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The map label for this gene is rmlB [H]

Identifier: 50083368

GI number: 50083368

Start: 72769

End: 73839

Strand: Direct

Name: rmlB [H]

Synonym: ACIAD0076

Alternate gene names: 50083368

Gene position: 72769-73839 (Clockwise)

Preceding gene: 50083367

Following gene: 50083369

Centisome position: 2.02

GC content: 40.15

Gene sequence:

>1071_bases
ATGATGAAAATATTAATAACAGGTGGTGCTGGTTTTATTGGTTCGGCAGTGGTACGTCATATTATTCAGAATACTGATAA
TACTGTACTTAATGTCGATAAACTAACTTATGCTGGTAATTTGGAGTCATTGGCGAAAGTCGCAGACAATTCACGCTATC
AGTTTTCTCAAACAGATATCTGTGATCGTGCGGCATTAGATCAGTTGTTTGAAACGTTTCAACCAGATGCTGTTATGCAC
CTGGCAGCAGAGTCACATGTTGACCGTTCGATTACAGGACCAGCAGCATTTATTGAAACCAATATTCTTGGAACTTATCA
GTTATTAGAAGCTGCAAGACATTATTGGAATGGTCTGGCACAAGATAAAAAAGCAGAGTTCCGCTTTCATCATATTTCAA
CAGATGAAGTGTATGGTGATTTGGAAGGTACAGAAGATTTATTTGTGGAAACCACACCATATGCACCGAGCTCTCCGTAT
TCTGCAAGTAAAGCCAGCTCTGACCATTTAGTACGTGCTTGGCATCGTACCTATGGCTTACCTGTGGTATTAACGAATTG
TTCTAATAACTATGGACCATACCACTTTCCAGAAAAGCTTATTCCGCTTGTTATTCTAAATGCACTTGCTGGCAAGCCTT
TACCTGTTTATGGGAATGGTGCCCAGATTCGTGATTGGTTGTATGTTGAAGATCATGCACGTGCCTTATATAAAGTTGTA
ACTGAAGCTAAGGTTGGAGAAACTTATAATATTGGTGGGCACAATGAACAGCAAAATATTGATGTCGTGAAAGCGATTTG
CAATTTACTTGAAGAGCTAGCACCCAATAAGCCAGAAGGTATTGCAAACTATCAGGATCTGATCACTTATGTAAAAGATC
GCCCTGGACATGACTTACGTTATGCCATTGATGCCAGTAAAATTAAGAAAGATCTGGGTTGGGTGCCTCAAGAGACTTTT
GAATCTGGATTGCGTAAAACAGTTGAATGGTATCTGGCAAATCAGGAATGGGTAAAGCATGTGCAAAGCGGCGAGTACCA
AAACTGGGTACAAAACCAATACCAAGCCTAG

Upstream 100 bases:

>100_bases
CAGAGTTGATTTTGGTTTTTTTATTTGCTTTATATTTGTTTTTCTATTTTAAGAGAAATAAATACTTGTAATTTTATTTT
AATTTTTTATCGGAGTAATA

Downstream 100 bases:

>100_bases
GTGAAAGAGATGGGTCACATGAAAATACTGATATTGGGCAAAAATGGACAAGTCGGCTGGGAATTACAACGATCGCTTCA
GCCCTTAGGTGAAGTTATTG

Product: dTDP-D-glucose-4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MMKILITGGAGFIGSAVVRHIIQNTDNTVLNVDKLTYAGNLESLAKVADNSRYQFSQTDICDRAALDQLFETFQPDAVMH
LAAESHVDRSITGPAAFIETNILGTYQLLEAARHYWNGLAQDKKAEFRFHHISTDEVYGDLEGTEDLFVETTPYAPSSPY
SASKASSDHLVRAWHRTYGLPVVLTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQIRDWLYVEDHARALYKVV
TEAKVGETYNIGGHNEQQNIDVVKAICNLLEELAPNKPEGIANYQDLITYVKDRPGHDLRYAIDASKIKKDLGWVPQETF
ESGLRKTVEWYLANQEWVKHVQSGEYQNWVQNQYQA

Sequences:

>Translated_356_residues
MMKILITGGAGFIGSAVVRHIIQNTDNTVLNVDKLTYAGNLESLAKVADNSRYQFSQTDICDRAALDQLFETFQPDAVMH
LAAESHVDRSITGPAAFIETNILGTYQLLEAARHYWNGLAQDKKAEFRFHHISTDEVYGDLEGTEDLFVETTPYAPSSPY
SASKASSDHLVRAWHRTYGLPVVLTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQIRDWLYVEDHARALYKVV
TEAKVGETYNIGGHNEQQNIDVVKAICNLLEELAPNKPEGIANYQDLITYVKDRPGHDLRYAIDASKIKKDLGWVPQETF
ESGLRKTVEWYLANQEWVKHVQSGEYQNWVQNQYQA
>Mature_356_residues
MMKILITGGAGFIGSAVVRHIIQNTDNTVLNVDKLTYAGNLESLAKVADNSRYQFSQTDICDRAALDQLFETFQPDAVMH
LAAESHVDRSITGPAAFIETNILGTYQLLEAARHYWNGLAQDKKAEFRFHHISTDEVYGDLEGTEDLFVETTPYAPSSPY
SASKASSDHLVRAWHRTYGLPVVLTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQIRDWLYVEDHARALYKVV
TEAKVGETYNIGGHNEQQNIDVVKAICNLLEELAPNKPEGIANYQDLITYVKDRPGHDLRYAIDASKIKKDLGWVPQETF
ESGLRKTVEWYLANQEWVKHVQSGEYQNWVQNQYQA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=37.2372372372372, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=27.0588235294118, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=77.8097982708934, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1788353, Length=363, Percent_Identity=72.7272727272727, Blast_Score=549, Evalue=1e-158,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=25.7790368271955, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788366, Length=244, Percent_Identity=28.6885245901639, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788365, Length=350, Percent_Identity=22.5714285714286, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=36.6863905325444, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=36.3636363636364, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=26.0355029585799, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=371, Percent_Identity=27.2237196765499, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=373, Percent_Identity=27.0777479892761, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI133901786, Length=298, Percent_Identity=25.1677852348993, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=298, Percent_Identity=25.1677852348993, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=298, Percent_Identity=25.1677852348993, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=298, Percent_Identity=25.1677852348993, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=298, Percent_Identity=25.1677852348993, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=286, Percent_Identity=25.1748251748252, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=26.3013698630137, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356223, Length=334, Percent_Identity=26.6467065868263, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=366, Percent_Identity=28.6885245901639, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24158427, Length=337, Percent_Identity=24.0356083086053, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40048; Mature: 40048

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKILITGGAGFIGSAVVRHIIQNTDNTVLNVDKLTYAGNLESLAKVADNSRYQFSQTDI
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCEEECCHHH
CDRAALDQLFETFQPDAVMHLAAESHVDRSITGPAAFIETNILGTYQLLEAARHYWNGLA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCC
QDKKAEFRFHHISTDEVYGDLEGTEDLFVETTPYAPSSPYSASKASSDHLVRAWHRTYGL
CCCCCCEEEEECCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PVVLTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQIRDWLYVEDHARALYKVV
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEHHHHHHHHHHH
TEAKVGETYNIGGHNEQQNIDVVKAICNLLEELAPNKPEGIANYQDLITYVKDRPGHDLR
HHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEE
YAIDASKIKKDLGWVPQETFESGLRKTVEWYLANQEWVKHVQSGEYQNWVQNQYQA
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MMKILITGGAGFIGSAVVRHIIQNTDNTVLNVDKLTYAGNLESLAKVADNSRYQFSQTDI
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCEEECCHHH
CDRAALDQLFETFQPDAVMHLAAESHVDRSITGPAAFIETNILGTYQLLEAARHYWNGLA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCC
QDKKAEFRFHHISTDEVYGDLEGTEDLFVETTPYAPSSPYSASKASSDHLVRAWHRTYGL
CCCCCCEEEEECCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PVVLTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQIRDWLYVEDHARALYKVV
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEHHHHHHHHHHH
TEAKVGETYNIGGHNEQQNIDVVKAICNLLEELAPNKPEGIANYQDLITYVKDRPGHDLR
HHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEE
YAIDASKIKKDLGWVPQETFESGLRKTVEWYLANQEWVKHVQSGEYQNWVQNQYQA
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]