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The map label for this gene is hisIE [H]

Identifier: 50083639

GI number: 50083639

Start: 373492

End: 374265

Strand: Reverse

Name: hisIE [H]

Synonym: ACIAD0380

Alternate gene names: 50083639

Gene position: 374265-373492 (Counterclockwise)

Preceding gene: 50083640

Following gene: 50083638

Centisome position: 10.4

GC content: 42.38

Gene sequence:

>774_bases
ATGAACAACGTGCAATGGCTCGATGAAGTAAAATTTAACGAACAAGGATTAATCCCGGCCATCGCTCAGCATCATCAAAC
TGGACGTATATTAATGGTCGCGTGGATGAATCGTGAATCGCTGGCACTTACAGCCGAAAAGAAAGAGGCTGTATATTATT
CACGTTCACGCCAAAAATTATGGCATAAAGGTGAAGAATCAGGCCACTTTCAGACCGTTTATGAAATTCGTCTGGATTGC
GATGGCGATGTGATTATTTTACAGATTGAACAACATGGCGGCATCGCCTGCCATACTGGCCGTGAATCTTGTTTTTATCG
TAAGCTTACTCCGCAAGGTTGGGAGATTGTCGATGCGCAGTTAAAAGACCCTACTGCGATTTATGGTCAATCGGCTAAGG
TTGAAGCGCATGATCATGGTCATTCAAACGAACAGGTGGATGTATTATCATATCTCGGTCAATTAATGCAGCAACGTAAG
CAAGCAACAGCCGACAGCTCATATATCGCCAGCCTTTATCAAAAAGGACTCAATAAGATTCTTGAAAAAGTTGGCGAGGA
AAATATTGAAACGATTATTGCAGCCAAAGATTATGCTGCCGAACCGACTCAAGAACATTTAAATGATTTAATCTATGAAA
CTGCCGATATGTGGTTTCATAGTATTGTCATGCTCGGCTATTTCGATCTTACGCCACAAGCCGTTTTGGATGAGCTTGCT
CGCCGTCAGGGTTTATCAGGTCTTGTTGAAAAAGCCAATCGCAATCAAGGATAA

Upstream 100 bases:

>100_bases
TTTTTTTCGCAAATCTGGTGAGATGCCGTACACTATATAGCTGTCTTTCTAGGCAGTCCGATCAACCTTTTTATATTTCC
TTTAATTTTGGTGATGTCTC

Downstream 100 bases:

>100_bases
AAATTACCTGTGTCGAGTATAGAAAAACCGAAGGCAACCTATGAACAAGCAACCGCAATTGATAATGCACGTCTAGGTGA
ATCCTTTAAGGTCATTGCCT

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase

Products: NA

Alternate protein names: PRA-CH [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNNVQWLDEVKFNEQGLIPAIAQHHQTGRILMVAWMNRESLALTAEKKEAVYYSRSRQKLWHKGEESGHFQTVYEIRLDC
DGDVIILQIEQHGGIACHTGRESCFYRKLTPQGWEIVDAQLKDPTAIYGQSAKVEAHDHGHSNEQVDVLSYLGQLMQQRK
QATADSSYIASLYQKGLNKILEKVGEENIETIIAAKDYAAEPTQEHLNDLIYETADMWFHSIVMLGYFDLTPQAVLDELA
RRQGLSGLVEKANRNQG

Sequences:

>Translated_257_residues
MNNVQWLDEVKFNEQGLIPAIAQHHQTGRILMVAWMNRESLALTAEKKEAVYYSRSRQKLWHKGEESGHFQTVYEIRLDC
DGDVIILQIEQHGGIACHTGRESCFYRKLTPQGWEIVDAQLKDPTAIYGQSAKVEAHDHGHSNEQVDVLSYLGQLMQQRK
QATADSSYIASLYQKGLNKILEKVGEENIETIIAAKDYAAEPTQEHLNDLIYETADMWFHSIVMLGYFDLTPQAVLDELA
RRQGLSGLVEKANRNQG
>Mature_257_residues
MNNVQWLDEVKFNEQGLIPAIAQHHQTGRILMVAWMNRESLALTAEKKEAVYYSRSRQKLWHKGEESGHFQTVYEIRLDC
DGDVIILQIEQHGGIACHTGRESCFYRKLTPQGWEIVDAQLKDPTAIYGQSAKVEAHDHGHSNEQVDVLSYLGQLMQQRK
QATADSSYIASLYQKGLNKILEKVGEENIETIIAAKDYAAEPTQEHLNDLIYETADMWFHSIVMLGYFDLTPQAVLDELA
RRQGLSGLVEKANRNQG

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=227, Percent_Identity=33.0396475770925, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319819, Length=77, Percent_Identity=38.961038961039, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH [H]

EC number: =3.5.4.19 [H]

Molecular weight: Translated: 29229; Mature: 29229

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVQWLDEVKFNEQGLIPAIAQHHQTGRILMVAWMNRESLALTAEKKEAVYYSRSRQKL
CCCCCCHHHCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHH
WHKGEESGHFQTVYEIRLDCDGDVIILQIEQHGGIACHTGRESCFYRKLTPQGWEIVDAQ
HHCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCEEEECCCCHHEEECCCCCCCEEEECC
LKDPTAIYGQSAKVEAHDHGHSNEQVDVLSYLGQLMQQRKQATADSSYIASLYQKGLNKI
CCCCHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LEKVGEENIETIIAAKDYAAEPTQEHLNDLIYETADMWFHSIVMLGYFDLTPQAVLDELA
HHHHCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RRQGLSGLVEKANRNQG
HHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MNNVQWLDEVKFNEQGLIPAIAQHHQTGRILMVAWMNRESLALTAEKKEAVYYSRSRQKL
CCCCCCHHHCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHH
WHKGEESGHFQTVYEIRLDCDGDVIILQIEQHGGIACHTGRESCFYRKLTPQGWEIVDAQ
HHCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCEEEECCCCHHEEECCCCCCCEEEECC
LKDPTAIYGQSAKVEAHDHGHSNEQVDVLSYLGQLMQQRKQATADSSYIASLYQKGLNKI
CCCCHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LEKVGEENIETIIAAKDYAAEPTQEHLNDLIYETADMWFHSIVMLGYFDLTPQAVLDELA
HHHHCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RRQGLSGLVEKANRNQG
HHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]