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The map label for this gene is ruvB

Identifier: 49481506

GI number: 49481506

Start: 4216242

End: 4217252

Strand: Reverse

Name: ruvB

Synonym: BT9727_4153

Alternate gene names: 49481506

Gene position: 4217252-4216242 (Counterclockwise)

Preceding gene: 49481508

Following gene: 49480668

Centisome position: 80.52

GC content: 41.44

Gene sequence:

>1011_bases
GTGAGTATAATGGACGAACGTCTCCTTTCAGGGGAATCTGCATATGAAGATGCGGATTTAGAATATTCATTACGGCCACA
GACGCTCCGTCAGTATATTGGCCAAGATAAAGCGAAACATAATTTAGAAGTGTTTATTGAAGCGGCGAAAATGCGTGAAG
AAACGTTAGATCACGTGCTTTTATATGGACCACCAGGACTTGGTAAAACGACGCTTGCAAATATTATTGCCAATGAAATG
GGCGTAAATGTTAGAACAACTTCAGGTCCAGCAATCGAAAGGCCAGGAGATTTAGCAGCTGTATTAACATCGCTTCAACC
AGGGGATGTATTATTTATTGATGAAATTCATCGTTTGCATAGATCAATTGAAGAAGTACTATATCCTGCGATGGAAGATT
TTTGCCTTGATATTGTCATTGGAAAAGGACCGTCAGCGCGATCTGTACGTTTAGATTTACCGCCATTTACATTAGTTGGA
GCAACGACGCGTGCGGGAGCATTATCAGCGCCATTACGTGACCGTTTCGGTGTACTTTCAAGATTAGAGTATTACACAGT
AGATCAGCTTTCTGCGATTGTGGAACGTACAGCAGAAGTATTTGAAGTTGAAATTGATTCGTTAGCTGCACTAGAAATTG
CAAGACGTGCTCGTGGTACACCTCGTATTGCGAATCGTTTATTACGACGTGTACGAGATTTCGCACAAGTTCGTGGTAAC
GGAACAGTTACGATGGAAATTACGCAAATGGCATTAGAATTGCTGCAAGTAGATAAATTAGGTCTAGATCATATTGACCA
TAAATTATTGCTTGGTATTATTGAGAAATTCCGCGGTGGCCCAGTTGGACTAGAAACGGTTTCGGCAACGATTGGAGAAG
AATCTCATACGATTGAAGATGTGTATGAGCCATATTTATTACAAATTGGCTTTTTACAACGAACGCCAAGGGGCCGGATT
GTAACCCCGCTTGCATATGAGCATTTCGGAATGGAGATGCCAAAAGTATGA

Upstream 100 bases:

>100_bases
AACGAGAAGTTTCTCGCGTTGTACCAGAGTTATTAAAAGAATCATTAACGACGGATCAGTATATTAAAAAGGCACTTAGT
CTTTTACTAAATGGTAAGAG

Downstream 100 bases:

>100_bases
CAGAGATGCCGAAATTGCTTATTACAGCCGGTATCTTACTTATCGTTGTTGGATTAGCTTGGAAGTTCATTGGAAGGCTT
CCAGGCGATATTTTTGTGAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQDKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEM
GVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVG
ATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGN
GTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIEKFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRI
VTPLAYEHFGMEMPKV

Sequences:

>Translated_336_residues
MSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQDKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEM
GVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVG
ATTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGN
GTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIEKFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRI
VTPLAYEHFGMEMPKV
>Mature_335_residues
SIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQDKAKHNLEVFIEAAKMREETLDHVLLYGPPGLGKTTLANIIANEMG
VNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGA
TTRAGALSAPLRDRFGVLSRLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGNG
TVTMEITQMALELLQVDKLGLDHIDHKLLLGIIEKFRGGPVGLETVSATIGEESHTIEDVYEPYLLQIGFLQRTPRGRIV
TPLAYEHFGMEMPKV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=331, Percent_Identity=60.7250755287009, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787119, Length=128, Percent_Identity=35.9375, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37387; Mature: 37255

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQDKAKHNLEVFIEAAKMREETLDHVL
CCCHHHHHHCCCCCCCCCCCCEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEE
LYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDEIHRLH
EECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHH
RSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRAGALSAPLRDRFGVLS
HHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHH
RLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
GTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIEKFRGGPVGLETVSATIGEESHTIED
CEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHH
VYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPKV
HHHHHHHHHHHHHCCCCCCEECCHHHHHHCCCCCCC
>Mature Secondary Structure 
SIMDERLLSGESAYEDADLEYSLRPQTLRQYIGQDKAKHNLEVFIEAAKMREETLDHVL
CCHHHHHHCCCCCCCCCCCCEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEE
LYGPPGLGKTTLANIIANEMGVNVRTTSGPAIERPGDLAAVLTSLQPGDVLFIDEIHRLH
EECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHH
RSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRAGALSAPLRDRFGVLS
HHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHH
RLEYYTVDQLSAIVERTAEVFEVEIDSLAALEIARRARGTPRIANRLLRRVRDFAQVRGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
GTVTMEITQMALELLQVDKLGLDHIDHKLLLGIIEKFRGGPVGLETVSATIGEESHTIED
CEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHH
VYEPYLLQIGFLQRTPRGRIVTPLAYEHFGMEMPKV
HHHHHHHHHHHHHCCCCCCEECCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA