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The map label for this gene is thiC

Identifier: 50083553

GI number: 50083553

Start: 275899

End: 277794

Strand: Reverse

Name: thiC

Synonym: ACIAD0276

Alternate gene names: 50083553

Gene position: 277794-275899 (Counterclockwise)

Preceding gene: 50083556

Following gene: 50083552

Centisome position: 7.72

GC content: 45.99

Gene sequence:

>1896_bases
ATGAACCAATTAACGAATCTGTCCCCTGCTGACCTCTCAGCTCAACATGAACAAGATGCCAAGGATCTCACCCGTATTCT
GCCCGCATCCACTAAAGTTTATATTCAAGGTTCCCGACCAGATATTCAGGTGCCCATGCGCCAGATCGCACTGACAGACA
CACCAACCGGTCTAGGCGGCGAAAAAAACCCACCTGTGATGGTTTATGACACTTCGGGCGTCTATACCGACCCTGAGGTT
GCGATTGATTTAAACAAGGGTTTACCTCATGTTCGTCAGGCCTGGATTGAAGAGCGACAAGATACCGATGTACTCGACAC
TTTGAGCTCCAGCTTTGGACAAGAACGCCTGAAAGATATCCGTACTGCAGATATCCGATTTGCACATATCCAGAAACCAC
GTCGTGCCAAAACAGGACAAAACGTCACGCAAATGCACTATGCCAAAAAGGGCATGATCACCCCTGAAATGGAATATATC
GCGATTCGTGAAAACCAGCGTCAGCGTGACGAAGTAGACATGCGTCAACATGCAGGACAAAACTTCGGAGCAAAGAATCT
GACCGAAATTACCCCTGAGTTTGTTCGCCAAGAAGTGGCTGCTGGACGTGCCATTATTCCTGCCAATATTAATCATCCTG
AAATCGAACCCATGATTATTGGTCGCAACTTTCTGGTGAAGATCAATGCCAATATCGGTAACTCGGCATTGGGTTCGAGT
ATTGATGAAGAAGTGTCAAAAATGACCTGGGCGACCCGCTGGGGTGCAGATACCATCATGGATCTTTCCACAGGTAAAAA
TATTCACGAAACCCGTGAATGGATTATCCGCAACTCGCCTGTTCCGATTGGAACCGTTCCAATCTATCAGGCACTCGAAA
AAGTCGATGGTGTAGCAGAAGATCTAACTTGGGAAATTTTTAAAGACACCTTAATTGAACAGGCAGAACAGGGGGTCGAT
TATTTTACGATTCATGCGGGTGTATTATTACGCTACGTTCCTTTAACAGCCAATCGCCTGACCGGTATTGTGTCACGCGG
TGGTTCAATTATGGCGCAGTGGTGTCTGGCACATCATGAAGAAAACTTCCTCTACACGCATTTTGATGAAATCTGCGAGA
TTATGAAGGCCTATGACGTGTCATTTAGTCTGGGTGATGGACTCCGCCCTGGCTGTATTCAAGATGCCAACGATGAAGCA
CAGTTTGGCGAACTCCGTACACTCGGTGAGTTGACACATCGTGCATGGGAACATGATGTACAGGTCATGATTGAAGGGCC
AGGACACGTACCGATGCATATGATCAAGGAAAATATGGATTTACAGCTTGAAGTCTGTAAGGAAGCACCATTTTATACTT
TAGGGCCACTCACTACCGATATTGCACCTGGTTATGATCATATTACTTCAGCCATTGGTGCAGCCATGATTGGCTGGTAT
GGTACAGCCATGCTGTGTTATGTCACTCCGAAAGAGCATTTAGGCCTACCCAACAAGAAAGACGTCAAAGATGGGATTAT
CACTTACAAGATTGCCGCCCATGCTGCCGATCTTGCTAAAGGGCATCCAGGTGCGCAGGTACGTGACAATGCCTTATCTA
AAGCCCGTTTCGAATTCCGTTGGGATGACCAGTTCAATTTGAGTCTAGATCCAGATACTGCCCGTAGTATGCACGATGAA
ACGCTTCCAAAAGAGGCACATAAATCTGCGCATTTCTGCTCGATGTGCGGTCCTAAATTTTGCTCCATGAAAATCACTCA
AAATGTGCGAAACTATGCACAAAATCAAGAGACTGCAAAGGATTTCAATCCATCTACCGAAGAGGTTGAACAAGGTCTCA
AGAGCATGAAACAGGCTTATCACGACAACGGTCAAAAACTGTATCAAAAAGTCTAA

Upstream 100 bases:

>100_bases
CAAGTCATCAAAAAACATATGCCCTCTATCTGCCTATCAGATACTTTTGCCATCGTTTTTGGTTGCTTGATTCGTTAGTG
TTTTTCATAAGGATCATGCC

Downstream 100 bases:

>100_bases
AACGCTAAAAAAAGCGTTTGTCTTTAATTTGAACTCAGTTAGGATGAAATACAGACATGTATTTCATCCTTTTTAAATTT
TATGACCATATTAGAACAGG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MNQLTNLSPADLSAQHEQDAKDLTRILPASTKVYIQGSRPDIQVPMRQIALTDTPTGLGGEKNPPVMVYDTSGVYTDPEV
AIDLNKGLPHVRQAWIEERQDTDVLDTLSSSFGQERLKDIRTADIRFAHIQKPRRAKTGQNVTQMHYAKKGMITPEMEYI
AIRENQRQRDEVDMRQHAGQNFGAKNLTEITPEFVRQEVAAGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSS
IDEEVSKMTWATRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGVAEDLTWEIFKDTLIEQAEQGVD
YFTIHAGVLLRYVPLTANRLTGIVSRGGSIMAQWCLAHHEENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIQDANDEA
QFGELRTLGELTHRAWEHDVQVMIEGPGHVPMHMIKENMDLQLEVCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWY
GTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFNLSLDPDTARSMHDE
TLPKEAHKSAHFCSMCGPKFCSMKITQNVRNYAQNQETAKDFNPSTEEVEQGLKSMKQAYHDNGQKLYQKV

Sequences:

>Translated_631_residues
MNQLTNLSPADLSAQHEQDAKDLTRILPASTKVYIQGSRPDIQVPMRQIALTDTPTGLGGEKNPPVMVYDTSGVYTDPEV
AIDLNKGLPHVRQAWIEERQDTDVLDTLSSSFGQERLKDIRTADIRFAHIQKPRRAKTGQNVTQMHYAKKGMITPEMEYI
AIRENQRQRDEVDMRQHAGQNFGAKNLTEITPEFVRQEVAAGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSS
IDEEVSKMTWATRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGVAEDLTWEIFKDTLIEQAEQGVD
YFTIHAGVLLRYVPLTANRLTGIVSRGGSIMAQWCLAHHEENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIQDANDEA
QFGELRTLGELTHRAWEHDVQVMIEGPGHVPMHMIKENMDLQLEVCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWY
GTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFNLSLDPDTARSMHDE
TLPKEAHKSAHFCSMCGPKFCSMKITQNVRNYAQNQETAKDFNPSTEEVEQGLKSMKQAYHDNGQKLYQKV
>Mature_631_residues
MNQLTNLSPADLSAQHEQDAKDLTRILPASTKVYIQGSRPDIQVPMRQIALTDTPTGLGGEKNPPVMVYDTSGVYTDPEV
AIDLNKGLPHVRQAWIEERQDTDVLDTLSSSFGQERLKDIRTADIRFAHIQKPRRAKTGQNVTQMHYAKKGMITPEMEYI
AIRENQRQRDEVDMRQHAGQNFGAKNLTEITPEFVRQEVAAGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSS
IDEEVSKMTWATRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGVAEDLTWEIFKDTLIEQAEQGVD
YFTIHAGVLLRYVPLTANRLTGIVSRGGSIMAQWCLAHHEENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIQDANDEA
QFGELRTLGELTHRAWEHDVQVMIEGPGHVPMHMIKENMDLQLEVCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWY
GTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFNLSLDPDTARSMHDE
TLPKEAHKSAHFCSMCGPKFCSMKITQNVRNYAQNQETAKDFNPSTEEVEQGLKSMKQAYHDNGQKLYQKV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=627, Percent_Identity=68.5805422647528, Blast_Score=897, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_ACIAD (Q6FFB6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045063.1
- STRING:   Q6FFB6
- GeneID:   2880744
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0276
- NMPDR:   fig|62977.3.peg.617
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q6FFB6
- ProtClustDB:   PRK09284
- BioCyc:   ASP62977:ACIAD0276-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 70794; Mature: 70794

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: BINDING 231-231 BINDING 260-260 BINDING 289-289 BINDING 325-325 BINDING 425-425 BINDING 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLTNLSPADLSAQHEQDAKDLTRILPASTKVYIQGSRPDIQVPMRQIALTDTPTGLGG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHEEECCCCCCCCC
EKNPPVMVYDTSGVYTDPEVAIDLNKGLPHVRQAWIEERQDTDVLDTLSSSFGQERLKDI
CCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RTADIRFAHIQKPRRAKTGQNVTQMHYAKKGMITPEMEYIAIRENQRQRDEVDMRQHAGQ
HHHCEEEHHCCCCHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCC
NFGAKNLTEITPEFVRQEVAAGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSS
CCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHCCH
IDEEVSKMTWATRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGVAE
HHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHH
DLTWEIFKDTLIEQAEQGVDYFTIHAGVLLRYVPLTANRLTGIVSRGGSIMAQWCLAHHE
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCC
ENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIQDANDEAQFGELRTLGELTHRAWEHDV
CCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
QVMIEGPGHVPMHMIKENMDLQLEVCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWY
EEEEECCCCCCHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQVRDNALSKARFEFR
HHEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEEEE
WDDQFNLSLDPDTARSMHDETLPKEAHKSAHFCSMCGPKFCSMKITQNVRNYAQNQETAK
ECCCEEEEECCHHHHHHHHHCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHH
DFNPSTEEVEQGLKSMKQAYHDNGQKLYQKV
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure
MNQLTNLSPADLSAQHEQDAKDLTRILPASTKVYIQGSRPDIQVPMRQIALTDTPTGLGG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHEEECCCCCCCCC
EKNPPVMVYDTSGVYTDPEVAIDLNKGLPHVRQAWIEERQDTDVLDTLSSSFGQERLKDI
CCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RTADIRFAHIQKPRRAKTGQNVTQMHYAKKGMITPEMEYIAIRENQRQRDEVDMRQHAGQ
HHHCEEEHHCCCCHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCC
NFGAKNLTEITPEFVRQEVAAGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSS
CCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHCCH
IDEEVSKMTWATRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGVAE
HHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHH
DLTWEIFKDTLIEQAEQGVDYFTIHAGVLLRYVPLTANRLTGIVSRGGSIMAQWCLAHHE
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCC
ENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIQDANDEAQFGELRTLGELTHRAWEHDV
CCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
QVMIEGPGHVPMHMIKENMDLQLEVCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWY
EEEEECCCCCCHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQVRDNALSKARFEFR
HHEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEEEE
WDDQFNLSLDPDTARSMHDETLPKEAHKSAHFCSMCGPKFCSMKITQNVRNYAQNQETAK
ECCCEEEEECCHHHHHHHHHCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHH
DFNPSTEEVEQGLKSMKQAYHDNGQKLYQKV
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA