Click here to switch to the map view.

The map label for this gene is leuS

Identifier: 49481694

GI number: 49481694

Start: 4525338

End: 4527746

Strand: Reverse

Name: leuS

Synonym: BT9727_4472

Alternate gene names: 49481694

Gene position: 4527746-4525338 (Counterclockwise)

Preceding gene: 49481565

Following gene: 49480717

Centisome position: 86.45

GC content: 41.14

Gene sequence:

>2409_bases
ATGAGCTTTAATCATCAAGAAATTGAGAAGAAGTGGCAAGGGTACTGGGAAGAGAATAAAACATTCCGTACGCCAGATGA
GACAGAAAAACCAAAATTTTATGCACTAGATATGTTCCCATATCCATCAGGTGCAGGCTTACACGTAGGACATCCAGAAG
GTTATACAGCAACGGATATTTTATCTCGTATGAAGCGTATGCAAGGGTATAACGTTCTTCATCCAATGGGATGGGATGCA
TTCGGTCTTCCAGCAGAGCAATATGCACTTGATACTGGAAACAGCCCAGCTGAATTTACAGAGCATAATATTAATACATT
CCGTAATCAAATTAAATCATTAGGTTTCTCTTACGATTGGGACCGTGAAGTAAATACAACAGATCCAAACTACTACAAGT
GGACACAATGGATCTTCCTAAAACTATTTGAAAAAGGCTTAGCTTACGTTGATGAAGTACCTGTAAACTGGTGCCCAGCA
CTTGGTACAGTACTTGCAAACGAAGAAATCATTGACGGTAAGAGTGAGCGCGGTGGACATCCAGTTGAGCGTCGTCCGAT
GAGACAGTGGATGTTAAAAATTACAGCTTACGGAGATCGTCTATTAGAAGATCTAGACGAGCTTGATTGGCCAGAAAGCT
TAAAAGATATGCAACGTAACTGGATCGGTCGTTCTGAAGGTGCAGAAGTACACTTCAACATCGACGGTACAGACGAGAAG
TTCACAGTTTTCACAACGCGCCCTGATACACTATTTGGTGCAAGCTACTGTGTACTTGCTCCGGAACATGCGCTTGTTGC
TGATATTACAACTGCTGAACAAAAAGAAGCTGTAGAAGCTTACATTAATTCTGTAAAAATGAAGAGTGATTTAGAGCGTA
CAGAACTTGCGAAAGAGAAAACTGGTGTATTCACTGGTGCTTACGCAGTTAACCCAGTAAATGGTGAGAAATTACCAATC
TGGATCGCTGATTACGTTCTTGCAACTTACGGAACAGGTGCTGTAATGGCAGTTCCAGCTCATGATGAGCGTGACTATGA
ATTCGCATCAACGTTCAATCTTCCAATGAAGGAAGTTGTAAAAGGCGGAGACATTACGAAAGAAGCATACACAGGTGATG
GTGCTCACGTAAACTCAGCATTCCTTGATGGCTTAAATAAAGAAGAAGCAATCGCAAAAATGATTGAATGGCTTGAAGTA
ACGAGCGCAGGAAATCAAAAAGTAACATACCGTCTACGTGACTGGTTATTCAGCCGTCAACGTTACTGGGGTGAGCCAAT
TCCAGTTATTCACTGGGAAGATGGCACAATGACAGCCGTGAAAGAAGAAGAATTACCATTAGTTCTTCCGAAAACAGAAA
ACATCCGTCCTTCAGGTACAGGTGAATCACCACTTGCTAACATTGACGAGTGGGTAAATGTTGTTGATCCTGAGACTGGT
AAAAAAGGTCGTCGTGAAACAAATACAATGCCGCAATGGGCTGGTAGCTGCTGGTACTACCTACGTTACATCGATCCAAA
CAACAGCGAAGCACTTGTAGATCCTGAAAAAGTAAAACAGTGGCTTCCAGTTGATATTTATATCGGTGGTGCAGAACACG
CTGTACTTCACTTACTATATGCTCGTTTCTGGCACAAAGTATTATACGATATCGGTGTAGTTCCAACGAAAGAGCCGTTC
CAACAATTATTCAACCAAGGTATGATTCTAGGTGAAAACAACGAGAAAATGAGTAAATCAAAAGGTAACGTTGTAAACCC
TGATGATATCGTAGCAAGCCACGGTGCAGATACACTTCGTCTATACGAAATGTTCATGGGACCATTAGATGCTTCAATTG
CTTGGTCTGAAAATGGTCTTGACGGAGCTCGTCGTTTCCTAGACCGCGTATGGCGCCTATTCGTTCAAGATAACGGTGAA
TTAAGTGAGAAAATTACTGATGCACCAAATAAAGATCTTGAAAAAGCTTACCACCAAACAGTGAAGAAAGTAACAGAAGA
CTATGCAGAGCTTCGCTTCAACACAGCGATTTCTCAAATGATGGTATTCATCAACGATGCATACAAAGCTGAAACACTTC
CGAAAGAATATGTAGAAGGTTTCGTAAAAATGATTGCACCAGTTGCACCTCACATCGGGGAAGAGCTATGGAGCAAACTT
GGATACAATGAAACAATCACATATGCAAGCTGGCCAACATTCGATGAGTCTAAACTTGTAGAAGATGAAGTTGAAATCGT
TGTTCAAGTTATGGGTAAAGTTCGCGCAAAACTAACAATGAGTAAAGACGCATCAAAAGACGAAATGGAAAAACTTGCAC
TTGAAGCAATTCAAGACCAAATCGAAGGAAAAACAGTTCGTAAAGTAATTGTAGTTCCTGGAAAACTTGTTAACGTTGTT
GCAAACTAA

Upstream 100 bases:

>100_bases
GGTACCGCGGGAATCTATAACCTCTCGTCCCTTTCTAGGGATGAGAGGTTTTTTGTATTTTGGGCGGTGAAAATAAATAG
AATTTTAAGGAGGGTATCTC

Downstream 100 bases:

>100_bases
TTTATTCTAAATAAAAGCTCAGAGTATTTATGCTCTGAGCTTTTTTGTGTAGAAAATAATTTCTGGATCAGCTTCATCTA
AATTTTCAACTATTCCACTA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MSFNHQEIEKKWQGYWEENKTFRTPDETEKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNSPAEFTEHNINTFRNQIKSLGFSYDWDREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEVPVNWCPA
LGTVLANEEIIDGKSERGGHPVERRPMRQWMLKITAYGDRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDEK
FTVFTTRPDTLFGASYCVLAPEHALVADITTAEQKEAVEAYINSVKMKSDLERTELAKEKTGVFTGAYAVNPVNGEKLPI
WIADYVLATYGTGAVMAVPAHDERDYEFASTFNLPMKEVVKGGDITKEAYTGDGAHVNSAFLDGLNKEEAIAKMIEWLEV
TSAGNQKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTMTAVKEEELPLVLPKTENIRPSGTGESPLANIDEWVNVVDPETG
KKGRRETNTMPQWAGSCWYYLRYIDPNNSEALVDPEKVKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPF
QQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLRLYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQDNGE
LSEKITDAPNKDLEKAYHQTVKKVTEDYAELRFNTAISQMMVFINDAYKAETLPKEYVEGFVKMIAPVAPHIGEELWSKL
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLTMSKDASKDEMEKLALEAIQDQIEGKTVRKVIVVPGKLVNVV
AN

Sequences:

>Translated_802_residues
MSFNHQEIEKKWQGYWEENKTFRTPDETEKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNSPAEFTEHNINTFRNQIKSLGFSYDWDREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEVPVNWCPA
LGTVLANEEIIDGKSERGGHPVERRPMRQWMLKITAYGDRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDEK
FTVFTTRPDTLFGASYCVLAPEHALVADITTAEQKEAVEAYINSVKMKSDLERTELAKEKTGVFTGAYAVNPVNGEKLPI
WIADYVLATYGTGAVMAVPAHDERDYEFASTFNLPMKEVVKGGDITKEAYTGDGAHVNSAFLDGLNKEEAIAKMIEWLEV
TSAGNQKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTMTAVKEEELPLVLPKTENIRPSGTGESPLANIDEWVNVVDPETG
KKGRRETNTMPQWAGSCWYYLRYIDPNNSEALVDPEKVKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPF
QQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLRLYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQDNGE
LSEKITDAPNKDLEKAYHQTVKKVTEDYAELRFNTAISQMMVFINDAYKAETLPKEYVEGFVKMIAPVAPHIGEELWSKL
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLTMSKDASKDEMEKLALEAIQDQIEGKTVRKVIVVPGKLVNVV
AN
>Mature_801_residues
SFNHQEIEKKWQGYWEENKTFRTPDETEKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYNVLHPMGWDAF
GLPAEQYALDTGNSPAEFTEHNINTFRNQIKSLGFSYDWDREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEVPVNWCPAL
GTVLANEEIIDGKSERGGHPVERRPMRQWMLKITAYGDRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDEKF
TVFTTRPDTLFGASYCVLAPEHALVADITTAEQKEAVEAYINSVKMKSDLERTELAKEKTGVFTGAYAVNPVNGEKLPIW
IADYVLATYGTGAVMAVPAHDERDYEFASTFNLPMKEVVKGGDITKEAYTGDGAHVNSAFLDGLNKEEAIAKMIEWLEVT
SAGNQKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTMTAVKEEELPLVLPKTENIRPSGTGESPLANIDEWVNVVDPETGK
KGRRETNTMPQWAGSCWYYLRYIDPNNSEALVDPEKVKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPFQ
QLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLRLYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQDNGEL
SEKITDAPNKDLEKAYHQTVKKVTEDYAELRFNTAISQMMVFINDAYKAETLPKEYVEGFVKMIAPVAPHIGEELWSKLG
YNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLTMSKDASKDEMEKLALEAIQDQIEGKTVRKVIVVPGKLVNVVA
N

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=875, Percent_Identity=32.1142857142857, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI94721239, Length=688, Percent_Identity=23.1104651162791, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI94721241, Length=688, Percent_Identity=23.1104651162791, Blast_Score=94, Evalue=6e-19,
Organism=Escherichia coli, GI1786861, Length=863, Percent_Identity=43.4530706836616, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=391, Percent_Identity=24.5524296675192, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71997510, Length=807, Percent_Identity=29.4919454770756, Blast_Score=311, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI71997517, Length=775, Percent_Identity=30.0645161290323, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI212645227, Length=366, Percent_Identity=28.6885245901639, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17510661, Length=378, Percent_Identity=24.3386243386243, Blast_Score=97, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71980946, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=823, Percent_Identity=36.81652490887, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321531, Length=378, Percent_Identity=25.1322751322751, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319395, Length=373, Percent_Identity=23.8605898123324, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355409, Length=819, Percent_Identity=31.990231990232, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281366294, Length=364, Percent_Identity=26.0989010989011, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91288; Mature: 91157

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNHQEIEKKWQGYWEENKTFRTPDETEKPKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMKRMQGYNVLHPMGWDAFGLPAEQYALDTGNSPAEFTEHNINTFRNQIKSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEVPVNWCPALGTVLANEEIIDGKSERGGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMRQWMLKITAYGDRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
FTVFTTRPDTLFGASYCVLAPEHALVADITTAEQKEAVEAYINSVKMKSDLERTELAKEK
EEEEECCCCCHHCCEEEEECCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVFTGAYAVNPVNGEKLPIWIADYVLATYGTGAVMAVPAHDERDYEFASTFNLPMKEVV
CCEEECCEEECCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCHHHHH
KGGDITKEAYTGDGAHVNSAFLDGLNKEEAIAKMIEWLEVTSAGNQKVTYRLRDWLFSRQ
CCCCCCHHHCCCCCCEECHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
RYWGEPIPVIHWEDGTMTAVKEEELPLVLPKTENIRPSGTGESPLANIDEWVNVVDPETG
HHCCCCCCEEEECCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
KKGRRETNTMPQWAGSCWYYLRYIDPNNSEALVDPEKVKQWLPVDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHH
ARFWHKVLYDIGVVPTKEPFQQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHCCCCCCCHHHHHHCCCCHHHH
LYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQDNGELSEKITDAPNKDLEKAYHQT
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHH
VKKVTEDYAELRFNTAISQMMVFINDAYKAETLPKEYVEGFVKMIAPVAPHIGEELWSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLTMSKDASKDEMEKLALEAIQDQ
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHH
IEGKTVRKVIVVPGKLVNVVAN
HCCCEEEEEEECCCHHHHHHCC
>Mature Secondary Structure 
SFNHQEIEKKWQGYWEENKTFRTPDETEKPKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMKRMQGYNVLHPMGWDAFGLPAEQYALDTGNSPAEFTEHNINTFRNQIKSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEVPVNWCPALGTVLANEEIIDGKSERGGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMRQWMLKITAYGDRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
FTVFTTRPDTLFGASYCVLAPEHALVADITTAEQKEAVEAYINSVKMKSDLERTELAKEK
EEEEECCCCCHHCCEEEEECCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVFTGAYAVNPVNGEKLPIWIADYVLATYGTGAVMAVPAHDERDYEFASTFNLPMKEVV
CCEEECCEEECCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCHHHHH
KGGDITKEAYTGDGAHVNSAFLDGLNKEEAIAKMIEWLEVTSAGNQKVTYRLRDWLFSRQ
CCCCCCHHHCCCCCCEECHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
RYWGEPIPVIHWEDGTMTAVKEEELPLVLPKTENIRPSGTGESPLANIDEWVNVVDPETG
HHCCCCCCEEEECCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
KKGRRETNTMPQWAGSCWYYLRYIDPNNSEALVDPEKVKQWLPVDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHH
ARFWHKVLYDIGVVPTKEPFQQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHCCCCCCCHHHHHHCCCCHHHH
LYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQDNGELSEKITDAPNKDLEKAYHQT
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHH
VKKVTEDYAELRFNTAISQMMVFINDAYKAETLPKEYVEGFVKMIAPVAPHIGEELWSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLTMSKDASKDEMEKLALEAIQDQ
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHH
IEGKTVRKVIVVPGKLVNVVAN
HCCCEEEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA