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The map label for this gene is nemA [H]

Identifier: 49481767

GI number: 49481767

Start: 3477824

End: 3478951

Strand: Reverse

Name: nemA [H]

Synonym: BT9727_3398

Alternate gene names: 49481767

Gene position: 3478951-3477824 (Counterclockwise)

Preceding gene: 49478306

Following gene: 49481783

Centisome position: 66.42

GC content: 39.18

Gene sequence:

>1128_bases
ATGACAAGTTCAAACAAGAAATCGGCAAGTATTTATGAAGGAACAAATGTAAACGCTTGGGGGTCTTTTGAAAACGTTGC
AGAAACGAAGTTGTTTAATTCGATTCAAATTGGATCTTGGCCTCTTCGTAACCGTATAGCAATGGCACCAATGACAAGAT
GCTTTGCAGACAATGAAACTGGAGCAGTGGGTGCTGATGTAGTGGAATACTATAGAAAACGTGCAGCTGATGGAGTTGGA
TTGATTATTACAGAGGGAATCGTCATTAGCCCAAGAGCAAAAGGAAACCCAGGGGTACCGGGGATTTATACACAAGAACA
AATTGATTCGTGGAAACCTGTTACAGAGGCAGTACATAAAGAAGGCGGGACAATTATTGCTCAAATATGGCATGTTGGTC
GTATGAGTCATCATGAAATAATTGGTGGGCAAATGCCGCAAGCGCCGTCTGCAATCGCTGCAGAGGGAAATGTTCCACGT
TTTCGTAAGCCATTCGATACACCAGAAGCAATGACGTTAGAAGAAATACAAGAAGTTATCGGTCAATACGCACAAGCTGC
AAAAAATGCGATTGAAGCTGGATTTGATGGAGTAGAAATTCACGGTGCACACGGATATTTAATTGATCAATTTACTTATG
AGTTTGCAAACAAGCGTACAGATAAATATGGTGGCGACTTAAAGCAAAGGTTAACGTTTATGAAAGAAGTAACTGAGGCT
GTAATAGAAGCAATTGGAGCTGATAAAACACTTCTTCGTTTCTCTGCTTTTAAAGGTGATAATCCTACTTATATGTGGGA
AAACCCTGAATTGGCAATTGAAACATTTGTAAATATGTTTAAAGAAGTTGGATTAACGATGATTCATCCTTCAACGATGA
ATTATACGCAAGTGATTGCTGACGGTAAGAATTTTCATCAATTAGTAAGAAAATATTGGGATGGTGCGATTGTTGGGGTT
GGTAATTTGAATCCGAAAGAGGCAGAAGAAGCGTTGCAAGAAGGAACAATTGATGTAGCTGCATTCGGAAGACCTTTAAT
TGCTAATCCGGATTTTGTTCATCGAATTAAAAGTGCTGAAAGTTTAGTTGAATATGACGCGAAAGAGCATCTTGCTACAT
TGATTTGA

Upstream 100 bases:

>100_bases
AATATTCGAATAACCTCTGGTTAAAGCGTATAAATTGACGTTACTATCTAAAAGTAAGTATGATGCGATTGTAGTAAGAA
TTTTTTTAGGGGGAAAAAAC

Downstream 100 bases:

>100_bases
ATAAAGATAAAAGGAGAACACATGGAGTGTTCTCCTTTTATTATGGGATTTCATAGGGGGAAGCGAGATGAGTGAAACGC
TACTCGTTATTATTAGTATA

Product: Oye family NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVG
LIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIADGKNFHQLVRKYWDGAIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Sequences:

>Translated_375_residues
MTSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVG
LIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIADGKNFHQLVRKYWDGAIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI
>Mature_374_residues
TSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVGL
IITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRF
RKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEAV
IEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIADGKNFHQLVRKYWDGAIVGVG
NLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=345, Percent_Identity=38.2608695652174, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=31.304347826087, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17565138, Length=349, Percent_Identity=28.9398280802292, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=358, Percent_Identity=24.3016759776536, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=326, Percent_Identity=26.0736196319018, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=310, Percent_Identity=25.1612903225806, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=317, Percent_Identity=24.9211356466877, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=339, Percent_Identity=25.3687315634218, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=29.3023255813954, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=363, Percent_Identity=31.9559228650138, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325086, Length=362, Percent_Identity=31.2154696132597, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41367; Mature: 41236

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNET
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHCCCCC
GAVGADVVEYYRKRAADGVGLIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
DGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAE
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNET
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHCCCCC
GAVGADVVEYYRKRAADGVGLIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
DGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAE
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]