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The map label for this gene is rph [H]

Identifier: 50083344

GI number: 50083344

Start: 54008

End: 54763

Strand: Direct

Name: rph [H]

Synonym: ACIAD0050

Alternate gene names: 50083344

Gene position: 54008-54763 (Clockwise)

Preceding gene: 50083343

Following gene: 50083345

Centisome position: 1.5

GC content: 44.71

Gene sequence:

>756_bases
ATGCTGCAATCTTATTTAGCGGCAATGAAAGGATGGTTTATGCGTCTTGACCAACGAGCACTTGATCAATTACGTGATGT
AAAAATTACGAGAAATTACACCCGTTATGCAGAAGGTTCGGTTTTGGTCGAATTTGGTCATACCAAGGTGTTATGTACCG
CAAGCATTGACAATTCTGTACCACGCTTTTTAAAAGGTCAGGGACAAGGCTGGGTGACTGCTGAATATGGCATGTTACCG
CGTTCAACCCATACGCGTAGCGACCGTGAAGCCGCACGTGGCAAGCAAACTGGTCGTACACAAGAGATTCAACGTTTGAT
TGGCCGTAGCTTGCGTGCCATGGTCGATTTGAAAAAACTCGGTGAAAATACGATTACCATTGACTGTGATGTGATTCAGG
CAGATGGTGGTACACGCACCGCAGCAATTACAGGCGCGGCAGTTGCGCTGATTGATGCCATGAATGTGCTATTAGAAAAG
AAAAAAATTAAGCAGGATCCACTTAAAGGTCTGGTGGCTGCAATTTCAGTCGGAATCTATCAGGATGAAGTGCTACTTGA
TCTTTGCTATGAAGAAGATTCAAACTGCCAGACAGATTTAAATGTCGTGATGACTCAGGCGGGTGAGTTTATTGAAATTC
AGGGTACTGCGGAAGAAAAACCGTTTACCCGTACCCAAGCCAATGCCATGCTTGAAGTGGCTGAAAAAGGCATTCAGGAA
CTCATTAAGAAACAACAGCAAGCATTGGGCTGGTAA

Upstream 100 bases:

>100_bases
AAAATGAAAACGATCTTCAGCTTGAGCAAATAAGGCTTTTGTAAAAAGCACAGAATTGGGCCGAATTCGGCCCAATTTTA
TTGGAGTCATTTGTTATACT

Downstream 100 bases:

>100_bases
GGTTCTTTTAAAATCAAAACGCATCTTTGGATGCGTTTTTTTATTTTTATAGTGACTGGACAAATAATTATTGTAGAAAT
ATTAGCATCTCTAATACTAT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLQSYLAAMKGWFMRLDQRALDQLRDVKITRNYTRYAEGSVLVEFGHTKVLCTASIDNSVPRFLKGQGQGWVTAEYGMLP
RSTHTRSDREAARGKQTGRTQEIQRLIGRSLRAMVDLKKLGENTITIDCDVIQADGGTRTAAITGAAVALIDAMNVLLEK
KKIKQDPLKGLVAAISVGIYQDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKGIQE
LIKKQQQALGW

Sequences:

>Translated_251_residues
MLQSYLAAMKGWFMRLDQRALDQLRDVKITRNYTRYAEGSVLVEFGHTKVLCTASIDNSVPRFLKGQGQGWVTAEYGMLP
RSTHTRSDREAARGKQTGRTQEIQRLIGRSLRAMVDLKKLGENTITIDCDVIQADGGTRTAAITGAAVALIDAMNVLLEK
KKIKQDPLKGLVAAISVGIYQDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKGIQE
LIKKQQQALGW
>Mature_251_residues
MLQSYLAAMKGWFMRLDQRALDQLRDVKITRNYTRYAEGSVLVEFGHTKVLCTASIDNSVPRFLKGQGQGWVTAEYGMLP
RSTHTRSDREAARGKQTGRTQEIQRLIGRSLRAMVDLKKLGENTITIDCDVIQADGGTRTAAITGAAVALIDAMNVLLEK
KKIKQDPLKGLVAAISVGIYQDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKGIQE
LIKKQQQALGW

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=69.6682464454976, Blast_Score=307, Evalue=5e-85,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=25.668449197861, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27898; Mature: 27898

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSYLAAMKGWFMRLDQRALDQLRDVKITRNYTRYAEGSVLVEFGHTKVLCTASIDNSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCEEEEEEECCCCCC
PRFLKGQGQGWVTAEYGMLPRSTHTRSDREAARGKQTGRTQEIQRLIGRSLRAMVDLKKL
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GENTITIDCDVIQADGGTRTAAITGAAVALIDAMNVLLEKKKIKQDPLKGLVAAISVGIY
CCCEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKGIQE
HHHHHHHHHHCCCCCCHHHHHHHEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LIKKQQQALGW
HHHHHHHHCCC
>Mature Secondary Structure
MLQSYLAAMKGWFMRLDQRALDQLRDVKITRNYTRYAEGSVLVEFGHTKVLCTASIDNSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCEEEEEEECCCCCC
PRFLKGQGQGWVTAEYGMLPRSTHTRSDREAARGKQTGRTQEIQRLIGRSLRAMVDLKKL
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GENTITIDCDVIQADGGTRTAAITGAAVALIDAMNVLLEKKKIKQDPLKGLVAAISVGIY
CCCEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKGIQE
HHHHHHHHHHCCCCCCHHHHHHHEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LIKKQQQALGW
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA