Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 50083628

GI number: 50083628

Start: 359381

End: 360865

Strand: Direct

Name: nusA [H]

Synonym: ACIAD0368

Alternate gene names: 50083628

Gene position: 359381-360865 (Clockwise)

Preceding gene: 50083627

Following gene: 50083629

Centisome position: 9.99

GC content: 43.5

Gene sequence:

>1485_bases
ATGAGCCGTGAAATTCTTACCGTTGTAGAAACGGTTAGTAATGAAAAAGGTGTTAGTCGTGAAGCGATCTTCGAAGCGCT
GGAACAAGCTCTTGTTGCGGCAACGAAGAAAAAATTCTACGAAGGCACAAATGCAGAAGAAGCACAATTACGTGTAGAAA
TTGACCGCAAAACAGGTGATTACCGCACGTTCCGTCAGTGGACACTTGTTGCGGATGAAGATCATGAAATGCCAGCATGC
CAAGACGCGATTTCTGATGTTGATGCATCAAAATGGTCAATTGGTGATGTGCGTGAGCTTGAAGTAGAGTCGATCGAATT
TGGTCGTATTGCAGCACAAATTGCCAAGCAAGTGATTGTACAGAAAATTCGTGAAGCAGAACGCGCATTAATTGCCGATG
CTTACGAATCAAAAGTGGGTGAGCTGATTTACGGTGAAGTGAAAAAGCAAACCAAAGATGGTTTCATCATTGATCTTGGT
GACAATGCGGAAGCGTATCTGGCACGTGAAGAGATGATTGCAAAAGAAATTTTGCGTCCAAAACAACGTATGAATGCAAT
TTTATACAGTGTCAATCGTGAAGGTCGTGGTGCGCAATTGTTATTGTCGCGCGCGCGTCCTGAAATGTTAATGGCATTGA
TGAAGAAAGAAATTCCAGAAATTTCTGAAGAAATCATTGAGATCAAGGCTGCTGCGCGTCAACCAGGTGTGCGTGCCAAA
ATTGCGGTGAAAACCAACGATCATCGTATCGATCCAGTCGGTGCGTGTATTGGTATGCGTGGTACACGTATTCAAGCAGT
TCAGCAAGAACTCAATGGTGAGCGTATTGATGTGGTGGTGTGGTCAGATGATCCAGCACAGTATATCGCAAGCGCACTTG
AACCTGCGGATGTGTCGGGAATTGTACTTGATGAAGATACACGCAGTGCTGACATTATTTTTGCTACCAATGATCAGCTA
GCTCGCGCTATTGGATCACAAGGTCAAAATGTTCGTCTTGCTTCAGAATTAACTGGCTATAAACTCGACATGATGCTTGA
AGAAGAGTATCGTGCTCGTCAGCAAAATGAAGCGCAGCAATATCTGGATATGTTTGTAACACGTCTGGATATTGAAGAAG
ATCTCGCAATGGCACTGGTTGAAATGGGCTTTACTTCACTTGAAGAAATTGCTTATGTGCCACCAGAAACATTTGACGAA
ATCGAACTTGATCAGGAACTGGTTGAGTTATTGCAAAGTCGTGCAAAAGAAGCAGCATTAACAGATGCATTAAAACAGCA
AGAAAATGTTCAGGAACCAAGTGTTGAGCTCCTTGCAATGGCAGGAATGACACAAGAACTGGCATATGCTCTTGCAGCAC
GTGGTGTCGTGACAGTTGATGACTTGGCTGATCAGGCTACTGATGATATTTCTGATATCGAAGGGCTTAGTGAAGAGAAA
GCGGGTCAACTGATCATGAAAGCACGCGAATCATGGTTTAATTAG

Upstream 100 bases:

>100_bases
GTAAGCATGTGCTTGAGATTGATAGTCATAATATCGACAAAGCAAACTTGATCTATCAGGACTAAATTCATTTAAATTAA
TAAGAATCTGTAGGTGACTT

Downstream 100 bases:

>100_bases
GAGGTAATACATGACGGACAAGTCGATTAAAGAGTTAGCACTCAGTGTAGGTCGTCCTGTAGAAAAGCTCCTTGAACAGG
TTCGTGAAGCAGGTCTGCCA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSREILTVVETVSNEKGVSREAIFEALEQALVAATKKKFYEGTNAEEAQLRVEIDRKTGDYRTFRQWTLVADEDHEMPAC
QDAISDVDASKWSIGDVRELEVESIEFGRIAAQIAKQVIVQKIREAERALIADAYESKVGELIYGEVKKQTKDGFIIDLG
DNAEAYLAREEMIAKEILRPKQRMNAILYSVNREGRGAQLLLSRARPEMLMALMKKEIPEISEEIIEIKAAARQPGVRAK
IAVKTNDHRIDPVGACIGMRGTRIQAVQQELNGERIDVVVWSDDPAQYIASALEPADVSGIVLDEDTRSADIIFATNDQL
ARAIGSQGQNVRLASELTGYKLDMMLEEEYRARQQNEAQQYLDMFVTRLDIEEDLAMALVEMGFTSLEEIAYVPPETFDE
IELDQELVELLQSRAKEAALTDALKQQENVQEPSVELLAMAGMTQELAYALAARGVVTVDDLADQATDDISDIEGLSEEK
AGQLIMKARESWFN

Sequences:

>Translated_494_residues
MSREILTVVETVSNEKGVSREAIFEALEQALVAATKKKFYEGTNAEEAQLRVEIDRKTGDYRTFRQWTLVADEDHEMPAC
QDAISDVDASKWSIGDVRELEVESIEFGRIAAQIAKQVIVQKIREAERALIADAYESKVGELIYGEVKKQTKDGFIIDLG
DNAEAYLAREEMIAKEILRPKQRMNAILYSVNREGRGAQLLLSRARPEMLMALMKKEIPEISEEIIEIKAAARQPGVRAK
IAVKTNDHRIDPVGACIGMRGTRIQAVQQELNGERIDVVVWSDDPAQYIASALEPADVSGIVLDEDTRSADIIFATNDQL
ARAIGSQGQNVRLASELTGYKLDMMLEEEYRARQQNEAQQYLDMFVTRLDIEEDLAMALVEMGFTSLEEIAYVPPETFDE
IELDQELVELLQSRAKEAALTDALKQQENVQEPSVELLAMAGMTQELAYALAARGVVTVDDLADQATDDISDIEGLSEEK
AGQLIMKARESWFN
>Mature_493_residues
SREILTVVETVSNEKGVSREAIFEALEQALVAATKKKFYEGTNAEEAQLRVEIDRKTGDYRTFRQWTLVADEDHEMPACQ
DAISDVDASKWSIGDVRELEVESIEFGRIAAQIAKQVIVQKIREAERALIADAYESKVGELIYGEVKKQTKDGFIIDLGD
NAEAYLAREEMIAKEILRPKQRMNAILYSVNREGRGAQLLLSRARPEMLMALMKKEIPEISEEIIEIKAAARQPGVRAKI
AVKTNDHRIDPVGACIGMRGTRIQAVQQELNGERIDVVVWSDDPAQYIASALEPADVSGIVLDEDTRSADIIFATNDQLA
RAIGSQGQNVRLASELTGYKLDMMLEEEYRARQQNEAQQYLDMFVTRLDIEEDLAMALVEMGFTSLEEIAYVPPETFDEI
ELDQELVELLQSRAKEAALTDALKQQENVQEPSVELLAMAGMTQELAYALAARGVVTVDDLADQATDDISDIEGLSEEKA
GQLIMKARESWFN

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=497, Percent_Identity=52.7162977867203, Blast_Score=503, Evalue=1e-143,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55227; Mature: 55096

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREILTVVETVSNEKGVSREAIFEALEQALVAATKKKFYEGTNAEEAQLRVEIDRKTGD
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
YRTFRQWTLVADEDHEMPACQDAISDVDASKWSIGDVRELEVESIEFGRIAAQIAKQVIV
CHHHHEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
QKIREAERALIADAYESKVGELIYGEVKKQTKDGFIIDLGDNAEAYLAREEMIAKEILRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCH
KQRMNAILYSVNREGRGAQLLLSRARPEMLMALMKKEIPEISEEIIEIKAAARQPGVRAK
HHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAVKTNDHRIDPVGACIGMRGTRIQAVQQELNGERIDVVVWSDDPAQYIASALEPADVSG
EEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCE
IVLDEDTRSADIIFATNDQLARAIGSQGQNVRLASELTGYKLDMMLEEEYRARQQNEAQQ
EEEECCCCCCEEEEECCHHHHHHHCCCCCCEEEHHHHCCCEEEHEEHHHHHHHHHHHHHH
YLDMFVTRLDIEEDLAMALVEMGFTSLEEIAYVPPETFDEIELDQELVELLQSRAKEAAL
HHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TDALKQQENVQEPSVELLAMAGMTQELAYALAARGVVTVDDLADQATDDISDIEGLSEEK
HHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCEEHHHHHHHHCCCHHHHHCCCHHH
AGQLIMKARESWFN
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SREILTVVETVSNEKGVSREAIFEALEQALVAATKKKFYEGTNAEEAQLRVEIDRKTGD
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
YRTFRQWTLVADEDHEMPACQDAISDVDASKWSIGDVRELEVESIEFGRIAAQIAKQVIV
CHHHHEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
QKIREAERALIADAYESKVGELIYGEVKKQTKDGFIIDLGDNAEAYLAREEMIAKEILRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCH
KQRMNAILYSVNREGRGAQLLLSRARPEMLMALMKKEIPEISEEIIEIKAAARQPGVRAK
HHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAVKTNDHRIDPVGACIGMRGTRIQAVQQELNGERIDVVVWSDDPAQYIASALEPADVSG
EEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCE
IVLDEDTRSADIIFATNDQLARAIGSQGQNVRLASELTGYKLDMMLEEEYRARQQNEAQQ
EEEECCCCCCEEEEECCHHHHHHHCCCCCCEEEHHHHCCCEEEHEEHHHHHHHHHHHHHH
YLDMFVTRLDIEEDLAMALVEMGFTSLEEIAYVPPETFDEIELDQELVELLQSRAKEAAL
HHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TDALKQQENVQEPSVELLAMAGMTQELAYALAARGVVTVDDLADQATDDISDIEGLSEEK
HHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCEEHHHHHHHHCCCHHHHHCCCHHH
AGQLIMKARESWFN
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]