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The map label for this gene is dapF

Identifier: 49481006

GI number: 49481006

Start: 4708257

End: 4709123

Strand: Reverse

Name: dapF

Synonym: BT9727_4647

Alternate gene names: 49481006

Gene position: 4709123-4708257 (Counterclockwise)

Preceding gene: 49480349

Following gene: 49481005

Centisome position: 89.91

GC content: 39.79

Gene sequence:

>867_bases
ATGAGCCAATTTTCTTTTACAAAAATGCATGGTCTTGGCAATAGTTATATATATGTAAATATGTTTGAAGAACAAATTCC
TGAAGAAGATTTAGCTCTTGTCGCTGAAAAGGTTTCAAATATTAATACTGGTATTGGAGCAGATGGAATGATTTTAATTT
GTCCATCTGACGTAGCACCTGTGAAAATGCGCATGTTTAATAATGATGGATCAGAAGGAAAGAGCTGCGGTAACGGTTTA
CGCTGCGTAGCGAAATATGCGTATGAGCATAAACTAGTAGAAGATACAGTCTTCACAATTGAAACGTTAGCTGGCATTGT
AACAGCGGAAGTAACGGTAGAAGAAGGGAAAGTTACGTTAGCGAAAATCGATATGGGAGCACCTCGTTTAACACGTGCAG
AAATACCGATGCTTGGTGAAGGTGAAACACCATTTATTCGTGAGAACTTCTTATACAATAATCATCGTTATGCATTTACA
GCTGTTTCAATGGGAAATCCGCATGCGGTTATTTTTGTGGATGATGTAGAACAAGCACCTCTTACAACACTTGGACCAGT
TCTTGAGACACATGAAATGTTCCCAGAACGAGTAAATGTTGAATTCATTGAGATTTTGAATGAGGAAGAGATGAACTTCC
GCGTTTGGGAACGTGGATCTGGTGTAACACAAGCTTGCGGAACAGGGGCGTGTGCTGCAGTTGTAGCCTCTATTTTAAAT
GGGAAAATGGAACGTGGTAAGGAAATTACAGTTCATTTAGCTGGCGGCGATTTAATGATTGCATGGACAGAAGAAGGCAA
CGTGTTAATGAAAGGACCAGCAGAAGTAATTTGCCACGGAGTGTATGAGTACAAGATAGAAGCGTAA

Upstream 100 bases:

>100_bases
CAAATGTTGTCAAATATGAGAAACGGGGGACATTCATATTGTGTTCTAAAAGCGTGCATTTTATACTTAACATAACAAAT
TATTTTTGGAGTGAATACAA

Downstream 100 bases:

>100_bases
AGATGTATAATAGAACGAGAACAGAAAGGATGGTGTATTTCATGATTGAAGTAACAGAACAAGCAGCTTTTCAAATTAAA
GATATGTTAAAAGATGCTGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSQFSFTKMHGLGNSYIYVNMFEEQIPEEDLALVAEKVSNINTGIGADGMILICPSDVAPVKMRMFNNDGSEGKSCGNGL
RCVAKYAYEHKLVEDTVFTIETLAGIVTAEVTVEEGKVTLAKIDMGAPRLTRAEIPMLGEGETPFIRENFLYNNHRYAFT
AVSMGNPHAVIFVDDVEQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILN
GKMERGKEITVHLAGGDLMIAWTEEGNVLMKGPAEVICHGVYEYKIEA

Sequences:

>Translated_288_residues
MSQFSFTKMHGLGNSYIYVNMFEEQIPEEDLALVAEKVSNINTGIGADGMILICPSDVAPVKMRMFNNDGSEGKSCGNGL
RCVAKYAYEHKLVEDTVFTIETLAGIVTAEVTVEEGKVTLAKIDMGAPRLTRAEIPMLGEGETPFIRENFLYNNHRYAFT
AVSMGNPHAVIFVDDVEQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILN
GKMERGKEITVHLAGGDLMIAWTEEGNVLMKGPAEVICHGVYEYKIEA
>Mature_287_residues
SQFSFTKMHGLGNSYIYVNMFEEQIPEEDLALVAEKVSNINTGIGADGMILICPSDVAPVKMRMFNNDGSEGKSCGNGLR
CVAKYAYEHKLVEDTVFTIETLAGIVTAEVTVEEGKVTLAKIDMGAPRLTRAEIPMLGEGETPFIRENFLYNNHRYAFTA
VSMGNPHAVIFVDDVEQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILNG
KMERGKEITVHLAGGDLMIAWTEEGNVLMKGPAEVICHGVYEYKIEA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=277, Percent_Identity=37.9061371841155, Blast_Score=177, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31595; Mature: 31463

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFSFTKMHGLGNSYIYVNMFEEQIPEEDLALVAEKVSNINTGIGADGMILICPSDVAP
CCCCCCCHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
VKMRMFNNDGSEGKSCGNGLRCVAKYAYEHKLVEDTVFTIETLAGIVTAEVTVEEGKVTL
EEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEE
AKIDMGAPRLTRAEIPMLGEGETPFIRENFLYNNHRYAFTAVSMGNPHAVIFVDDVEQAP
EEEECCCCCCCCCCCCCCCCCCCCEEECCEEECCCEEEEEEEECCCCEEEEEEECCCCCC
LTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILN
CHHHHHHHHHHHHCHHHCCCCEEEEECCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHC
GKMERGKEITVHLAGGDLMIAWTEEGNVLMKGPAEVICHGVYEYKIEA
CHHHCCCEEEEEEECCCEEEEEECCCCEEEECCHHHHEECEEEEEECC
>Mature Secondary Structure 
SQFSFTKMHGLGNSYIYVNMFEEQIPEEDLALVAEKVSNINTGIGADGMILICPSDVAP
CCCCCCHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
VKMRMFNNDGSEGKSCGNGLRCVAKYAYEHKLVEDTVFTIETLAGIVTAEVTVEEGKVTL
EEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEE
AKIDMGAPRLTRAEIPMLGEGETPFIRENFLYNNHRYAFTAVSMGNPHAVIFVDDVEQAP
EEEECCCCCCCCCCCCCCCCCCCCEEECCEEECCCEEEEEEEECCCCEEEEEEECCCCCC
LTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILN
CHHHHHHHHHHHHCHHHCCCCEEEEECCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHC
GKMERGKEITVHLAGGDLMIAWTEEGNVLMKGPAEVICHGVYEYKIEA
CHHHCCCEEEEEEECCCEEEEEECCCCEEEECCHHHHEECEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA