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The map label for this gene is yhfE

Identifier: 49481331

GI number: 49481331

Start: 4368752

End: 4369837

Strand: Reverse

Name: yhfE

Synonym: BT9727_4302

Alternate gene names: 49481331

Gene position: 4369837-4368752 (Counterclockwise)

Preceding gene: 49480685

Following gene: 49480684

Centisome position: 83.43

GC content: 40.33

Gene sequence:

>1086_bases
ATGACAAAATTAGACGAGACATTGACAATGCTAAAAGAATTAACAGATGCACGCGGTATTGCGGGTAACGAGCGTGAACC
ACGTGAAGTAATGAAGAAATATATTGAGCCGTTTGCCGACGAACTTTCTACTGATAATTTAGGAAGTTTAGTTGCGAAAA
AAGTAGGGGAAGAAAACGGCCCGAAAATTATGGTTGCAGGTCATTTAGATGAAGTTGGCTTTATGATTACGCAAATTGAT
GACAAAGGTTTCCTTCGTTTCCAAACAGTTGGTGGCTGGTGGTCACAAGTTATGCTTGCGCAGCGCGTGACAATTGTAAC
GCGTAAAGGAGATGTAACAGGTGTAATCGGTTCAAAACCACCGCATATCTTACCTCCAGAAGCGCGTAAAAAACCAGTTG
AAATTAAAGACATGTTCATCGATATTGGTGCTTCTAGCCAAGAAGAAGCAATGGAGTGGGGCGTACGACCAGGAGATCAA
GTTGTACCTTACTTTGAATTCCAAGTGATGAAGAATGAAAAAATGTTACTTGCAAAAGCATGGGATAACCGAATTGGTTG
TGCAATTGCAATTGACGTATTAAAACAATTAAAAGATGAAAAGCATCCAAACGTTGTATACGGCGTAGGGACTGTACAAG
AAGAAGTCGGTCTTCGTGGTGCGAAAACATCTGCAAATTATATTAAACCAGATATCGCATTCGCAGTAGATGTTGGTATC
GCTGGTGACACACCAGGGGTAACGTCAAAAGAAGCGCAAAGTAAAATGGGTGATGGCCCACAGATCATTTTATATGATGC
TTCTGTTATCGGTCATACCGGTTTACGTGACTTTGTAGTTGATGTTGCTGATGAATTACAAATTCCGTATCAATATGATT
CAGTAGCGGGCGGCGGAACGGATGCAGGTGCAATTCATATTGCTGTAAATGGTATTCCGTCTATGGCAATTACGATCGCA
ACGCGTTATATTCATTCTCATGCGGCAATGTTACACCGTGATGACTATGAAAATGCAGTGAAGTTAATTGTAGAAGTTAT
TAAACGTCTTGATAAAGAGGCTGTACATAACATTACATTTAATTAA

Upstream 100 bases:

>100_bases
AAATCATCAGCGTCAAACACAAGGATACTAATTTAATTTTTGAATATTTCAATTTCCTTTTGTATAATGAAGTGACTGAA
GAAAAAGGAGGGTGTTGGCA

Downstream 100 bases:

>100_bases
TAGAAAAAGCGAAGGAGCCTCTCCTTCGCTTTTTTATGTTTTAAAAAGGTCGTTCTAATCCCGCTGCAGTATGAGCCCCT
AAAATGATTAAACGAGTTAA

Product: glucanase/ deblocking aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTKLDETLTMLKELTDARGIAGNEREPREVMKKYIEPFADELSTDNLGSLVAKKVGEENGPKIMVAGHLDEVGFMITQID
DKGFLRFQTVGGWWSQVMLAQRVTIVTRKGDVTGVIGSKPPHILPPEARKKPVEIKDMFIDIGASSQEEAMEWGVRPGDQ
VVPYFEFQVMKNEKMLLAKAWDNRIGCAIAIDVLKQLKDEKHPNVVYGVGTVQEEVGLRGAKTSANYIKPDIAFAVDVGI
AGDTPGVTSKEAQSKMGDGPQIILYDASVIGHTGLRDFVVDVADELQIPYQYDSVAGGGTDAGAIHIAVNGIPSMAITIA
TRYIHSHAAMLHRDDYENAVKLIVEVIKRLDKEAVHNITFN

Sequences:

>Translated_361_residues
MTKLDETLTMLKELTDARGIAGNEREPREVMKKYIEPFADELSTDNLGSLVAKKVGEENGPKIMVAGHLDEVGFMITQID
DKGFLRFQTVGGWWSQVMLAQRVTIVTRKGDVTGVIGSKPPHILPPEARKKPVEIKDMFIDIGASSQEEAMEWGVRPGDQ
VVPYFEFQVMKNEKMLLAKAWDNRIGCAIAIDVLKQLKDEKHPNVVYGVGTVQEEVGLRGAKTSANYIKPDIAFAVDVGI
AGDTPGVTSKEAQSKMGDGPQIILYDASVIGHTGLRDFVVDVADELQIPYQYDSVAGGGTDAGAIHIAVNGIPSMAITIA
TRYIHSHAAMLHRDDYENAVKLIVEVIKRLDKEAVHNITFN
>Mature_360_residues
TKLDETLTMLKELTDARGIAGNEREPREVMKKYIEPFADELSTDNLGSLVAKKVGEENGPKIMVAGHLDEVGFMITQIDD
KGFLRFQTVGGWWSQVMLAQRVTIVTRKGDVTGVIGSKPPHILPPEARKKPVEIKDMFIDIGASSQEEAMEWGVRPGDQV
VPYFEFQVMKNEKMLLAKAWDNRIGCAIAIDVLKQLKDEKHPNVVYGVGTVQEEVGLRGAKTSANYIKPDIAFAVDVGIA
GDTPGVTSKEAQSKMGDGPQIILYDASVIGHTGLRDFVVDVADELQIPYQYDSVAGGGTDAGAIHIAVNGIPSMAITIAT
RYIHSHAAMLHRDDYENAVKLIVEVIKRLDKEAVHNITFN

Specific function: Putative aminopeptidase [H]

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=355, Percent_Identity=40.5633802816901, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788727, Length=352, Percent_Identity=33.2386363636364, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87082417, Length=343, Percent_Identity=30.9037900874636, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 39569; Mature: 39438

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLDETLTMLKELTDARGIAGNEREPREVMKKYIEPFADELSTDNLGSLVAKKVGEENG
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
PKIMVAGHLDEVGFMITQIDDKGFLRFQTVGGWWSQVMLAQRVTIVTRKGDVTGVIGSKP
CEEEEEECCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHEEEEEECCCEEEECCCCC
PHILPPEARKKPVEIKDMFIDIGASSQEEAMEWGVRPGDQVVPYFEFQVMKNEKMLLAKA
CCCCCCHHCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEC
WDNRIGCAIAIDVLKQLKDEKHPNVVYGVGTVQEEVGLRGAKTSANYIKPDIAFAVDVGI
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEEECC
AGDTPGVTSKEAQSKMGDGPQIILYDASVIGHTGLRDFVVDVADELQIPYQYDSVAGGGT
CCCCCCCCCHHHHHHCCCCCEEEEEECHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCCC
DAGAIHIAVNGIPSMAITIATRYIHSHAAMLHRDDYENAVKLIVEVIKRLDKEAVHNITF
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
N
C
>Mature Secondary Structure 
TKLDETLTMLKELTDARGIAGNEREPREVMKKYIEPFADELSTDNLGSLVAKKVGEENG
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
PKIMVAGHLDEVGFMITQIDDKGFLRFQTVGGWWSQVMLAQRVTIVTRKGDVTGVIGSKP
CEEEEEECCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHEEEEEECCCEEEECCCCC
PHILPPEARKKPVEIKDMFIDIGASSQEEAMEWGVRPGDQVVPYFEFQVMKNEKMLLAKA
CCCCCCHHCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEC
WDNRIGCAIAIDVLKQLKDEKHPNVVYGVGTVQEEVGLRGAKTSANYIKPDIAFAVDVGI
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEEECC
AGDTPGVTSKEAQSKMGDGPQIILYDASVIGHTGLRDFVVDVADELQIPYQYDSVAGGGT
CCCCCCCCCHHHHHHCCCCCEEEEEECHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCCC
DAGAIHIAVNGIPSMAITIATRYIHSHAAMLHRDDYENAVKLIVEVIKRLDKEAVHNITF
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]