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The map label for this gene is comEC

Identifier: 49481367

GI number: 49481367

Start: 4126397

End: 4128718

Strand: Reverse

Name: comEC

Synonym: BT9727_4061

Alternate gene names: 49481367

Gene position: 4128718-4126397 (Counterclockwise)

Preceding gene: 49481368

Following gene: 49478609

Centisome position: 78.83

GC content: 36.22

Gene sequence:

>2322_bases
TTGCAAGGACAATGGGGCTACGTTGCAATCTCATTTATAATAGGGATTGCAATCGCCTTCTCCTCTTCAGTTGTATTGCT
GACTTGTTGTCTCGGTCTATATGTTTTCTTTTGTTTGTATCGTACTTCGCGTAAAACCTTCCTATATTGTATGATAGTGT
GTTTTAGTGGCGCTATGTACACCACGTATGTTCAAGGACAAAATAAGCCTCTGGGAGAGTCCTACGAAGCTACAAGAGGA
GTGATTTATAATACACCTCTTATTAACGGGGATCGCCTATCATTTCAAGTTGAAGATCAGAATAAAAATATAGTGCAGTT
AAGTTACAAAATGAAATCAGCCTCGGAAAAGAAACAAATGCGACAATTACATGCAGGAGTGTCATGTATATTTGATGGTG
AGAGGAAAGAACCACAAATAGCTCGGAATTTTCATGGGTTTAATTATCGTGATTATTTATATAAGCAAAATATTCATTTC
ATATTGGAAGCTACATATATTTCTGAATGCCGTAAAACATCGTTGTCACTTGTGCAATGGATTCTTCTTTTGAGGCAGCA
AGCAATCTTAGGAGTTACAGAAATGTTTCCAGAGCAATCAGGCGCTTTTATGAACGCGTTACTATTTGGTGATAGACAAC
AAATGACATTTGAAGTTGAAGGGCAATATCAACAATTCGGTCTTGTGCATTTGTTGGCGATTTCAGGATCGCATATCGTA
TTGTTAATGGTGATTGTGTATTTTATTTTGCTAAGAAGTGGTGTGACAAGGGAGATAGCAACAGTATGTCTTATCTTCTT
CATTCCTATATATATGATTTTAGCAGGAGCGTCACCGTCTGTTATAAGAGCTTCTATAACAGGAGTTTTAATGTTAATTG
CTTTTATGTGTTCTATTCGTTTATCTAGCTTAGATGCTTTAAGTATAACAGCTATATGTATGCTTATATTTGATCCATAT
CTCGTGTTTAATATTGGCTTTCAGTTTTCTTTCGTTGGGAGTTTTGCTTTACTTTTATCTGCCCCGCTCTTACTAGAGAG
TGGTAATGGAGTAATTAGAAATTCTATTTATATTTCTCTTATTTCACAGCTCGTTAGTACTCCGATTTTGTTATATCACT
TCGGTTATTATTCTCCATATAGTATTTTTCTAAATATCCTTTACGTTCCGTTTTTATCTCTCATTGTATTGCCGTGTAGT
ATTATTATTTTGATATGTTTGCCGATCATCCCGTTTCTTGCAAAAAGCTTTGCGAATGTACTATCAATAGGTTTGAATCT
TTCTAATGATTTTTTAAGTTATTGTGAAAGTTTACCATTTACCCGTCTTAATTTCGGGCAAACACCTATACTTCTTGTAG
CCTTATATTGCGTAAGTATTATTAGTGTATTGATGGTTTGGGAAAGGCGAATATCTAAGGGAATGGTGTTTATATTTGCG
GGCATATTTCTTTTTATTAGTACAGGTCATTATGTATATCCGTATTTTCGAGAAAGTGGGAGTGTTACATTTCTTGATGT
TGGCCAGGGGGATGCAATATTAATTCGCCTCCCGTATGATCAAGAGATTTACCTTATTGACACTGGTGGAACAATTCGTT
TAAACAAGGAAGAATGGCAACGGAAAAAACATGAATTTTCTGTTGGAAATGATATTTTGATCCCTTATTTACAAAAGGAA
GGTATTAAAAAAATTGATAAATTAATTGTAACGCATGGAGATGCAGATCATATCGGTGCTGCACAAGAATTATTATCAAA
TATAACCGTAAAAGAAGTTGTATTTGGTCGAAAGGAACAAGAGGCAATATTAGAAAAAGCAGTAAAGAAACAGGCGTTAG
AAAAGGAAGTGAAAATAAGTGAAGTGGGGGAAGGAGAGAGTTGGCGCGTAAATGAAGCGGAATTTTTTGTGCTAGCACCA
ACAGGGAAAGAAAGAAGTGAAAATAACGCTTCAATTGTACTGTGGGCAAAATTAGGAGGGATAACGTGGCTGTTTACAGG
TGATTTAGAAGAAGAAGGAGAGAAGGGTTTAGTAGCTACATATCCAGATTTACGGGCGGATGTTTTAAAGGTTGCTCATC
ATGGAAGTAATACGTCATCTATAACGCCTTTTTTGAGCGCCGTACAGCCTAATATAGCGATTATTTCTGTCGGTGAACGG
AATAGGTATGGGCACCCTCATAAGGAAGTTATAGAGCGTTTTGAGAAGATGGCGATTGAAATATGGCGCACGGATAAGCA
AGGTGCTATTTCCTATGTTTTTAAAGAGGAACGCGGAACGTTTCGTAGCAAAATCACATATGATGAAACACGGAATAGAT
AA

Upstream 100 bases:

>100_bases
AAGAACTATTTGCATCTTTAGAAAAAGAGAACTTATCAATGGAAGAATTACAGCATGTATTCACGAAAGCAAAAATGATG
CTATAAGGAGTGAGTATGAG

Downstream 100 bases:

>100_bases
AAAAAAGACTGCTAGTAAGCAGCAGTCTTTAAGAACCGATAAATTTAATAACAGTTGCAATGATGAAAAGTGTTGCGAAA
AAACCAAATGAGACGATAAA

Product: DNA internalization-related competence protein ComEC/Rec2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MQGQWGYVAISFIIGIAIAFSSSVVLLTCCLGLYVFFCLYRTSRKTFLYCMIVCFSGAMYTTYVQGQNKPLGESYEATRG
VIYNTPLINGDRLSFQVEDQNKNIVQLSYKMKSASEKKQMRQLHAGVSCIFDGERKEPQIARNFHGFNYRDYLYKQNIHF
ILEATYISECRKTSLSLVQWILLLRQQAILGVTEMFPEQSGAFMNALLFGDRQQMTFEVEGQYQQFGLVHLLAISGSHIV
LLMVIVYFILLRSGVTREIATVCLIFFIPIYMILAGASPSVIRASITGVLMLIAFMCSIRLSSLDALSITAICMLIFDPY
LVFNIGFQFSFVGSFALLLSAPLLLESGNGVIRNSIYISLISQLVSTPILLYHFGYYSPYSIFLNILYVPFLSLIVLPCS
IIILICLPIIPFLAKSFANVLSIGLNLSNDFLSYCESLPFTRLNFGQTPILLVALYCVSIISVLMVWERRISKGMVFIFA
GIFLFISTGHYVYPYFRESGSVTFLDVGQGDAILIRLPYDQEIYLIDTGGTIRLNKEEWQRKKHEFSVGNDILIPYLQKE
GIKKIDKLIVTHGDADHIGAAQELLSNITVKEVVFGRKEQEAILEKAVKKQALEKEVKISEVGEGESWRVNEAEFFVLAP
TGKERSENNASIVLWAKLGGITWLFTGDLEEEGEKGLVATYPDLRADVLKVAHHGSNTSSITPFLSAVQPNIAIISVGER
NRYGHPHKEVIERFEKMAIEIWRTDKQGAISYVFKEERGTFRSKITYDETRNR

Sequences:

>Translated_773_residues
MQGQWGYVAISFIIGIAIAFSSSVVLLTCCLGLYVFFCLYRTSRKTFLYCMIVCFSGAMYTTYVQGQNKPLGESYEATRG
VIYNTPLINGDRLSFQVEDQNKNIVQLSYKMKSASEKKQMRQLHAGVSCIFDGERKEPQIARNFHGFNYRDYLYKQNIHF
ILEATYISECRKTSLSLVQWILLLRQQAILGVTEMFPEQSGAFMNALLFGDRQQMTFEVEGQYQQFGLVHLLAISGSHIV
LLMVIVYFILLRSGVTREIATVCLIFFIPIYMILAGASPSVIRASITGVLMLIAFMCSIRLSSLDALSITAICMLIFDPY
LVFNIGFQFSFVGSFALLLSAPLLLESGNGVIRNSIYISLISQLVSTPILLYHFGYYSPYSIFLNILYVPFLSLIVLPCS
IIILICLPIIPFLAKSFANVLSIGLNLSNDFLSYCESLPFTRLNFGQTPILLVALYCVSIISVLMVWERRISKGMVFIFA
GIFLFISTGHYVYPYFRESGSVTFLDVGQGDAILIRLPYDQEIYLIDTGGTIRLNKEEWQRKKHEFSVGNDILIPYLQKE
GIKKIDKLIVTHGDADHIGAAQELLSNITVKEVVFGRKEQEAILEKAVKKQALEKEVKISEVGEGESWRVNEAEFFVLAP
TGKERSENNASIVLWAKLGGITWLFTGDLEEEGEKGLVATYPDLRADVLKVAHHGSNTSSITPFLSAVQPNIAIISVGER
NRYGHPHKEVIERFEKMAIEIWRTDKQGAISYVFKEERGTFRSKITYDETRNR
>Mature_773_residues
MQGQWGYVAISFIIGIAIAFSSSVVLLTCCLGLYVFFCLYRTSRKTFLYCMIVCFSGAMYTTYVQGQNKPLGESYEATRG
VIYNTPLINGDRLSFQVEDQNKNIVQLSYKMKSASEKKQMRQLHAGVSCIFDGERKEPQIARNFHGFNYRDYLYKQNIHF
ILEATYISECRKTSLSLVQWILLLRQQAILGVTEMFPEQSGAFMNALLFGDRQQMTFEVEGQYQQFGLVHLLAISGSHIV
LLMVIVYFILLRSGVTREIATVCLIFFIPIYMILAGASPSVIRASITGVLMLIAFMCSIRLSSLDALSITAICMLIFDPY
LVFNIGFQFSFVGSFALLLSAPLLLESGNGVIRNSIYISLISQLVSTPILLYHFGYYSPYSIFLNILYVPFLSLIVLPCS
IIILICLPIIPFLAKSFANVLSIGLNLSNDFLSYCESLPFTRLNFGQTPILLVALYCVSIISVLMVWERRISKGMVFIFA
GIFLFISTGHYVYPYFRESGSVTFLDVGQGDAILIRLPYDQEIYLIDTGGTIRLNKEEWQRKKHEFSVGNDILIPYLQKE
GIKKIDKLIVTHGDADHIGAAQELLSNITVKEVVFGRKEQEAILEKAVKKQALEKEVKISEVGEGESWRVNEAEFFVLAP
TGKERSENNASIVLWAKLGGITWLFTGDLEEEGEKGLVATYPDLRADVLKVAHHGSNTSSITPFLSAVQPNIAIISVGER
NRYGHPHKEVIERFEKMAIEIWRTDKQGAISYVFKEERGTFRSKITYDETRNR

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=601, Percent_Identity=22.2961730449251, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 87269; Mature: 87269

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGQWGYVAISFIIGIAIAFSSSVVLLTCCLGLYVFFCLYRTSRKTFLYCMIVCFSGAMY
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEE
TTYVQGQNKPLGESYEATRGVIYNTPLINGDRLSFQVEDQNKNIVQLSYKMKSASEKKQM
EEEEECCCCCCCCCHHHCCCEEEECCEECCCEEEEEEECCCCCEEEEEEHHHHHHHHHHH
RQLHAGVSCIFDGERKEPQIARNFHGFNYRDYLYKQNIHFILEATYISECRKTSLSLVQW
HHHHCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHH
ILLLRQQAILGVTEMFPEQSGAFMNALLFGDRQQMTFEVEGQYQQFGLVHLLAISGSHIV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHCCEEEEEECCCHHH
LLMVIVYFILLRSGVTREIATVCLIFFIPIYMILAGASPSVIRASITGVLMLIAFMCSIR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LSSLDALSITAICMLIFDPYLVFNIGFQFSFVGSFALLLSAPLLLESGNGVIRNSIYISL
HHCCCHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHCCCCEEECCCCEEHHHHHHHH
ISQLVSTPILLYHFGYYSPYSIFLNILYVPFLSLIVLPCSIIILICLPIIPFLAKSFANV
HHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIGLNLSNDFLSYCESLPFTRLNFGQTPILLVALYCVSIISVLMVWERRISKGMVFIFA
HHHCCCCCHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GIFLFISTGHYVYPYFRESGSVTFLDVGQGDAILIRLPYDQEIYLIDTGGTIRLNKEEWQ
HHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEECCCCEEEEEECCCEEEECHHHHH
RKKHEFSVGNDILIPYLQKEGIKKIDKLIVTHGDADHIGAAQELLSNITVKEVVFGRKEQ
HHHHCCCCCCCEEEHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHCCCHH
EAILEKAVKKQALEKEVKISEVGEGESWRVNEAEFFVLAPTGKERSENNASIVLWAKLGG
HHHHHHHHHHHHHHHHCCHHCCCCCCCEEECCCEEEEECCCCCCCCCCCCEEEEEEEECC
ITWLFTGDLEEEGEKGLVATYPDLRADVLKVAHHGSNTSSITPFLSAVQPNIAIISVGER
EEEEEECCCHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCC
NRYGHPHKEVIERFEKMAIEIWRTDKQGAISYVFKEERGTFRSKITYDETRNR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHCEECHHCCCC
>Mature Secondary Structure
MQGQWGYVAISFIIGIAIAFSSSVVLLTCCLGLYVFFCLYRTSRKTFLYCMIVCFSGAMY
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEE
TTYVQGQNKPLGESYEATRGVIYNTPLINGDRLSFQVEDQNKNIVQLSYKMKSASEKKQM
EEEEECCCCCCCCCHHHCCCEEEECCEECCCEEEEEEECCCCCEEEEEEHHHHHHHHHHH
RQLHAGVSCIFDGERKEPQIARNFHGFNYRDYLYKQNIHFILEATYISECRKTSLSLVQW
HHHHCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHH
ILLLRQQAILGVTEMFPEQSGAFMNALLFGDRQQMTFEVEGQYQQFGLVHLLAISGSHIV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHCCEEEEEECCCHHH
LLMVIVYFILLRSGVTREIATVCLIFFIPIYMILAGASPSVIRASITGVLMLIAFMCSIR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LSSLDALSITAICMLIFDPYLVFNIGFQFSFVGSFALLLSAPLLLESGNGVIRNSIYISL
HHCCCHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHCCCCEEECCCCEEHHHHHHHH
ISQLVSTPILLYHFGYYSPYSIFLNILYVPFLSLIVLPCSIIILICLPIIPFLAKSFANV
HHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIGLNLSNDFLSYCESLPFTRLNFGQTPILLVALYCVSIISVLMVWERRISKGMVFIFA
HHHCCCCCHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GIFLFISTGHYVYPYFRESGSVTFLDVGQGDAILIRLPYDQEIYLIDTGGTIRLNKEEWQ
HHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEECCCCEEEEEECCCEEEECHHHHH
RKKHEFSVGNDILIPYLQKEGIKKIDKLIVTHGDADHIGAAQELLSNITVKEVVFGRKEQ
HHHHCCCCCCCEEEHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHCCCHH
EAILEKAVKKQALEKEVKISEVGEGESWRVNEAEFFVLAPTGKERSENNASIVLWAKLGG
HHHHHHHHHHHHHHHHCCHHCCCCCCCEEECCCEEEEECCCCCCCCCCCCEEEEEEEECC
ITWLFTGDLEEEGEKGLVATYPDLRADVLKVAHHGSNTSSITPFLSAVQPNIAIISVGER
EEEEEECCCHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCC
NRYGHPHKEVIERFEKMAIEIWRTDKQGAISYVFKEERGTFRSKITYDETRNR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHCEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]