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The map label for this gene is rlmB

Identifier: 50083619

GI number: 50083619

Start: 350219

End: 350965

Strand: Direct

Name: rlmB

Synonym: ACIAD0357

Alternate gene names: 50083619

Gene position: 350219-350965 (Clockwise)

Preceding gene: 50083618

Following gene: 50083620

Centisome position: 9.73

GC content: 46.59

Gene sequence:

>747_bases
ATGGCAAAACCAGAATATTACTATGGCGTTCATTCGGTGGAGTCATTGTTAGAATTAGAACCCGAACGTGTTTTAACACT
GTTTACTTTAAAAGGTCGTGATGACCAGCGCTTGCAAAAGATTCTGGAGCTGGCGGAACCTTTTGGTATCAGTGTACAAA
AGGCGAGTCGTGACAGTCTTGAAAAGCTGGCTGGCCTTCCTTTTCATCAGGGCGTTGTTGCTGCAGTGCGCCCTCATCCT
GTACTCAATGAAAAAGATTTAGATCAGCTTTTACAAAATAATGATCAAGCATTGTTGCTCGCGCTTGATCAGGTGACCGA
TCCACATAATCTGGGGGCTTGCATTCGAACAGCCGCTGCGATGGGCATTGCTGCGGTGATTGTGCCACGTGATCGCTCAG
CGAGTCTGACACCGACAGCACGTAAAGTTGCGGCAGGTGGGGCAGAAAAGGTCAAATTTATTCAAGTGACCAATCTGGCA
CGGACCTTGGCGCATATCAAAGCGCATTTTTTTGTGAAAGTGGTCGGCACCATGCTGGATGAAAAAGCACTTCCGATTCA
GAAATATGATTTTTCTGGCAACGTTGCGATTGTGATGGGGGCTGAAGATACAGGTTTACGTCCAATCACACAGTCTCAAT
GCGATCAAACCGTTTATATTCCAATGTCAGGCAATTTGCAAAGTTTGAATGTGAGTGTCGCAGCAGGAATGGCATTATAT
GAGGCATGTCGTCAGCGCCTCGGCTAA

Upstream 100 bases:

>100_bases
ACTCTTCGGCTGTTAAATCAGTTAAAATAGTTGTGTTAAAAATCCTGTGAAGCTGGCTTTACAGGATTTTATTTTTTATA
TGCGGTTTTAGAGGTTAAAC

Downstream 100 bases:

>100_bases
AACGCTATTTCGCTTCTCTTTTGTTGCTTAAGAAGGTCTTATGTCACCGCGTTTTCAAGGTTATTGTTTTGTCGCGATAA
CAATGTGTATTTGGGGGGGA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MAKPEYYYGVHSVESLLELEPERVLTLFTLKGRDDQRLQKILELAEPFGISVQKASRDSLEKLAGLPFHQGVVAAVRPHP
VLNEKDLDQLLQNNDQALLLALDQVTDPHNLGACIRTAAAMGIAAVIVPRDRSASLTPTARKVAAGGAEKVKFIQVTNLA
RTLAHIKAHFFVKVVGTMLDEKALPIQKYDFSGNVAIVMGAEDTGLRPITQSQCDQTVYIPMSGNLQSLNVSVAAGMALY
EACRQRLG

Sequences:

>Translated_248_residues
MAKPEYYYGVHSVESLLELEPERVLTLFTLKGRDDQRLQKILELAEPFGISVQKASRDSLEKLAGLPFHQGVVAAVRPHP
VLNEKDLDQLLQNNDQALLLALDQVTDPHNLGACIRTAAAMGIAAVIVPRDRSASLTPTARKVAAGGAEKVKFIQVTNLA
RTLAHIKAHFFVKVVGTMLDEKALPIQKYDFSGNVAIVMGAEDTGLRPITQSQCDQTVYIPMSGNLQSLNVSVAAGMALY
EACRQRLG
>Mature_247_residues
AKPEYYYGVHSVESLLELEPERVLTLFTLKGRDDQRLQKILELAEPFGISVQKASRDSLEKLAGLPFHQGVVAAVRPHPV
LNEKDLDQLLQNNDQALLLALDQVTDPHNLGACIRTAAAMGIAAVIVPRDRSASLTPTARKVAAGGAEKVKFIQVTNLAR
TLAHIKAHFFVKVVGTMLDEKALPIQKYDFSGNVAIVMGAEDTGLRPITQSQCDQTVYIPMSGNLQSLNVSVAAGMALYE
ACRQRLG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily

Homologues:

Organism=Homo sapiens, GI40068479, Length=226, Percent_Identity=31.4159292035398, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=47.9338842975207, Blast_Score=223, Evalue=8e-60,
Organism=Escherichia coli, GI1790083, Length=160, Percent_Identity=34.375, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788935, Length=239, Percent_Identity=27.6150627615063, Blast_Score=80, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324775, Length=290, Percent_Identity=24.8275862068966, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMB_ACIAD (Q6FF50)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045129.1
- ProteinModelPortal:   Q6FF50
- SMR:   Q6FF50
- STRING:   Q6FF50
- GeneID:   2881158
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0357
- NMPDR:   fig|62977.3.peg.936
- eggNOG:   COG0566
- HOGENOM:   HBG714057
- OMA:   LIRIPTS
- PhylomeDB:   Q6FF50
- ProtClustDB:   CLSK706925
- BioCyc:   ASP62977:ACIAD0357-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01887
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26985; Mature: 26853

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: BINDING 200-200 BINDING 220-220 BINDING 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPEYYYGVHSVESLLELEPERVLTLFTLKGRDDQRLQKILELAEPFGISVQKASRDSL
CCCCCCEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCHHHH
EKLAGLPFHQGVVAAVRPHPVLNEKDLDQLLQNNDQALLLALDQVTDPHNLGACIRTAAA
HHHHCCCHHCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
MGIAAVIVPRDRSASLTPTARKVAAGGAEKVKFIQVTNLARTLAHIKAHFFVKVVGTMLD
HCEEEEEEECCCCCCCCCHHHHHHCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
EKALPIQKYDFSGNVAIVMGAEDTGLRPITQSQCDQTVYIPMSGNLQSLNVSVAAGMALY
CCCCCCEEECCCCCEEEEECCCCCCCCCCCHHHCCCEEEEEECCCEEEEEHHHHHHHHHH
EACRQRLG
HHHHHHCC
>Mature Secondary Structure 
AKPEYYYGVHSVESLLELEPERVLTLFTLKGRDDQRLQKILELAEPFGISVQKASRDSL
CCCCCEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCHHHH
EKLAGLPFHQGVVAAVRPHPVLNEKDLDQLLQNNDQALLLALDQVTDPHNLGACIRTAAA
HHHHCCCHHCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
MGIAAVIVPRDRSASLTPTARKVAAGGAEKVKFIQVTNLARTLAHIKAHFFVKVVGTMLD
HCEEEEEEECCCCCCCCCHHHHHHCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
EKALPIQKYDFSGNVAIVMGAEDTGLRPITQSQCDQTVYIPMSGNLQSLNVSVAAGMALY
CCCCCCEEECCCCCEEEEECCCCCCCCCCCHHHCCCEEEEEECCCEEEEEHHHHHHHHHH
EACRQRLG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA