIGRRKGLMVTLSIMAVGSLIIALTPGYATIGFAATVLIFAGR
HHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHH
LAQGFSAGVELGGVSVYLSEIAEPHNRGFITSWQSASQQVAVIFAATLGYMISLVFTKAQ
HHHHHHCCCHHCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VADWAWRIPFFVGCAIIPFIFWLRKSLQETEAFASRKEHPTTQQIMSTLAANWKIVLTGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEHH
CMVATTTTMFYFITVYTPTYGKEVLHLTSAESLLATVMVGVSNFILLPIGGYLSDKFGRK
HHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCC
PLLITTTTLIFITAYPLLSWLTQNISLTHMLISLLWLSLLYSFYNGALVVSLTEMMPSSV
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCE
RIAGFSVAYSLATSVFGGLTPMIATYLVEQSGSKSMPAFWLMLAAIISLIATLIVFRKRQ
EEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
HNTAQSKPLQTKLASSVSH
CCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEHGQQTSRIRAIFNVTSGNFLEQYDFFLFGLYAKAIGETFFHSDSSYAALMKTFLVFAV
CCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SFLMRPIGALVLGPYVDRIGRRKGLMVTLSIMAVGSLIIALTPGYATIGFAATVLIFAGR
HHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHH
LAQGFSAGVELGGVSVYLSEIAEPHNRGFITSWQSASQQVAVIFAATLGYMISLVFTKAQ
HHHHHHCCCHHCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VADWAWRIPFFVGCAIIPFIFWLRKSLQETEAFASRKEHPTTQQIMSTLAANWKIVLTGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEHH
CMVATTTTMFYFITVYTPTYGKEVLHLTSAESLLATVMVGVSNFILLPIGGYLSDKFGRK
HHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCC
PLLITTTTLIFITAYPLLSWLTQNISLTHMLISLLWLSLLYSFYNGALVVSLTEMMPSSV
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCE
RIAGFSVAYSLATSVFGGLTPMIATYLVEQSGSKSMPAFWLMLAAIISLIATLIVFRKRQ
EEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
HNTAQSKPLQTKLASSVSH
CCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]