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The map label for this gene is rutF

Identifier: 50083325

GI number: 50083325

Start: 36303

End: 36869

Strand: Direct

Name: rutF

Synonym: ACIAD0030

Alternate gene names: 50083325

Gene position: 36303-36869 (Clockwise)

Preceding gene: 50083324

Following gene: 50083326

Centisome position: 1.01

GC content: 44.97

Gene sequence:

>567_bases
ATGACAGTATTAACACAAACTACAAAACACATAGAAAGTCTGCTCGGACAAATGAAACAAATTCATCGTGAAACTTTCAT
GCAGGTCGATCAGCAGGCTTTTCGTCAAGGGATGTCCAATTTAGGCGCTGCTGTCAATGTGATTACCACGGATGGCGTGG
CAGGAAAATCAGGCTTTACCGCATCTGCGGTGTGTAGTGTGACCGATACTCCGCCGACATTACTGGTCTGTCTAAATCGC
TCTGCCTCAGTATTTGAAACCTTTAAACAAAATCAGGTGTTGTGTGTCAATACACTGGCTGCACATCAGGCGCATCTCTC
TAATATTTTTGGTGGTAAAACCCCGATGGTAGACCGTTTTGCCCATGGTGAGTGGAGGACATTAGCCACACAGGCGCCTG
TACTTACAGATGCGCTGGTGTCTTTCGACTGTGAGGTTGTACAAAGCCTATCTGTTGGCAGTCATGATGTTTTTTTCTGT
CAGGTGAAGGCCATACAACATTGCCAAGGACAAAATGCCTTGATGTATTTTAACCGCAATTATTGCGAACCCCATTGTGT
AGCTTAA

Upstream 100 bases:

>100_bases
TGCCTCAGTTGCTCATGTTTAATCGACAGTCAAATTTATGACGTGAATAAATAGATAAATAAACCCCATCACCTTGAGTA
TTTTGGGGTGGAGATCTGCA

Downstream 100 bases:

>100_bases
GTCTCATATTGAAATGAGGAGAAATAGCGATGTCGAGTCAAGAACCGTGGTTTGCCAAATTTAAGCCTTATCAAGGCGAT
CTGGAACATCATCCTGTCCA

Product: flavin:NADH reductase (oxydoreductase)

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTVLTQTTKHIESLLGQMKQIHRETFMQVDQQAFRQGMSNLGAAVNVITTDGVAGKSGFTASAVCSVTDTPPTLLVCLNR
SASVFETFKQNQVLCVNTLAAHQAHLSNIFGGKTPMVDRFAHGEWRTLATQAPVLTDALVSFDCEVVQSLSVGSHDVFFC
QVKAIQHCQGQNALMYFNRNYCEPHCVA

Sequences:

>Translated_188_residues
MTVLTQTTKHIESLLGQMKQIHRETFMQVDQQAFRQGMSNLGAAVNVITTDGVAGKSGFTASAVCSVTDTPPTLLVCLNR
SASVFETFKQNQVLCVNTLAAHQAHLSNIFGGKTPMVDRFAHGEWRTLATQAPVLTDALVSFDCEVVQSLSVGSHDVFFC
QVKAIQHCQGQNALMYFNRNYCEPHCVA
>Mature_187_residues
TVLTQTTKHIESLLGQMKQIHRETFMQVDQQAFRQGMSNLGAAVNVITTDGVAGKSGFTASAVCSVTDTPPTLLVCLNRS
ASVFETFKQNQVLCVNTLAAHQAHLSNIFGGKTPMVDRFAHGEWRTLATQAPVLTDALVSFDCEVVQSLSVGSHDVFFCQ
VKAIQHCQGQNALMYFNRNYCEPHCVA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily

Homologues:

Organism=Escherichia coli, GI87081819, Length=153, Percent_Identity=62.0915032679739, Blast_Score=201, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTF_ACIAD (Q6FFZ4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044835.1
- ProteinModelPortal:   Q6FFZ4
- SMR:   Q6FFZ4
- STRING:   Q6FFZ4
- GeneID:   2878901
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0030
- NMPDR:   fig|62977.3.peg.1095
- eggNOG:   COG1853
- HOGENOM:   HBG730363
- OMA:   NRSASVY
- PhylomeDB:   Q6FFZ4
- BioCyc:   ASP62977:ACIAD0030-MONOMER
- HAMAP:   MF_00833
- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903
- TIGRFAMs:   TIGR03615

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: =1.5.1.29

Molecular weight: Translated: 20554; Mature: 20423

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLTQTTKHIESLLGQMKQIHRETFMQVDQQAFRQGMSNLGAAVNVITTDGVAGKSGFT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
ASAVCSVTDTPPTLLVCLNRSASVFETFKQNQVLCVNTLAAHQAHLSNIFGGKTPMVDRF
HHEEEEECCCCCEEEEEECCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHH
AHGEWRTLATQAPVLTDALVSFDCEVVQSLSVGSHDVFFCQVKAIQHCQGQNALMYFNRN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCCEEEEEECC
YCEPHCVA
CCCCCCCC
>Mature Secondary Structure 
TVLTQTTKHIESLLGQMKQIHRETFMQVDQQAFRQGMSNLGAAVNVITTDGVAGKSGFT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
ASAVCSVTDTPPTLLVCLNRSASVFETFKQNQVLCVNTLAAHQAHLSNIFGGKTPMVDRF
HHEEEEECCCCCEEEEEECCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHH
AHGEWRTLATQAPVLTDALVSFDCEVVQSLSVGSHDVFFCQVKAIQHCQGQNALMYFNRN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCCEEEEEECC
YCEPHCVA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA