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The map label for this gene is garD [H]

Identifier: 50083415

GI number: 50083415

Start: 128937

End: 130484

Strand: Reverse

Name: garD [H]

Synonym: ACIAD0126

Alternate gene names: 50083415

Gene position: 130484-128937 (Counterclockwise)

Preceding gene: 50083416

Following gene: 50083414

Centisome position: 3.63

GC content: 41.21

Gene sequence:

>1548_bases
ATGTTAAAGCCAATTCTAATTCAAATTAATCCACTTGATAATGTCGCAATAGTGGTCAACGACCATGGTTTAGAAAAACA
AACCGTTATTGAAGAATATCAACTAACTTTGGTCGATGATGTTCCTCAAGGACATAAAGTATTATTAAAAGCATTAAAAA
AAGATGAGGCAATTATTCGCTATGGTGAAATCATCGGATATGCAAATGCAGATCTGGAAGCAGGTCGTTGGGTTAATGAA
TCTGTAACCCGTATGCCTGAGGCACCAGAGCTCGACAAACTTGAACTGGCAACTCGTCCTGCACAGCCGTTAGAATCTCT
TACAGGATTTACTTTTAAAGGCTATAAAAATAAAGATGGCAGCGTTGGAACCAAAAATATTTTAGGGATTACCACCAGCG
TGAACTGTGTAGAAGGTATTGTCGATTATGTGGTTAAAATTATAGAGCGAGAACTATTATCTAAATATCCAAATGTTGAT
GGTGTAGTCGGATTAAATCATTTATATGGTTGTGGCGTCGCCATTGATGCACCTGCGGCTATTGTCCCAATTCGTACCAT
TCATAATCTGGCGCTAAATCCAAATTTTGGCGGTGAAATTATGGTAGTCAGTTTAGGCTGCGAAAAAATGCAACCCGAAC
GTCTTTTAAATATGACCGCAGAAAACAAACGTATTGCCTTAGCAGATGAAGATATTATTCAGTTGCAAGATGAACGACAT
CACGGCTTTGAAAGTATGGTCGCACATATTTTAACGGTGGCAGAACAGCATCTTGAAAAATTGAACCAGCGCCAGCGTGA
AACTGTAGATGCATCACATTTAGTGGTTGGTATGCAATGCGGCGGTAGTGATGCTTTTTCAGGTGTAACGTCAAATCCAG
CAGTGGGTTTTGCAACAGATTTAATTGTTCGCTGTGGAGGAACGGTCATGTTTTCAGAAGTTACTGAAGTCCGCGACGGT
ATTCATTTACTTACCCCACGTGCAGCAAATGAAGATGTCGCTAAAGATCTCATTCGAGAAATGCAATGGTACGATGACTA
CTTAACTGCAGGTAAAGTAGACCGTAGTGCCAACACCACTCCTGGCAATAAAAAGGGTGGACTGAATAATATTGTAGAAA
AGGCCATGGGCTCAATTGCCAAGTCGGGTACATCTTCAATTGTTGAGGTGTTATCACCAGGCCAAAGACCCAGTAAAAAG
GGCTTAATCTTTGCAGCAACGCCATCAAGTGATTTTATTTGCGGTACACAACAAATGGCCTCAGGAATTACGGTTCAGGT
TTTTACAACTGGACGCGGTACACCCTACGGATTAGCTGCGGTTCCTGTCATTAAAATGGCCAGTCGTAACAACATTGCAA
ATCGCTGGTACGACTTAATTGATATTAGTGCTGGAGATATTGCAGTAGGTAAAAAAACCATTGAAGACGTTGGCTGGGAG
CTCTTTGAAATGATTTTACGTGTCGCGAGTGGTGAACAACAAACCTGGTCTGATCGCTGGGGCATTCATAATTCTCTTGC
AGTATTTAATCCTGCTCCTGTTACCTGA

Upstream 100 bases:

>100_bases
TTGAACGCTTTGAACTCAAAAAAGTCATATAAGTGATTTTGACCAGAAAGCTTAGGACGCAAGCTTTCTGGTTCTCTTAG
TTTTTTCAAGGAAGATCATT

Downstream 100 bases:

>100_bases
TGGAATAGATATTGTTAAACTACCTATATATTTTTAGTCTGCCTTATTTTTATGCAAGATATTTACTCAGCTCCGCCCTC
TCAAGTTGGTAAAGCCTTGT

Product: D-galactarate dehydrogenase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MLKPILIQINPLDNVAIVVNDHGLEKQTVIEEYQLTLVDDVPQGHKVLLKALKKDEAIIRYGEIIGYANADLEAGRWVNE
SVTRMPEAPELDKLELATRPAQPLESLTGFTFKGYKNKDGSVGTKNILGITTSVNCVEGIVDYVVKIIERELLSKYPNVD
GVVGLNHLYGCGVAIDAPAAIVPIRTIHNLALNPNFGGEIMVVSLGCEKMQPERLLNMTAENKRIALADEDIIQLQDERH
HGFESMVAHILTVAEQHLEKLNQRQRETVDASHLVVGMQCGGSDAFSGVTSNPAVGFATDLIVRCGGTVMFSEVTEVRDG
IHLLTPRAANEDVAKDLIREMQWYDDYLTAGKVDRSANTTPGNKKGGLNNIVEKAMGSIAKSGTSSIVEVLSPGQRPSKK
GLIFAATPSSDFICGTQQMASGITVQVFTTGRGTPYGLAAVPVIKMASRNNIANRWYDLIDISAGDIAVGKKTIEDVGWE
LFEMILRVASGEQQTWSDRWGIHNSLAVFNPAPVT

Sequences:

>Translated_515_residues
MLKPILIQINPLDNVAIVVNDHGLEKQTVIEEYQLTLVDDVPQGHKVLLKALKKDEAIIRYGEIIGYANADLEAGRWVNE
SVTRMPEAPELDKLELATRPAQPLESLTGFTFKGYKNKDGSVGTKNILGITTSVNCVEGIVDYVVKIIERELLSKYPNVD
GVVGLNHLYGCGVAIDAPAAIVPIRTIHNLALNPNFGGEIMVVSLGCEKMQPERLLNMTAENKRIALADEDIIQLQDERH
HGFESMVAHILTVAEQHLEKLNQRQRETVDASHLVVGMQCGGSDAFSGVTSNPAVGFATDLIVRCGGTVMFSEVTEVRDG
IHLLTPRAANEDVAKDLIREMQWYDDYLTAGKVDRSANTTPGNKKGGLNNIVEKAMGSIAKSGTSSIVEVLSPGQRPSKK
GLIFAATPSSDFICGTQQMASGITVQVFTTGRGTPYGLAAVPVIKMASRNNIANRWYDLIDISAGDIAVGKKTIEDVGWE
LFEMILRVASGEQQTWSDRWGIHNSLAVFNPAPVT
>Mature_515_residues
MLKPILIQINPLDNVAIVVNDHGLEKQTVIEEYQLTLVDDVPQGHKVLLKALKKDEAIIRYGEIIGYANADLEAGRWVNE
SVTRMPEAPELDKLELATRPAQPLESLTGFTFKGYKNKDGSVGTKNILGITTSVNCVEGIVDYVVKIIERELLSKYPNVD
GVVGLNHLYGCGVAIDAPAAIVPIRTIHNLALNPNFGGEIMVVSLGCEKMQPERLLNMTAENKRIALADEDIIQLQDERH
HGFESMVAHILTVAEQHLEKLNQRQRETVDASHLVVGMQCGGSDAFSGVTSNPAVGFATDLIVRCGGTVMFSEVTEVRDG
IHLLTPRAANEDVAKDLIREMQWYDDYLTAGKVDRSANTTPGNKKGGLNNIVEKAMGSIAKSGTSSIVEVLSPGQRPSKK
GLIFAATPSSDFICGTQQMASGITVQVFTTGRGTPYGLAAVPVIKMASRNNIANRWYDLIDISAGDIAVGKKTIEDVGWE
LFEMILRVASGEQQTWSDRWGIHNSLAVFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=509, Percent_Identity=69.7445972495088, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=518, Percent_Identity=31.4671814671815, Blast_Score=218, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 56009; Mature: 56009

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPILIQINPLDNVAIVVNDHGLEKQTVIEEYQLTLVDDVPQGHKVLLKALKKDEAIIR
CCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHEE
YGEIIGYANADLEAGRWVNESVTRMPEAPELDKLELATRPAQPLESLTGFTFKGYKNKDG
HHHHEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCHHHHHHCCCEEECCCCCCC
SVGTKNILGITTSVNCVEGIVDYVVKIIERELLSKYPNVDGVVGLNHLYGCGVAIDAPAA
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHCCCEEECCCCC
IVPIRTIHNLALNPNFGGEIMVVSLGCEKMQPERLLNMTAENKRIALADEDIIQLQDERH
EEEHHHHHHEEECCCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEECCCHHHHHCCHHC
HGFESMVAHILTVAEQHLEKLNQRQRETVDASHLVVGMQCGGSDAFSGVTSNPAVGFATD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCCCCCCCCCHHHHH
LIVRCGGTVMFSEVTEVRDGIHLLTPRAANEDVAKDLIREMQWYDDYLTAGKVDRSANTT
HHHHCCCEEEHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGNKKGGLNNIVEKAMGSIAKSGTSSIVEVLSPGQRPSKKGLIFAATPSSDFICGTQQMA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCEECHHHHH
SGITVQVFTTGRGTPYGLAAVPVIKMASRNNIANRWYDLIDISAGDIAVGKKTIEDVGWE
CCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHH
LFEMILRVASGEQQTWSDRWGIHNSLAVFNPAPVT
HHHHHHHHHCCCCCCCHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure
MLKPILIQINPLDNVAIVVNDHGLEKQTVIEEYQLTLVDDVPQGHKVLLKALKKDEAIIR
CCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHEE
YGEIIGYANADLEAGRWVNESVTRMPEAPELDKLELATRPAQPLESLTGFTFKGYKNKDG
HHHHEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCHHHHHHCCCEEECCCCCCC
SVGTKNILGITTSVNCVEGIVDYVVKIIERELLSKYPNVDGVVGLNHLYGCGVAIDAPAA
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHCCCEEECCCCC
IVPIRTIHNLALNPNFGGEIMVVSLGCEKMQPERLLNMTAENKRIALADEDIIQLQDERH
EEEHHHHHHEEECCCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEECCCHHHHHCCHHC
HGFESMVAHILTVAEQHLEKLNQRQRETVDASHLVVGMQCGGSDAFSGVTSNPAVGFATD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCCCCCCCCCHHHHH
LIVRCGGTVMFSEVTEVRDGIHLLTPRAANEDVAKDLIREMQWYDDYLTAGKVDRSANTT
HHHHCCCEEEHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGNKKGGLNNIVEKAMGSIAKSGTSSIVEVLSPGQRPSKKGLIFAATPSSDFICGTQQMA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCEECHHHHH
SGITVQVFTTGRGTPYGLAAVPVIKMASRNNIANRWYDLIDISAGDIAVGKKTIEDVGWE
CCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHH
LFEMILRVASGEQQTWSDRWGIHNSLAVFNPAPVT
HHHHHHHHHCCCCCCCHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]