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The map label for this gene is gnuT [H]

Identifier: 49479975

GI number: 49479975

Start: 3229634

End: 3230959

Strand: Reverse

Name: gnuT [H]

Synonym: BT9727_3162

Alternate gene names: 49479975

Gene position: 3230959-3229634 (Counterclockwise)

Preceding gene: 49479006

Following gene: 49481705

Centisome position: 61.69

GC content: 36.43

Gene sequence:

>1326_bases
ATGAATATGTACCTTTTAATGATTACATTATTATCAATTGTAATTGTTATTTTAGGGGTATCGTGGTGGAGATGGCATGC
GTTTATTAGTTTAACGATTGCAAGTTTGTTTTTAGCTATTATGTCTGGAATGCCAATGGATAAAATAGTGAGTGCTTATG
AAACGGGAGTCGGAAGTGTTCTTGGGCATTTAGTAGGTATTTTAGTATTAGGTACAATTTTAGGGAAAATGATGGCAGAG
TCAGGTGCTGGAATGCAAGTGGCAGAGTTCTTTATTAAATCCTTCGGAATAAAAAACTTACCATGGGCGATGTTAATTGC
GGGTTTTATTATTGGAATTCCAGTATTTTTTGAAGTAGGCATTTTAATATTATTGCCGTTAGTAATTTCTATTCATAAAA
CAACGAAACAAAATATTTTATTGATAGCATTACCAGCAATTGCTGGGTTATCTGTCGTACACGGCCTTGTTCCTCCGCAT
CCAGGGGCAATCGTTGCAATAGGTATTTATAAAGCGAATCTTGGGAAGGTATTATTGTATTCACTCATTATTACATTTTT
TGCAGCGATTATTGCAGGTCCTATATTCGCTAAATGGATTCACAAACGTGTAATTCCTGAAAATGAGCCAGAATTGATTC
GTGTAAATACAAAATCAACGAAATTACCAGGAATAGGAGTTTCGTTTTTAGTTATATTGTTACCTGTAATGTTAATGGTG
TTAGCAGCAGCAGCTCCTTATGTTCCTCTTTCTACTACCTTGATGAAGATTGTCACATTAATCGGTAGTCCTATCATTGC
ACTTTTGATTGCATGTTTTGCAGCGTTTTATTTTCTTGGATTTCGTCAAGGTATCGATAAAAAGGCGATTAAGAAGGTAA
CAGAAGATAGCTTATTACCAATCGGTTCAATTGTTGCGATTATTGGTGCTGGCGGCGGATTTAAGCAAATTCTTATCGAT
AGTGGTGTAGGGAATGCTATTGCTCAAATGTCTGAACATTTAGCGCTATCTCCAATTGTATTAGCATTTATGGTAGCTGG
ATTAATTCGAATTGCGACAGGGTCAGCGACAGTCGCCTTAACAACAGCAGCGGGTATCGTTTCGCCAATCGTTGAAAATA
CGACAGGTGTGAATGTAGAATTACTCGTCATTGCAACGGGTGCAGGAGCATTAATGTTTTCTCACGTAAACGATGCTGGG
TTCTGGATGGTAAAAGAATACCTAGGTTTAACAGTGAAAGAAACATTTAAAACATGGACAGTATTAGAAACATTACTTTC
ATTTATCGCTTTTGGAGGAGTTTTACTACTCGATATGTTTATTTAA

Upstream 100 bases:

>100_bases
GTATATCGATATATAAATTTTTACTCAAAAAAGAACTATATAGGTTCTTTTCATTGTATATCATTTTTGAAAGGGTTTAC
ATAAAGGGGTGGAATTGCGT

Downstream 100 bases:

>100_bases
AATAAGGATGTTTTTATGGTGGGATACTGTAGTAATTTTTTGTTAGGAGGAAGAAATGGTGAGTTCAAAATATATGATTG
GTGTAGACATTGGGACAACT

Product: gluconate permease

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNMYLLMITLLSIVIVILGVSWWRWHAFISLTIASLFLAIMSGMPMDKIVSAYETGVGSVLGHLVGILVLGTILGKMMAE
SGAGMQVAEFFIKSFGIKNLPWAMLIAGFIIGIPVFFEVGILILLPLVISIHKTTKQNILLIALPAIAGLSVVHGLVPPH
PGAIVAIGIYKANLGKVLLYSLIITFFAAIIAGPIFAKWIHKRVIPENEPELIRVNTKSTKLPGIGVSFLVILLPVMLMV
LAAAAPYVPLSTTLMKIVTLIGSPIIALLIACFAAFYFLGFRQGIDKKAIKKVTEDSLLPIGSIVAIIGAGGGFKQILID
SGVGNAIAQMSEHLALSPIVLAFMVAGLIRIATGSATVALTTAAGIVSPIVENTTGVNVELLVIATGAGALMFSHVNDAG
FWMVKEYLGLTVKETFKTWTVLETLLSFIAFGGVLLLDMFI

Sequences:

>Translated_441_residues
MNMYLLMITLLSIVIVILGVSWWRWHAFISLTIASLFLAIMSGMPMDKIVSAYETGVGSVLGHLVGILVLGTILGKMMAE
SGAGMQVAEFFIKSFGIKNLPWAMLIAGFIIGIPVFFEVGILILLPLVISIHKTTKQNILLIALPAIAGLSVVHGLVPPH
PGAIVAIGIYKANLGKVLLYSLIITFFAAIIAGPIFAKWIHKRVIPENEPELIRVNTKSTKLPGIGVSFLVILLPVMLMV
LAAAAPYVPLSTTLMKIVTLIGSPIIALLIACFAAFYFLGFRQGIDKKAIKKVTEDSLLPIGSIVAIIGAGGGFKQILID
SGVGNAIAQMSEHLALSPIVLAFMVAGLIRIATGSATVALTTAAGIVSPIVENTTGVNVELLVIATGAGALMFSHVNDAG
FWMVKEYLGLTVKETFKTWTVLETLLSFIAFGGVLLLDMFI
>Mature_441_residues
MNMYLLMITLLSIVIVILGVSWWRWHAFISLTIASLFLAIMSGMPMDKIVSAYETGVGSVLGHLVGILVLGTILGKMMAE
SGAGMQVAEFFIKSFGIKNLPWAMLIAGFIIGIPVFFEVGILILLPLVISIHKTTKQNILLIALPAIAGLSVVHGLVPPH
PGAIVAIGIYKANLGKVLLYSLIITFFAAIIAGPIFAKWIHKRVIPENEPELIRVNTKSTKLPGIGVSFLVILLPVMLMV
LAAAAPYVPLSTTLMKIVTLIGSPIIALLIACFAAFYFLGFRQGIDKKAIKKVTEDSLLPIGSIVAIIGAGGGFKQILID
SGVGNAIAQMSEHLALSPIVLAFMVAGLIRIATGSATVALTTAAGIVSPIVENTTGVNVELLVIATGAGALMFSHVNDAG
FWMVKEYLGLTVKETFKTWTVLETLLSFIAFGGVLLLDMFI

Specific function: Part Of The Gluconate Utilization System Gnt-I; High- Affinity Intake Of Gluconate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=440, Percent_Identity=39.7727272727273, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1790716, Length=421, Percent_Identity=40.3800475059382, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI2367370, Length=414, Percent_Identity=37.43961352657, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788707, Length=443, Percent_Identity=37.2460496613996, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1790776, Length=425, Percent_Identity=35.0588235294118, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI48994939, Length=433, Percent_Identity=36.7205542725173, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1789097, Length=444, Percent_Identity=33.5585585585586, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46873; Mature: 46873

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMYLLMITLLSIVIVILGVSWWRWHAFISLTIASLFLAIMSGMPMDKIVSAYETGVGSV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LGHLVGILVLGTILGKMMAESGAGMQVAEFFIKSFGIKNLPWAMLIAGFIIGIPVFFEVG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ILILLPLVISIHKTTKQNILLIALPAIAGLSVVHGLVPPHPGAIVAIGIYKANLGKVLLY
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHH
SLIITFFAAIIAGPIFAKWIHKRVIPENEPELIRVNTKSTKLPGIGVSFLVILLPVMLMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LAAAAPYVPLSTTLMKIVTLIGSPIIALLIACFAAFYFLGFRQGIDKKAIKKVTEDSLLP
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
IGSIVAIIGAGGGFKQILIDSGVGNAIAQMSEHLALSPIVLAFMVAGLIRIATGSATVAL
HHHHHHHHCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TTAAGIVSPIVENTTGVNVELLVIATGAGALMFSHVNDAGFWMVKEYLGLTVKETFKTWT
HHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
VLETLLSFIAFGGVLLLDMFI
HHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNMYLLMITLLSIVIVILGVSWWRWHAFISLTIASLFLAIMSGMPMDKIVSAYETGVGSV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LGHLVGILVLGTILGKMMAESGAGMQVAEFFIKSFGIKNLPWAMLIAGFIIGIPVFFEVG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ILILLPLVISIHKTTKQNILLIALPAIAGLSVVHGLVPPHPGAIVAIGIYKANLGKVLLY
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHH
SLIITFFAAIIAGPIFAKWIHKRVIPENEPELIRVNTKSTKLPGIGVSFLVILLPVMLMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LAAAAPYVPLSTTLMKIVTLIGSPIIALLIACFAAFYFLGFRQGIDKKAIKKVTEDSLLP
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
IGSIVAIIGAGGGFKQILIDSGVGNAIAQMSEHLALSPIVLAFMVAGLIRIATGSATVAL
HHHHHHHHCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TTAAGIVSPIVENTTGVNVELLVIATGAGALMFSHVNDAGFWMVKEYLGLTVKETFKTWT
HHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
VLETLLSFIAFGGVLLLDMFI
HHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]