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The map label for this gene is megL

Identifier: 49481408

GI number: 49481408

Start: 4451425

End: 4452603

Strand: Reverse

Name: megL

Synonym: BT9727_4388

Alternate gene names: 49481408

Gene position: 4452603-4451425 (Counterclockwise)

Preceding gene: 49481693

Following gene: 49478718

Centisome position: 85.01

GC content: 39.19

Gene sequence:

>1179_bases
ATGAAAAAGAAGCATATGGAGACAGCGTTAATTCATCACGGTTATACATCAGAGGAACATAAAGGAAGTTTAACACCGCC
TTTATTTCAAACGTCTACATTTACATTTGAGACTGCGCAGCAAGGAGAGGCGAGTTTTGCGGGAGTGGATCCATCTTATA
TTTACTCAAGACTTGGAAATCCGACTGTGAAATTATTTGAAGAACGTATGGCGGTGTTAGAAGGTGGAGAAGAAGCACTT
GCTTTCGGATCCGGAATGGCAGCTATTTCAGCTACTTTAATTGGTTTTCTAAAAGCTGGAGATCATATTATTTGTTCAAA
TGGATTATACGGATGCACGTACGGCTTTTTAGAAGTGTTAGAAGAAAAATTTATGATTACGCATTCGTTTTGTGATATGG
AGACAGAGGGTGATATTGAAAATAAAATTTGCCCAAATACAAAGCTTATTTTCGTTGAAACACCAATTAATCCGACAATG
AAACTAATTGATTTAAAACAAGTGATTCGGGTTGCGAAGCGAAATGGGTTACTTGTCATTGTTGATAATACGTTTTGTTC
ACCTTATTTACAAAGACCTCTTGAACTTGGCTGTGACGCGGTTGTTCATAGTGCGACAAAATATATTGGTGGCCACGGTG
ATGTTGTGGCGGGTGTAACAATTTGTAAAACGAGAGCGTTAGCTGAAAAAATCCGCCCGATGCGAAAAGATATCGGCGGT
ATTATGGCACCGTTTGATGCATGGTTATTGTTACGCGGATTAAAGACACTAGCGGTAAGAATGGACCGCCATTGTGATAA
TGCAGAAAAAATTGTATCGTTCCTAAAAAATCATGATGCGGTAGAAGGTGTTTGGTATCCAGAAGGGGAGTTAGCATCTC
GCCAAATGAAACGGGGCGGCGGCGTTATATCTTTTTCAATTAAAGGCGGGAAAGAAGAGACGCAAGCGTTTATTAATGAC
CTCTACTTTATTACAATTGCAGTCAGTTTGGGAGATACAGAAACGTTAATTCAGCACCCGGCAACGATGACACACGCTGC
GATTCCAGCTGAGTTAAGACAAGAAATGGGTATTTATGATAATTTAATACGTTTATCTGTCGGTTTAGAATCGTGGGAGG
ATATCGTTTCTGATTTAGAGCAAGCATTAAAGAAAATATCTACTGTTAGTAATCAATAA

Upstream 100 bases:

>100_bases
TAGAAACACAAATAAAACGCTTACATAAAATGAAGTAAAACGTTCATCAAATTTTTAATGATGAATATGCGGCAATAAAA
ACATAAAAGGGGATGTTTTT

Downstream 100 bases:

>100_bases
AAAAAGAGCTAGCTATCGGTTTTGTACGTAATGTACTCAATCGATAACTAGCTCTTTTTTGTTACATATGTTTTAGGAGT
GTTTCCACAAACTTTTCTAA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKICPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Sequences:

>Translated_392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKICPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
>Mature_392_residues
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKICPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=40.6169665809769, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI299473759, Length=389, Percent_Identity=36.2467866323907, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=36.0824742268041, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=36.4102564102564, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=27.455919395466, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=40.2564102564103, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=41.2820512820513, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=39.1891891891892, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI193202265, Length=88, Percent_Identity=37.5, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=39.1304347826087, Blast_Score=265, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6323333, Length=446, Percent_Identity=30.2690582959641, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=416, Percent_Identity=30.2884615384615, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=30.25, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=320, Percent_Identity=30.3125, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922554, Length=385, Percent_Identity=38.1818181818182, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43211; Mature: 43211

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEERMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEGDIENKICPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCC
GVISFSIKGGKEETQAFINDLYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYD
CEEEEEECCCHHHHHHHHHHEEEEEEEEECCCHHHHHHCCCHHHHHHCCHHHHHHCCHHH
NLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PTVKLFEERMAVLEGGEEALAFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVL
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
EEKFMITHSFCDMETEGDIENKICPNTKLIFVETPINPTMKLIDLKQVIRVAKRNGLLVI
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
VDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
ECCCCCCHHHHCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCC
GVISFSIKGGKEETQAFINDLYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYD
CEEEEEECCCHHHHHHHHHHEEEEEEEEECCCHHHHHHCCCHHHHHHCCHHHHHHCCHHH
NLIRLSVGLESWEDIVSDLEQALKKISTVSNQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]