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The map label for this gene is vacB

Identifier: 49481126

GI number: 49481126

Start: 4857959

End: 4860373

Strand: Reverse

Name: vacB

Synonym: BT9727_4808

Alternate gene names: 49481126

Gene position: 4860373-4857959 (Counterclockwise)

Preceding gene: 49481488

Following gene: 49478891

Centisome position: 92.8

GC content: 41.12

Gene sequence:

>2415_bases
TTGGAAGAAATCATACAAGAACATATTGATAAGTTGTTATTATTTATGAGAGAAGAAGCGTATAAACCGCTAACGATACA
AGAGTTAGAAGAGGCATTTGGGATTGAAGGTTCTGAGGGCTTCAAAGATTTCGTAAAGGCACTTGTAACGATGGAAGAAA
AGGGACTTGTTATTCGTACTCGTAGCAACCGTTACGGTCTTCCTGAAAAGATGAATTTAATACGTGGTAAGTTAATTGGA
CATGCACGTGGTTTTGCATTTGTTGTACCAGACGAGAAGAAAACGGGAGACGATGATCTTTTCATCCCGCCTACAGAATT
AAACGGTGCGCTTCATGGTGATACAGTATTAGCACGCCTTAGTTCCCAATCGAGTGGTTCGCGTCAAGAAGGTTCTATTG
TACGCATTTTAGAACGTGGCACGAAAGAATTAGTTGGTACATATACAGAATCGAAAAACTTTGGATTTGTTATACCTGAC
AATAAACGTTGGACAAGTGACATTTTCGTATTGAAAAGCGCATCAATGGGCGCTGTAGAAGGTCATAAAGTAGTTGTGAA
AATTACGAGCTATCCAGAGAATCGTTTAAGTGCAGAAGGTGAAGTTATTCAAATTCTAGGTCATAAAAATGATCCAGGAG
TAGATATTTTATCTGTCATTCATAAACATCACTTGCCTTTAGCGTTCCCTGAAGAAGTGATGGAACATGCAAACAGTGTA
CCAGAAACGATTTCAGAAGAAGATTTAAAAGATCGCCGTGACCTGCGTGACCAAATGATCGTAACAATTGACGGTGCAGA
CGCAAAAGATTTAGATGACGCTGTTACAGTAACGAAGCTCGAGAACGGTAACTATAAACTTGGCGTTCACATTGCGGATG
TAAGTCATTACGTTCAAGAAGGTTCTCCAATTGATGTAGAAGCAGCGGAAAGAGCGACGAGTGTGTATCTTGTTGACCGT
GTAATTCCGATGATTCCACATCGTCTATCTAACGGTATTTGTTCATTAAATCCGAAAGTAGACCGTCTGACGTTATCTTG
TGAAATGGAAATCAACAACTTAGGTGACGTTGTCAAACACGAGATTTTCCAAAGTGTAATTAAAACGACAGAGCGTATGA
CGTATGCTGACGTAAGAAGCATTTTAGAAGATGAGGACGAAGAGCTAATTAAGCGTTATGAACCGTTAGTGCCGATGTTT
AAAGAGATGGGGCAATTAGCACAAATTTTACGTGAAAAACGTATGCGCCGCGGTGCAATCGACTTTGACTTTAAAGAAGC
GAAAGTATTAGTGGATGAAGAAGGAAAACCGACAGATGTTGTCATGCGTGATCGTTCTGTATCAGAGAAGTTAATTGAAG
AATTTATGCTTGTTGCGAACGAAACAGTAGCAGAGCACTTCCACTGGATGAACGTACCATTCATGTATCGTGTCCATGAA
GATCCGAAAGAAGATAAGTTAGAGCGTTTCTTCGAGTTTGTAACGAACTTCGGATATGCGGTAAAAGGACGTGCGAATGA
AGTGCATCCTCGTGCGCTGCAACAAATTCTTGAAATGGTTCAAGGACAGCCTGAAGAAGTAGTCATTTCAACAGTTATGC
TTCGTTCTATGAAGCAAGCACGTTACGATGCAGATAGCTTAGGACACTTTGGTTTATCAACTGAATTCTACACACATTTC
ACATCGCCAATTCGTCGTTACCCAGATACGATTGTTCATAGATTAATTCGTGAATACATCATTAACGGTAAAGTCGACAA
TGAAACACAAGCAAAGTGGCGTGAAAAATTACCTGAGATTGCAGAACACTCTTCTAATATGGAGCGTCGTGCTGTTGAAG
CAGAACGTGAAACAGATGAGCTGAAAAAAGCAGAATACATGCTTGATAAGATTGGGGAAGAGTATGACGGTATGATTAGC
TCTGTAACAAACTTCGGTTTATTCGTAGAGCTTCCAAATACAATTGAAGGTCTTGTACACGTTAGCTACTTAACGGATGA
TTACTACCGTTACGATGAGCAGCATTTCGCAATGATCGGAGAACGTACAGGTAACGTATTCCGCATCGGTGACGAAATTA
CAATTCGTGTTATTAATGTAAACAAAGACGAGCGTGCAATCGACTTTGAAATCGTTGGCATGAAAGGTACACCTCGTCGT
AAGTTCAAAGACCGCCCAGTCGTTATTGAACAGCCAAGAACAGGCAGAAAGAAACGCGGGGGACGCAGTGAGCGCAGTAA
TGAGCGTGGCGGAGAACGTGGCACAGGTAGAAAATTTGACCGTGGTGGCAAAGGGAAAGGAAGAGGATCCGCATCCGCTA
GCCAGCCAGGGAAAAAAGATGGTAACGGTAAGAAGAAAAAAGCATTCTTCGAAAACGTACCAGGATTCAAGAAGAAAAAG
AAAAAGCGTAAGTAA

Upstream 100 bases:

>100_bases
ACTGTAAAAATCATAAGGGGAGTATTGAAGTTTCGCTTTATACATCCCCTATCTTTCGGATACACTAAATAATATAAGGT
TTTAAAGGAGGTATTTTTGC

Downstream 100 bases:

>100_bases
GAAAAGGATGGCTTCGCTTTGATGAAAAGCGAGGCTTTTTCTTTTTTATTGGTGGCTTGAGAGCGAGCCACTTTCCTCTT
TTGTTGGAATCCAGCTACAG

Product: ribonuclease R (RNase R)

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLIRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASQPGKKDGNGKKKKAFFENVPGFKKKK
KKRK

Sequences:

>Translated_804_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLIRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASQPGKKDGNGKKKKAFFENVPGFKKKK
KKRK
>Mature_804_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLIRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASQPGKKDGNGKKKKAFFENVPGFKKKK
KKRK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=458, Percent_Identity=31.0043668122271, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI190014623, Length=462, Percent_Identity=30.952380952381, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI134288890, Length=414, Percent_Identity=34.0579710144928, Blast_Score=217, Evalue=3e-56,
Organism=Homo sapiens, GI19115966, Length=575, Percent_Identity=28.1739130434783, Blast_Score=192, Evalue=1e-48,
Organism=Homo sapiens, GI219521928, Length=575, Percent_Identity=28.1739130434783, Blast_Score=191, Evalue=3e-48,
Organism=Escherichia coli, GI87082383, Length=725, Percent_Identity=38.7586206896552, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1787542, Length=639, Percent_Identity=24.8826291079812, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553506, Length=473, Percent_Identity=31.0782241014799, Blast_Score=205, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI212645896, Length=492, Percent_Identity=28.8617886178862, Blast_Score=189, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6324552, Length=589, Percent_Identity=28.0135823429542, Blast_Score=206, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6320499, Length=422, Percent_Identity=25.5924170616114, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6323943, Length=432, Percent_Identity=23.1481481481481, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24649634, Length=475, Percent_Identity=31.1578947368421, Blast_Score=235, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24654592, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19922976, Length=363, Percent_Identity=32.5068870523416, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24654597, Length=363, Percent_Identity=32.5068870523416, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 91727; Mature: 91727

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLIRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDADSLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASQPGKKD
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GNGKKKKAFFENVPGFKKKKKKRK
CCCCHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLIRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDADSLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASQPGKKD
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GNGKKKKAFFENVPGFKKKKKKRK
CCCCHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]