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The map label for this gene is btuE [H]

Identifier: 50083475

GI number: 50083475

Start: 188463

End: 189008

Strand: Reverse

Name: btuE [H]

Synonym: ACIAD0193

Alternate gene names: 50083475

Gene position: 189008-188463 (Counterclockwise)

Preceding gene: 50083476

Following gene: 50083474

Centisome position: 5.25

GC content: 40.66

Gene sequence:

>546_bases
ATGAGCCAATCTATTTATCATATTCCTGTACAGACCATCTCTGGTCAAGACACGTCATTAGACACCTATCGAGGCAAGGT
GCTTCTTATTGTCAATGTTGCTTCAAAATGTGGTTTGACACCCCAGTATGAAGGTCTGGAAAAACTTTATCAGGATAAAC
AACAACAAGGTTTGGAAATTTTAGGATTCCCAGCCAATAACTTTTTAGAACAAGAGCCAGGCTCAAATGATGAAATCCAG
CAATTTTGCTCTTTGACTTATGATGTGCATTTTCCGCTATTTGGCAAAATTTCGGTGGCAGGTGAAGATAAACACCCGCT
TTATCAAACACTCATTGAAGCACAGCCAGAACGTATTGGCGAAGGCCCTTGGTGGAAAGATCTGGTCGATTATGGTTTGA
CACCTAACCCAAAACCAGAAGTTTTATGGAACTTCGAAAAATTTCTAATCAATAAAAATGGGGAAGTCGTTGCGCGTTTT
GCTCCAGACATTACTGCTGATGACCCACGTTTGGTCGATGCAATTAATGCGGAACTTGCCAAATAA

Upstream 100 bases:

>100_bases
CATAGCCAGGATTTAGGCCTGTGTCCAATCGAGACAAAAGCCTTCACTTGATGAAAAATAATTTTTTTAATTACACTCCA
CTTAACACGAAGGATCGCTC

Downstream 100 bases:

>100_bases
GATATTTTAAACATGTGCATGATCTATCATGCACATGTTTTATGTCTGGTCTACAGCAATATTTTTACAACATGAACTTC
ATATTGCTTCAATCTCATCA

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSQSIYHIPVQTISGQDTSLDTYRGKVLLIVNVASKCGLTPQYEGLEKLYQDKQQQGLEILGFPANNFLEQEPGSNDEIQ
QFCSLTYDVHFPLFGKISVAGEDKHPLYQTLIEAQPERIGEGPWWKDLVDYGLTPNPKPEVLWNFEKFLINKNGEVVARF
APDITADDPRLVDAINAELAK

Sequences:

>Translated_181_residues
MSQSIYHIPVQTISGQDTSLDTYRGKVLLIVNVASKCGLTPQYEGLEKLYQDKQQQGLEILGFPANNFLEQEPGSNDEIQ
QFCSLTYDVHFPLFGKISVAGEDKHPLYQTLIEAQPERIGEGPWWKDLVDYGLTPNPKPEVLWNFEKFLINKNGEVVARF
APDITADDPRLVDAINAELAK
>Mature_180_residues
SQSIYHIPVQTISGQDTSLDTYRGKVLLIVNVASKCGLTPQYEGLEKLYQDKQQQGLEILGFPANNFLEQEPGSNDEIQQ
FCSLTYDVHFPLFGKISVAGEDKHPLYQTLIEAQPERIGEGPWWKDLVDYGLTPNPKPEVLWNFEKFLINKNGEVVARFA
PDITADDPRLVDAINAELAK

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=169, Percent_Identity=36.6863905325444, Blast_Score=112, Evalue=1e-25,
Organism=Homo sapiens, GI75709200, Length=162, Percent_Identity=40.1234567901235, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI90903240, Length=162, Percent_Identity=40.1234567901235, Blast_Score=111, Evalue=3e-25,
Organism=Homo sapiens, GI192455698, Length=161, Percent_Identity=34.1614906832298, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI90903238, Length=148, Percent_Identity=37.8378378378378, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI41406084, Length=183, Percent_Identity=36.0655737704918, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI6006001, Length=184, Percent_Identity=34.7826086956522, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI4557629, Length=161, Percent_Identity=34.1614906832298, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI32967607, Length=170, Percent_Identity=31.7647058823529, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI33186887, Length=167, Percent_Identity=31.1377245508982, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=49.1803278688525, Blast_Score=186, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17550320, Length=172, Percent_Identity=37.2093023255814, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI193209493, Length=170, Percent_Identity=35.2941176470588, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17506887, Length=160, Percent_Identity=37.5, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17535473, Length=168, Percent_Identity=38.0952380952381, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71993584, Length=166, Percent_Identity=30.7228915662651, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193210707, Length=164, Percent_Identity=35.3658536585366, Blast_Score=93, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71999642, Length=78, Percent_Identity=43.5897435897436, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319721, Length=162, Percent_Identity=41.9753086419753, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322228, Length=162, Percent_Identity=43.2098765432099, Blast_Score=126, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322826, Length=148, Percent_Identity=37.1621621621622, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24656767, Length=180, Percent_Identity=37.2222222222222, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24656777, Length=180, Percent_Identity=37.2222222222222, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24656772, Length=180, Percent_Identity=37.2222222222222, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI85726443, Length=163, Percent_Identity=34.9693251533742, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20380; Mature: 20249

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSIYHIPVQTISGQDTSLDTYRGKVLLIVNVASKCGLTPQYEGLEKLYQDKQQQGLEI
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHCCCEE
LGFPANNFLEQEPGSNDEIQQFCSLTYDVHFPLFGKISVAGEDKHPLYQTLIEAQPERIG
EECCCCCHHCCCCCCCHHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
EGPWWKDLVDYGLTPNPKPEVLWNFEKFLINKNGEVVARFAPDITADDPRLVDAINAELA
CCCCHHHHHHCCCCCCCCCCEEECHHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHC
K
C
>Mature Secondary Structure 
SQSIYHIPVQTISGQDTSLDTYRGKVLLIVNVASKCGLTPQYEGLEKLYQDKQQQGLEI
CCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHCCCEE
LGFPANNFLEQEPGSNDEIQQFCSLTYDVHFPLFGKISVAGEDKHPLYQTLIEAQPERIG
EECCCCCHHCCCCCCCHHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
EGPWWKDLVDYGLTPNPKPEVLWNFEKFLINKNGEVVARFAPDITADDPRLVDAINAELA
CCCCHHHHHHCCCCCCCCCCEEECHHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]