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The map label for this gene is ldh

Identifier: 49481755

GI number: 49481755

Start: 4757215

End: 4758420

Strand: Reverse

Name: ldh

Synonym: BT9727_4708

Alternate gene names: 49481755

Gene position: 4758420-4757215 (Counterclockwise)

Preceding gene: 49481053

Following gene: 49480763

Centisome position: 90.85

GC content: 34.16

Gene sequence:

>1206_bases
GTGCGGAATAACAAAAAAAGAAAACGAGCGAATCTATTCGCTCGTTTTCTTTTTGTATATGTCAATAAATACATAATAAA
TATTTTTCCAGTTGTAACAAATATGACGAAAATGGAATATAACAATTCACAAAAAGGACAAAAACTTTGCTCAATATTTC
ACAAAGTATCCATGGTTTCTATCGTGGGAACCTCTATAATTAGGAGTGTAAGGAACAAAAGAAATAGATTATATGATCAA
TTAGGAGAGATTGCTATGAAAAGACATACAAGAAAAATTGCAATTATCGGTACTGGATTAGTTGGATCAAGTTGTGCATA
TTCCATTGTCAATCAAGGCATTTGCGAAGAGCTATTATTAATTGATATAAATCATGAACGTGCAGTTGGGGAAGCAATGG
ATTTATCACACTGCATTAACTTTACAAATACAAGAACAAAAGTATATGCAGGAAGCTATGAAGACTGCAAAGATATGGAC
ATCGTTATTATTACAGCAGGACCAGCACCAAAGCCAGGACAAAGTCGCTTAGATACTTTAGGAGCGAGTGCAAAGATTAT
GGAAAGTGTTGTTGGCGGCGTAATGGAAAGTGGATTTGATGGTATTTTCTTACTTGCATCAAACCCAGTTGATATTATTA
CATATCAAGTGTGGAAATTATCTGGATTACCTAGAAATCGTGTGATCGGTACTGGTACATCACTAGATTCTTCTCGCTTA
CGAACAATTTTATCTGAAATGTTACATGTAGATCCTCGTAGTATTCATGGGTATTCATTAGGAGAACATGGTGATTCTCA
AATGGTTGCTTGGTCACACGTAACTGTTGGTGGAAAACCAATTCTGCAAATATTAGAAGAACAAAAAGAACGATTTGGTG
AAATAGATTTAGATGAAATCGTTGAGAAGACTGCAAAAGCCGGATGGGAAATTTATAAACGAAAAGGTACTACTTATTAC
GGAATCGGAAATTCTCTAGCATATATTGCAAACTCAATCTTTAACGATGATCACCGTGTAATTGCTGTATCAGCCATTTT
AGATGGTGAGTATGGTGAATATGATATTTGTACAGGAGTACCAGCTATTATTACTAGAGATGGTATAAGAGAAATTGTAG
AACTAAATTTAACAGAGGATGAAGAATCTCGATTCGCAAAATCAAACGATATTTTACGTGATTATATGAAAACAATTGGT
TACTAA

Upstream 100 bases:

>100_bases
CGCCAACGTAATATGTATGAGTATGCAAAGCAAAAGAGTTATTACAAACCGGCAGCACCTGCAAATCCGATGATTGTACA
GCAATTAAAAAGCCAATTAA

Downstream 100 bases:

>100_bases
CTTATAGGAGAAGGTGAAACAAATGAAGGAATATATAATGTCTCGTGTATTTAAAGCATCAGCTGGAATCGCACAAGGTA
TTTTCGTATCCCTTGGAATT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 3 [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRNNKKRKRANLFARFLFVYVNKYIINIFPVVTNMTKMEYNNSQKGQKLCSIFHKVSMVSIVGTSIIRSVRNKRNRLYDQ
LGEIAMKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMD
IVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRL
RTILSEMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYY
GIGNSLAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIG
Y

Sequences:

>Translated_401_residues
MRNNKKRKRANLFARFLFVYVNKYIINIFPVVTNMTKMEYNNSQKGQKLCSIFHKVSMVSIVGTSIIRSVRNKRNRLYDQ
LGEIAMKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMD
IVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRL
RTILSEMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYY
GIGNSLAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIG
Y
>Mature_401_residues
MRNNKKRKRANLFARFLFVYVNKYIINIFPVVTNMTKMEYNNSQKGQKLCSIFHKVSMVSIVGTSIIRSVRNKRNRLYDQ
LGEIAMKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMD
IVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRL
RTILSEMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYY
GIGNSLAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIG
Y

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=37.3417721518987, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI260099723, Length=335, Percent_Identity=36.4179104477612, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=36.8589743589744, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=36.8589743589744, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI291575128, Length=327, Percent_Identity=33.9449541284404, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4557032, Length=327, Percent_Identity=33.9449541284404, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI9257228, Length=314, Percent_Identity=36.9426751592357, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI4504973, Length=314, Percent_Identity=36.9426751592357, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=34.5276872964169, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI260099727, Length=218, Percent_Identity=38.5321100917431, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI260099725, Length=206, Percent_Identity=40.2912621359223, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI207028494, Length=234, Percent_Identity=36.7521367521368, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI103472011, Length=294, Percent_Identity=28.9115646258503, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=31.8918918918919, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI21735621, Length=254, Percent_Identity=28.3464566929134, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=36.241610738255, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6322765, Length=267, Percent_Identity=27.3408239700375, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136226, Length=335, Percent_Identity=35.5223880597015, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45550422, Length=308, Percent_Identity=30.1948051948052, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24663599, Length=339, Percent_Identity=25.6637168141593, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=25.3164556962025, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 44825; Mature: 44825

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNKKRKRANLFARFLFVYVNKYIINIFPVVTNMTKMEYNNSQKGQKLCSIFHKVSMVS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IVGTSIIRSVRNKRNRLYDQLGEIAMKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEE
IDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTL
EECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHH
GASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRL
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHH
RTILSEMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEI
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHH
VEKTAKAGWEIYKRKGTTYYGIGNSLAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGV
HHHHHHCCHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEHHCCC
PAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNNKKRKRANLFARFLFVYVNKYIINIFPVVTNMTKMEYNNSQKGQKLCSIFHKVSMVS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IVGTSIIRSVRNKRNRLYDQLGEIAMKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEE
IDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTL
EECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHH
GASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRL
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHH
RTILSEMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEI
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHH
VEKTAKAGWEIYKRKGTTYYGIGNSLAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGV
HHHHHHCCHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEHHCCC
PAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]