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The map label for this gene is asnC

Identifier: 49481692

GI number: 49481692

Start: 4356847

End: 4358238

Strand: Reverse

Name: asnC

Synonym: BT9727_4292

Alternate gene names: 49481692

Gene position: 4358238-4356847 (Counterclockwise)

Preceding gene: 49478689

Following gene: 49481453

Centisome position: 83.21

GC content: 40.88

Gene sequence:

>1392_bases
ATGGAAAACACATTAGTAAAAAGTCTGTATAGAGATACAGATAAATACGCAGGTCAAACAGTACAAGTATCAGGGTGGAT
TCGTAACTTACGTGATTCAAAAGCATTTGGTTTCATCGAATTAAACGACGGTAGCTTCTTCAAAAGCGTTCAAATCGTTT
TCGATACAGAATTAGATAACTTCAAAGAAATTGCAAAACTTCCACTTAGCTCTTCAGTTAAAGTAGAAGGTAAAGTAATT
GCAACGCCTGGAGCGAAGCAACCATTTGAAATTAAAGCAGAAAAAATTGATATCGAGGGCTTATCAGATTCTGATTACCC
ACTTCAAAAGAAACGTCATACGTTTGAATACTTACGTACAATCGCTCACTTACGTCCAAGAACAAATGCATTCTCTGCAA
CGTTCCGCGTACGTTCTATCGCAGCATTTGCGATTCACCAATTCTTCCAAGAGCGTGGATTCGTACATGTTCACACACCA
ATCATCACTGGTAGTGATACAGAAGGTGCGGGCGAAATGTTCCGCGTAACAACGCAAGATTTAAACAACGTACCAAAAGA
CGAAGACGGACAAGTGGATGAATCAAAAGACTTCTTCGGCAAAGAAACAAACTTAACAGTAAGTGGACAGCTGAACGCTG
AAGCTTATGCATTAGCATTCCGTGATGTATACACATTCGGACCTACATTCCGTGCAGAAAACTCAAACACAACTCGCCAC
GCAGCTGAGTTCTGGATGGTTGAGCCTGAGATTGCATTTGCTGAACTAGGTGACGTAATGAACCTTACAGAAGATATGCT
GAAATATGCAATGAAATACGTATTAGAGCATGCACCAGAAGAAATGGAATTCTTCAACAGCTTCGTTGATAAAACAGTTC
TAGAGCGCATGAACAACGTAATCAACTCTGACTTCGGTCGCATCACTTATACAGAAGCAATTAAAGTACTTCAAGAGTCA
GGTGCTGACTTCAAATACCCAGTAGAATGGGGTATTGACTTACAAACAGAGCACGAAAGATACTTATCAGAAGAAATCTT
CAAACGCCCTGTATTCGTAACTGACTATCCGAAAGACATTAAAGCATTCTACATGCGCTTAAATGACGACGGTAAAACAG
TAGCTGCTACTGACCTTCTAGTTCCTGGCATCGGCGAATTAATCGGCGGAAGCCAACGTGAAGAAAGAATGGACGTTTTA
GTAGACAGAATTAAAGAACTAGGCATGAACGAAGAGGACTACTGGTGGTACTTAGAACTTAGAAAATACGGCGGTACAAA
ACACGCTGGATTCGGTCTAGGTTTCGAGCGCTTCCTAATGTATATCACTGGCATGGCTAACATCCGTGACGTAATTCCAT
TCCCAAGAACTCCAGGTTCTTCTGAATTCTAA

Upstream 100 bases:

>100_bases
TTATGACAAATTGGGTGGTACCGCGAAGTTTAACCTTTCGTCCCTTTATGGATGAAAGGTTTTTTTGTATTTAAATATAT
CAGCAAAGGAGAATTTCACT

Downstream 100 bases:

>100_bases
AATATGAAAAAGCGAGAGGGTTTTCTCTCGCTTTTTTATTTTGTATAGTTTACTACCTCGTAGCAAAAAGTAACGCGAAG
GGGTGAATATAAATGAGTAA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MENTLVKSLYRDTDKYAGQTVQVSGWIRNLRDSKAFGFIELNDGSFFKSVQIVFDTELDNFKEIAKLPLSSSVKVEGKVI
ATPGAKQPFEIKAEKIDIEGLSDSDYPLQKKRHTFEYLRTIAHLRPRTNAFSATFRVRSIAAFAIHQFFQERGFVHVHTP
IITGSDTEGAGEMFRVTTQDLNNVPKDEDGQVDESKDFFGKETNLTVSGQLNAEAYALAFRDVYTFGPTFRAENSNTTRH
AAEFWMVEPEIAFAELGDVMNLTEDMLKYAMKYVLEHAPEEMEFFNSFVDKTVLERMNNVINSDFGRITYTEAIKVLQES
GADFKYPVEWGIDLQTEHERYLSEEIFKRPVFVTDYPKDIKAFYMRLNDDGKTVAATDLLVPGIGELIGGSQREERMDVL
VDRIKELGMNEEDYWWYLELRKYGGTKHAGFGLGFERFLMYITGMANIRDVIPFPRTPGSSEF

Sequences:

>Translated_463_residues
MENTLVKSLYRDTDKYAGQTVQVSGWIRNLRDSKAFGFIELNDGSFFKSVQIVFDTELDNFKEIAKLPLSSSVKVEGKVI
ATPGAKQPFEIKAEKIDIEGLSDSDYPLQKKRHTFEYLRTIAHLRPRTNAFSATFRVRSIAAFAIHQFFQERGFVHVHTP
IITGSDTEGAGEMFRVTTQDLNNVPKDEDGQVDESKDFFGKETNLTVSGQLNAEAYALAFRDVYTFGPTFRAENSNTTRH
AAEFWMVEPEIAFAELGDVMNLTEDMLKYAMKYVLEHAPEEMEFFNSFVDKTVLERMNNVINSDFGRITYTEAIKVLQES
GADFKYPVEWGIDLQTEHERYLSEEIFKRPVFVTDYPKDIKAFYMRLNDDGKTVAATDLLVPGIGELIGGSQREERMDVL
VDRIKELGMNEEDYWWYLELRKYGGTKHAGFGLGFERFLMYITGMANIRDVIPFPRTPGSSEF
>Mature_463_residues
MENTLVKSLYRDTDKYAGQTVQVSGWIRNLRDSKAFGFIELNDGSFFKSVQIVFDTELDNFKEIAKLPLSSSVKVEGKVI
ATPGAKQPFEIKAEKIDIEGLSDSDYPLQKKRHTFEYLRTIAHLRPRTNAFSATFRVRSIAAFAIHQFFQERGFVHVHTP
IITGSDTEGAGEMFRVTTQDLNNVPKDEDGQVDESKDFFGKETNLTVSGQLNAEAYALAFRDVYTFGPTFRAENSNTTRH
AAEFWMVEPEIAFAELGDVMNLTEDMLKYAMKYVLEHAPEEMEFFNSFVDKTVLERMNNVINSDFGRITYTEAIKVLQES
GADFKYPVEWGIDLQTEHERYLSEEIFKRPVFVTDYPKDIKAFYMRLNDDGKTVAATDLLVPGIGELIGGSQREERMDVL
VDRIKELGMNEEDYWWYLELRKYGGTKHAGFGLGFERFLMYITGMANIRDVIPFPRTPGSSEF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=450, Percent_Identity=46.2222222222222, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4758762, Length=466, Percent_Identity=29.6137339055794, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI45439306, Length=341, Percent_Identity=28.4457478005865, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787161, Length=447, Percent_Identity=59.0604026845638, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1790571, Length=377, Percent_Identity=28.3819628647215, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789256, Length=374, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788173, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556372, Length=428, Percent_Identity=32.4766355140187, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI71984122, Length=460, Percent_Identity=30, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17551876, Length=349, Percent_Identity=28.6532951289398, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319872, Length=484, Percent_Identity=39.4628099173554, Blast_Score=328, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6321807, Length=474, Percent_Identity=29.957805907173, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6323011, Length=346, Percent_Identity=29.1907514450867, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922416, Length=458, Percent_Identity=36.0262008733624, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI19921528, Length=454, Percent_Identity=28.8546255506608, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17136276, Length=480, Percent_Identity=25.2083333333333, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 52984; Mature: 52984

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTLVKSLYRDTDKYAGQTVQVSGWIRNLRDSKAFGFIELNDGSFFKSVQIVFDTELDN
CCHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCEEEEEECCCCEEEEEEEEEECCCHH
FKEIAKLPLSSSVKVEGKVIATPGAKQPFEIKAEKIDIEGLSDSDYPLQKKRHTFEYLRT
HHHHHHCCCCCCEEECCEEEECCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IAHLRPRTNAFSATFRVRSIAAFAIHQFFQERGFVHVHTPIITGSDTEGAGEMFRVTTQD
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEEEHHH
LNNVPKDEDGQVDESKDFFGKETNLTVSGQLNAEAYALAFRDVYTFGPTFRAENSNTTRH
HCCCCCCCCCCCCCCHHHCCCCCCEEEEECCCCCEEEEEHHHHHHCCCCEECCCCCCHHH
AAEFWMVEPEIAFAELGDVMNLTEDMLKYAMKYVLEHAPEEMEFFNSFVDKTVLERMNNV
HHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
INSDFGRITYTEAIKVLQESGADFKYPVEWGIDLQTEHERYLSEEIFKRPVFVTDYPKDI
HCCCCCCEEHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHHHCCCEEECCCHHH
KAFYMRLNDDGKTVAATDLLVPGIGELIGGSQREERMDVLVDRIKELGMNEEDYWWYLEL
EEEEEEECCCCCEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RKYGGTKHAGFGLGFERFLMYITGMANIRDVIPFPRTPGSSEF
EECCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MENTLVKSLYRDTDKYAGQTVQVSGWIRNLRDSKAFGFIELNDGSFFKSVQIVFDTELDN
CCHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCEEEEEECCCCEEEEEEEEEECCCHH
FKEIAKLPLSSSVKVEGKVIATPGAKQPFEIKAEKIDIEGLSDSDYPLQKKRHTFEYLRT
HHHHHHCCCCCCEEECCEEEECCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IAHLRPRTNAFSATFRVRSIAAFAIHQFFQERGFVHVHTPIITGSDTEGAGEMFRVTTQD
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEEEHHH
LNNVPKDEDGQVDESKDFFGKETNLTVSGQLNAEAYALAFRDVYTFGPTFRAENSNTTRH
HCCCCCCCCCCCCCCHHHCCCCCCEEEEECCCCCEEEEEHHHHHHCCCCEECCCCCCHHH
AAEFWMVEPEIAFAELGDVMNLTEDMLKYAMKYVLEHAPEEMEFFNSFVDKTVLERMNNV
HHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
INSDFGRITYTEAIKVLQESGADFKYPVEWGIDLQTEHERYLSEEIFKRPVFVTDYPKDI
HCCCCCCEEHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHHHCCCEEECCCHHH
KAFYMRLNDDGKTVAATDLLVPGIGELIGGSQREERMDVLVDRIKELGMNEEDYWWYLEL
EEEEEEECCCCCEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RKYGGTKHAGFGLGFERFLMYITGMANIRDVIPFPRTPGSSEF
EECCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]