Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 50083625

GI number: 50083625

Start: 356605

End: 357402

Strand: Direct

Name: tpiA

Synonym: ACIAD0363

Alternate gene names: 50083625

Gene position: 356605-357402 (Clockwise)

Preceding gene: 50083620

Following gene: 50083626

Centisome position: 9.91

GC content: 43.61

Gene sequence:

>798_bases
ATGTCGGGCTCGACTATTATTCCTTGGGTTGTCGGCAATTGGAAAATGAATCCTAAACAATCTGATGCCATTCAACTTGT
ACAACAATTTAAAGATTTATTAAAGCAGCAACCTATTTCTGAGCAGTATTGTCATGTGGGTGTTGCACCTATAGCCATCG
CATTGACAACAATTCAAAGCGAACTGGCAACGGCAAACCGTCAAGTTGCGACGGTTGCGCAAGATGTTTCTCGTTTTGCT
GGTACAGGTGCTTATACAGGTGAAATAAGTGCCGAATTGCTGACAGATAGCCAAATTCGATATGTCCTGATTGGACATTC
TGAGCGTCGTGACTTACTTGGTGATCATGTTGAAATTTTAAAAGCAAAACTAAGCCATGCCTTAAATGCAGGAATGACAG
TCATTTATTGTGTAGGTGAAAGTCTTGAACAGCGTGAACAAGGTTTGGCTGAGCAGATTGTGTTGCAGCAAATCTGTGAT
ATTGCACCTGTAGTGAGTGCCGAGCAGTGGCAGCATCAAATCGTCATTGCTTATGAACCGATCTGGGCCATCGGTACAGG
CCGTACTGCATCACCAGAAGATGCGCAAGCCATTCATGCCAAAATTCGTGAAGGTTTATGTCAGATTACCCCAGCAGGCT
CTCAAATTGCATTACTTTATGGTGGTAGTGTCAAGCCAGAAAATGCGGTAGAGTTGGCGGCTTGTCCAGATATTAATGGG
GCATTGGTTGGCGGAGCATCACTCAATGCTGAGTCATTTTATAAAATTGCTCAAGCATTTGCCCAAACGCAACAATAG

Upstream 100 bases:

>100_bases
AATTCACTTCGGTGAATAAGAAGGGTGTGAAAAGAAAACGTTCTAATTTTGCCAAAAACCCGTGTAGAATGTGCTGCATT
TTCGATGAAAGGTTAGCAAT

Downstream 100 bases:

>100_bases
GAGTTCAGCATGCATACGTTTGTATTGGTTGTACATATCATTTTGGCTGTACTAATTATTGGTCTCGTGTTGATTCAACA
CGGTAAAGGTGCTGATGCCG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSGSTIIPWVVGNWKMNPKQSDAIQLVQQFKDLLKQQPISEQYCHVGVAPIAIALTTIQSELATANRQVATVAQDVSRFA
GTGAYTGEISAELLTDSQIRYVLIGHSERRDLLGDHVEILKAKLSHALNAGMTVIYCVGESLEQREQGLAEQIVLQQICD
IAPVVSAEQWQHQIVIAYEPIWAIGTGRTASPEDAQAIHAKIREGLCQITPAGSQIALLYGGSVKPENAVELAACPDING
ALVGGASLNAESFYKIAQAFAQTQQ

Sequences:

>Translated_265_residues
MSGSTIIPWVVGNWKMNPKQSDAIQLVQQFKDLLKQQPISEQYCHVGVAPIAIALTTIQSELATANRQVATVAQDVSRFA
GTGAYTGEISAELLTDSQIRYVLIGHSERRDLLGDHVEILKAKLSHALNAGMTVIYCVGESLEQREQGLAEQIVLQQICD
IAPVVSAEQWQHQIVIAYEPIWAIGTGRTASPEDAQAIHAKIREGLCQITPAGSQIALLYGGSVKPENAVELAACPDING
ALVGGASLNAESFYKIAQAFAQTQQ
>Mature_264_residues
SGSTIIPWVVGNWKMNPKQSDAIQLVQQFKDLLKQQPISEQYCHVGVAPIAIALTTIQSELATANRQVATVAQDVSRFAG
TGAYTGEISAELLTDSQIRYVLIGHSERRDLLGDHVEILKAKLSHALNAGMTVIYCVGESLEQREQGLAEQIVLQQICDI
APVVSAEQWQHQIVIAYEPIWAIGTGRTASPEDAQAIHAKIREGLCQITPAGSQIALLYGGSVKPENAVELAACPDINGA
LVGGASLNAESFYKIAQAFAQTQQ

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI1790353, Length=259, Percent_Identity=36.6795366795367, Blast_Score=160, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17536593, Length=253, Percent_Identity=40.7114624505929, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320255, Length=245, Percent_Identity=35.9183673469388, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28572008, Length=244, Percent_Identity=38.9344262295082, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI28572006, Length=244, Percent_Identity=38.9344262295082, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI28572004, Length=244, Percent_Identity=38.9344262295082, Blast_Score=161, Evalue=5e-40,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_ACIAD (Q6FF44)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045135.1
- HSSP:   P36204
- ProteinModelPortal:   Q6FF44
- SMR:   Q6FF44
- STRING:   Q6FF44
- GeneID:   2880725
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0363
- NMPDR:   fig|62977.3.peg.879
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   IEKNGTM
- ProtClustDB:   PRK00042
- BioCyc:   ASP62977:ACIAD0363-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28516; Mature: 28385

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 106-106 ACT_SITE 179-179 BINDING 13-13 BINDING 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSTIIPWVVGNWKMNPKQSDAIQLVQQFKDLLKQQPISEQYCHVGVAPIAIALTTIQS
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
ELATANRQVATVAQDVSRFAGTGAYTGEISAELLTDSQIRYVLIGHSERRDLLGDHVEIL
HHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
KAKLSHALNAGMTVIYCVGESLEQREQGLAEQIVLQQICDIAPVVSAEQWQHQIVIAYEP
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECC
IWAIGTGRTASPEDAQAIHAKIREGLCQITPAGSQIALLYGGSVKPENAVELAACPDING
EEEECCCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCC
ALVGGASLNAESFYKIAQAFAQTQQ
EEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGSTIIPWVVGNWKMNPKQSDAIQLVQQFKDLLKQQPISEQYCHVGVAPIAIALTTIQS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
ELATANRQVATVAQDVSRFAGTGAYTGEISAELLTDSQIRYVLIGHSERRDLLGDHVEIL
HHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
KAKLSHALNAGMTVIYCVGESLEQREQGLAEQIVLQQICDIAPVVSAEQWQHQIVIAYEP
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECC
IWAIGTGRTASPEDAQAIHAKIREGLCQITPAGSQIALLYGGSVKPENAVELAACPDING
EEEECCCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCC
ALVGGASLNAESFYKIAQAFAQTQQ
EEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA