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The map label for this gene is trpS

Identifier: 49481689

GI number: 49481689

Start: 3211281

End: 3212279

Strand: Reverse

Name: trpS

Synonym: BT9727_3143

Alternate gene names: 49481689

Gene position: 3212279-3211281 (Counterclockwise)

Preceding gene: 49479012

Following gene: 49478197

Centisome position: 61.33

GC content: 38.84

Gene sequence:

>999_bases
ATGAGTGACAAAATAATGTTAACAGGAATTAAGCCGACAGGTTATCCGCATTTAGGAAATTATATTGGTGCAATTAAACC
TGCGTTGCAAATGTCAAAGAAGAATGAAGGGAAAGCGTTGTATTTTATAGCAGATTATCACGCGTTAAATGCTGTGCATG
ATCCGGGTCAGTTTAGTAGTTATACGAAAGAGGTAGCTGCTACTTGGTTATCGCTTGGACTTGGAGAAGATGTTATATTT
TACCGACAAACAGAAGTGCCGGAGATTCTAGAATTAGCTTGGATATTAGCTTGCCTAACTCCGAAAGGGCTTATGAACCG
TGCTCATGCGTATAAAGCGAAGGTGGAGCAAAATAAAGAAGCGGGTTTAGAGGTCGATGCAGGAGTGAATATGGGATTAT
ATACGTATCCGATCTTAATGGCAGCTGACATATTGCTATTTCAGGCTACGCATGTGCCAGTCGGAAAAGACCAAATTCAG
CATATTGAAATTGCGCGTGATATTGCCACGTATTTTAACCATACATTCGGCATGACATTTACACTTCCAGAGTATGTCAT
ACAAGAAGAAGGCGCAATATTACCAGGGCTTGATGGAAGAAAGATGAGTAAAAGTTATGGGAATGTGATCCCATTATTTG
CAGAGCAAGAAAAACTACGAAAGTTAATATTTAAAATCAAAACCGATTCTTCACTTCCAAATGAACCGAAAGAATTAGAA
ACGTTATTTACGATTTATAAAGAATTTGCAACAGAGGATGAAATGCATTCGTTACGTGAAAAATATGAAACTGGCATTGG
ATGGGGGGATGTGAAAAAAGAGTTGTTTCGCGTTGTAGATCGTGAATTAGCTGGACCTCGTGAGAAATATGCAATGTATA
TGAATGAACCGAATTTATTATATGAAGCGTTGGAAAAAGGTGCTGAACGGACAAGAGAAATCGCGAAGGCAAATTTGGTG
GGAATTAAAAAACAGATTGGATTTGAGAGGGGACGCTGA

Upstream 100 bases:

>100_bases
TGGGCTATTCATGATAGCTAATTAAGGTGGTACCGCGGGAGTCTCTCGTCCTTTTTTAAGGATGAGGGGCTTTTTTTATT
TTGAGAAAGGAGGAGTTAGG

Downstream 100 bases:

>100_bases
GCGTTCCCTTGTTTTTTTACAAAAAAACGAACGGAATTTCAATTTGTTACCAATATAGAGTGAGGAGGGATAGAAATGAT
TACAGCTCTCGAGTTAAAAG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSDKIMLTGIKPTGYPHLGNYIGAIKPALQMSKKNEGKALYFIADYHALNAVHDPGQFSSYTKEVAATWLSLGLGEDVIF
YRQTEVPEILELAWILACLTPKGLMNRAHAYKAKVEQNKEAGLEVDAGVNMGLYTYPILMAADILLFQATHVPVGKDQIQ
HIEIARDIATYFNHTFGMTFTLPEYVIQEEGAILPGLDGRKMSKSYGNVIPLFAEQEKLRKLIFKIKTDSSLPNEPKELE
TLFTIYKEFATEDEMHSLREKYETGIGWGDVKKELFRVVDRELAGPREKYAMYMNEPNLLYEALEKGAERTREIAKANLV
GIKKQIGFERGR

Sequences:

>Translated_332_residues
MSDKIMLTGIKPTGYPHLGNYIGAIKPALQMSKKNEGKALYFIADYHALNAVHDPGQFSSYTKEVAATWLSLGLGEDVIF
YRQTEVPEILELAWILACLTPKGLMNRAHAYKAKVEQNKEAGLEVDAGVNMGLYTYPILMAADILLFQATHVPVGKDQIQ
HIEIARDIATYFNHTFGMTFTLPEYVIQEEGAILPGLDGRKMSKSYGNVIPLFAEQEKLRKLIFKIKTDSSLPNEPKELE
TLFTIYKEFATEDEMHSLREKYETGIGWGDVKKELFRVVDRELAGPREKYAMYMNEPNLLYEALEKGAERTREIAKANLV
GIKKQIGFERGR
>Mature_331_residues
SDKIMLTGIKPTGYPHLGNYIGAIKPALQMSKKNEGKALYFIADYHALNAVHDPGQFSSYTKEVAATWLSLGLGEDVIFY
RQTEVPEILELAWILACLTPKGLMNRAHAYKAKVEQNKEAGLEVDAGVNMGLYTYPILMAADILLFQATHVPVGKDQIQH
IEIARDIATYFNHTFGMTFTLPEYVIQEEGAILPGLDGRKMSKSYGNVIPLFAEQEKLRKLIFKIKTDSSLPNEPKELET
LFTIYKEFATEDEMHSLREKYETGIGWGDVKKELFRVVDRELAGPREKYAMYMNEPNLLYEALEKGAERTREIAKANLVG
IKKQIGFERGR

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=206, Percent_Identity=37.8640776699029, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI41352700, Length=187, Percent_Identity=38.5026737967914, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1789786, Length=341, Percent_Identity=32.5513196480938, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71982800, Length=339, Percent_Identity=31.5634218289086, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71982793, Length=339, Percent_Identity=31.5634218289086, Blast_Score=140, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320474, Length=349, Percent_Identity=34.0974212034384, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24666151, Length=335, Percent_Identity=30.1492537313433, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37548; Mature: 37417

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50237 HECT ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKIMLTGIKPTGYPHLGNYIGAIKPALQMSKKNEGKALYFIADYHALNAVHDPGQFSS
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCHHHH
YTKEVAATWLSLGLGEDVIFYRQTEVPEILELAWILACLTPKGLMNRAHAYKAKVEQNKE
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHH
AGLEVDAGVNMGLYTYPILMAADILLFQATHVPVGKDQIQHIEIARDIATYFNHTFGMTF
CCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
TLPEYVIQEEGAILPGLDGRKMSKSYGNVIPLFAEQEKLRKLIFKIKTDSSLPNEPKELE
ECHHHHHHCCCCCCCCCCCCHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
TLFTIYKEFATEDEMHSLREKYETGIGWGDVKKELFRVVDRELAGPREKYAMYMNEPNLL
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEECCCHHH
YEALEKGAERTREIAKANLVGIKKQIGFERGR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDKIMLTGIKPTGYPHLGNYIGAIKPALQMSKKNEGKALYFIADYHALNAVHDPGQFSS
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCHHHH
YTKEVAATWLSLGLGEDVIFYRQTEVPEILELAWILACLTPKGLMNRAHAYKAKVEQNKE
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHH
AGLEVDAGVNMGLYTYPILMAADILLFQATHVPVGKDQIQHIEIARDIATYFNHTFGMTF
CCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
TLPEYVIQEEGAILPGLDGRKMSKSYGNVIPLFAEQEKLRKLIFKIKTDSSLPNEPKELE
ECHHHHHHCCCCCCCCCCCCHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
TLFTIYKEFATEDEMHSLREKYETGIGWGDVKKELFRVVDRELAGPREKYAMYMNEPNLL
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEECCCHHH
YEALEKGAERTREIAKANLVGIKKQIGFERGR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]