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The map label for this gene is splB

Identifier: 49480936

GI number: 49480936

Start: 4023025

End: 4024050

Strand: Reverse

Name: splB

Synonym: BT9727_3948

Alternate gene names: 49480936

Gene position: 4024050-4023025 (Counterclockwise)

Preceding gene: 49478581

Following gene: 49478577

Centisome position: 76.83

GC content: 37.72

Gene sequence:

>1026_bases
ATGAAACCGTTTATGCCAAAACTTGTTTACTTTGAACCGAAGGCACTTGAATATCCACTTGGAAAAGAGCTGTATGAGAA
GTTTACGAAGATGGGATTAGAAATTCGTGAAACGACATCCCATAATCAAATTCGAAATTTGCCGGGTGAAAATGATTTGC
AAAAGTATCGTAATGCAAAAGCAACGCTTGTCGTTGGGGTGAGGAAGACATTAAAGTTTGATACGTCAAAACCGTCAGCT
GAATATGCAATTCCGCTTGCAACAGGATGTATGGGACATTGTCATTATTGTTACTTGCAAACGACACTTGGGAGTAAGCC
TTATGTTCGCGTGTATGTGAATCTTGATGAAATATTTGAGAAGGCACAGCAATATATGGATGAAAGAGCACCTGAAATAA
CAAGGTTTGAAGCTGCTTGTACATCAGATATCGTTGGGATTGATCATTTAACACATGCATTAAAGCGCGCGATCGAATTC
ATTGGAGAAAGTGAGCATGGGCGTTTACGTTTTGTTACGAAATATTCGCACGTTGATCATTTATTAGATGCAAAACATAA
CGGGAAAACTCGTTTCAGGTTTAGTATTAATTCACGTTATGTAATTAAAAATTTTGAACCAGGGACATCACCGTTTGAAG
AAAGAATTGAAGCTGCTCGTAAAGTAGCGGACGCGGGTTATCCACTTGGATTTATAGTGGCGCCGCTTTATATGCATGAA
GGATGGGAAGAAGGATATCGTGAACTATTTGAGCGACTGTACAATGCATTAAACGATTTGTCGATACCGAATTTAACGTT
TGAACTAATTCAACATCGCTTTACAAAGCCAGCAAAAAAGGTTATTCAAGAGCGTTATCCGAATACGAAGCTTGAAATGG
ATGAAGAGAAGCGTAAATATAAATGGGGACGATATGGCATTGGGAAATACGTATATAAAAAAGATGATGCGGAAGTATTG
GAAGAAACGATAAGAGGTTATATATATGAGTTTTTTCCTGATGCAGAAATACAATACTTTACTTAA

Upstream 100 bases:

>100_bases
TATAACATTCAGTAACTTGCAACGCAAGTTACTGAAGTGGAAAACTTTAATGCGTGTTAATTTTTCATCGTTTCCCATAC
TAACGAGAGGAGGGAAGCAT

Downstream 100 bases:

>100_bases
GAGGAGTCTGTATGCAAATACAGATTCTTTTTTGTTTTATATATTTAGGCTTGTTTTCAACTTGTCACGTTATTCTTTCT
GGTGTATCATTTTATTGATT

Product: spore photoproduct lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKPFMPKLVYFEPKALEYPLGKELYEKFTKMGLEIRETTSHNQIRNLPGENDLQKYRNAKATLVVGVRKTLKFDTSKPSA
EYAIPLATGCMGHCHYCYLQTTLGSKPYVRVYVNLDEIFEKAQQYMDERAPEITRFEAACTSDIVGIDHLTHALKRAIEF
IGESEHGRLRFVTKYSHVDHLLDAKHNGKTRFRFSINSRYVIKNFEPGTSPFEERIEAARKVADAGYPLGFIVAPLYMHE
GWEEGYRELFERLYNALNDLSIPNLTFELIQHRFTKPAKKVIQERYPNTKLEMDEEKRKYKWGRYGIGKYVYKKDDAEVL
EETIRGYIYEFFPDAEIQYFT

Sequences:

>Translated_341_residues
MKPFMPKLVYFEPKALEYPLGKELYEKFTKMGLEIRETTSHNQIRNLPGENDLQKYRNAKATLVVGVRKTLKFDTSKPSA
EYAIPLATGCMGHCHYCYLQTTLGSKPYVRVYVNLDEIFEKAQQYMDERAPEITRFEAACTSDIVGIDHLTHALKRAIEF
IGESEHGRLRFVTKYSHVDHLLDAKHNGKTRFRFSINSRYVIKNFEPGTSPFEERIEAARKVADAGYPLGFIVAPLYMHE
GWEEGYRELFERLYNALNDLSIPNLTFELIQHRFTKPAKKVIQERYPNTKLEMDEEKRKYKWGRYGIGKYVYKKDDAEVL
EETIRGYIYEFFPDAEIQYFT
>Mature_341_residues
MKPFMPKLVYFEPKALEYPLGKELYEKFTKMGLEIRETTSHNQIRNLPGENDLQKYRNAKATLVVGVRKTLKFDTSKPSA
EYAIPLATGCMGHCHYCYLQTTLGSKPYVRVYVNLDEIFEKAQQYMDERAPEITRFEAACTSDIVGIDHLTHALKRAIEF
IGESEHGRLRFVTKYSHVDHLLDAKHNGKTRFRFSINSRYVIKNFEPGTSPFEERIEAARKVADAGYPLGFIVAPLYMHE
GWEEGYRELFERLYNALNDLSIPNLTFELIQHRFTKPAKKVIQERYPNTKLEMDEEKRKYKWGRYGIGKYVYKKDDAEVL
EETIRGYIYEFFPDAEIQYFT

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39974; Mature: 39974

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFMPKLVYFEPKALEYPLGKELYEKFTKMGLEIRETTSHNQIRNLPGENDLQKYRNAK
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHCCCCCHHHHHHCCCC
ATLVVGVRKTLKFDTSKPSAEYAIPLATGCMGHCHYCYLQTTLGSKPYVRVYVNLDEIFE
EEEEEEEHHHHEECCCCCCCCEEEEHHHCCCCCCEEEEEEECCCCCCEEEEEECHHHHHH
KAQQYMDERAPEITRFEAACTSDIVGIDHLTHALKRAIEFIGESEHGRLRFVTKYSHVDH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHH
LLDAKHNGKTRFRFSINSRYVIKNFEPGTSPFEERIEAARKVADAGYPLGFIVAPLYMHE
HHCCCCCCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GWEEGYRELFERLYNALNDLSIPNLTFELIQHRFTKPAKKVIQERYPNTKLEMDEEKRKY
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHH
KWGRYGIGKYVYKKDDAEVLEETIRGYIYEFFPDAEIQYFT
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MKPFMPKLVYFEPKALEYPLGKELYEKFTKMGLEIRETTSHNQIRNLPGENDLQKYRNAK
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHCCCCCHHHHHHCCCC
ATLVVGVRKTLKFDTSKPSAEYAIPLATGCMGHCHYCYLQTTLGSKPYVRVYVNLDEIFE
EEEEEEEHHHHEECCCCCCCCEEEEHHHCCCCCCEEEEEEECCCCCCEEEEEECHHHHHH
KAQQYMDERAPEITRFEAACTSDIVGIDHLTHALKRAIEFIGESEHGRLRFVTKYSHVDH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHH
LLDAKHNGKTRFRFSINSRYVIKNFEPGTSPFEERIEAARKVADAGYPLGFIVAPLYMHE
HHCCCCCCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GWEEGYRELFERLYNALNDLSIPNLTFELIQHRFTKPAKKVIQERYPNTKLEMDEEKRKY
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHH
KWGRYGIGKYVYKKDDAEVLEETIRGYIYEFFPDAEIQYFT
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA