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The map label for this gene is besA [H]

Identifier: 49479432

GI number: 49479432

Start: 3563094

End: 3563921

Strand: Reverse

Name: besA [H]

Synonym: BT9727_3479

Alternate gene names: 49479432

Gene position: 3563921-3563094 (Counterclockwise)

Preceding gene: 49478350

Following gene: 49479431

Centisome position: 68.04

GC content: 35.27

Gene sequence:

>828_bases
ATGAACACTACTATTGAAAAACAGCAGATTGTAACATCTAATACTGAACAGTGGAACATGTATACAAAATTAGATGGTAA
GGAATATCAAATTCATATTTCAAAGCCGAGGCAACCAGCACCGGATTCAGGATATCCTGTCATTTACGTATTAGATGGTA
ATGCCTTTTTTCAAACATTCCATGAAGCGGTTAAAATACAATCAGTTAGAGCAGAAAAAACAGGTGTTTCACCAGCCATT
ATAGTAGGTATTGGTTATCCAATCGAAGGGGCATTTTCAGGTGAAGAAAGATGCTATGATTTTACGCCTAGCGTAATTTC
AAAAGATGCGGCTTTAAAACCAGATGGTAAACCTTGGCCAAAAACAGGAGGAGCACATCATTTCTTTACTTTTATTGAAG
AAGAATTAAAGCCGCAAATTGAAAGGGATTTTGAAATTGATAAAGGAAAACAAACATTATTTGGGCATTCACTAGGTGGT
CTATTTGCTTTACACATATTATTTACAAACGTAAATGCCTTTCAAAATTATTTTATAAGTAGTCCATCTATTTGGTGGAA
TAACCAATCTGTGCTTGAAAAAGAAGGAAATCTTATAAATGAATTAAACAATGCAAAGGTTAAAACAAGGGTATTTCTTA
CAGTTGGTTCACTAGAACGAGAGCATATGGTTGTAGGTGCTAATGAATTGTCAGAGCGCCTTCTCCAAGTAAAACACGAT
CAGTTTCGGTTTACGTTTTATGAGGCTGAAGGGGAGAATCATGCATCAGTTGTGCCGAGTTCTTTAAGTAAAGGATTGAG
ATTCATTAGTCAAGTATCCATCGATTAA

Upstream 100 bases:

>100_bases
CCGTATAATGGGTCTCCTGTTTGCTTCGGTTATGATAGCGTTGTATCTCAAGAAGAAAAAGTAGAAATATATGTAACAAG
TTAAAAGGGGGGAATATAAA

Downstream 100 bases:

>100_bases
ATGGTTAGTGATTTGAGTGAATCCACATTTTTCTATGATCTGTAGTAGGTTAGATAATAAAACGATTGTTTAATAAAGTG
CAAACAAAAGGCTATGGAGA

Product: esterase

Products: NA

Alternate protein names: Bacillibactin trilactone hydrolase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNTTIEKQQIVTSNTEQWNMYTKLDGKEYQIHISKPRQPAPDSGYPVIYVLDGNAFFQTFHEAVKIQSVRAEKTGVSPAI
IVGIGYPIEGAFSGEERCYDFTPSVISKDAALKPDGKPWPKTGGAHHFFTFIEEELKPQIERDFEIDKGKQTLFGHSLGG
LFALHILFTNVNAFQNYFISSPSIWWNNQSVLEKEGNLINELNNAKVKTRVFLTVGSLEREHMVVGANELSERLLQVKHD
QFRFTFYEAEGENHASVVPSSLSKGLRFISQVSID

Sequences:

>Translated_275_residues
MNTTIEKQQIVTSNTEQWNMYTKLDGKEYQIHISKPRQPAPDSGYPVIYVLDGNAFFQTFHEAVKIQSVRAEKTGVSPAI
IVGIGYPIEGAFSGEERCYDFTPSVISKDAALKPDGKPWPKTGGAHHFFTFIEEELKPQIERDFEIDKGKQTLFGHSLGG
LFALHILFTNVNAFQNYFISSPSIWWNNQSVLEKEGNLINELNNAKVKTRVFLTVGSLEREHMVVGANELSERLLQVKHD
QFRFTFYEAEGENHASVVPSSLSKGLRFISQVSID
>Mature_275_residues
MNTTIEKQQIVTSNTEQWNMYTKLDGKEYQIHISKPRQPAPDSGYPVIYVLDGNAFFQTFHEAVKIQSVRAEKTGVSPAI
IVGIGYPIEGAFSGEERCYDFTPSVISKDAALKPDGKPWPKTGGAHHFFTFIEEELKPQIERDFEIDKGKQTLFGHSLGG
LFALHILFTNVNAFQNYFISSPSIWWNNQSVLEKEGNLINELNNAKVKTRVFLTVGSLEREHMVVGANELSERLLQVKHD
QFRFTFYEAEGENHASVVPSSLSKGLRFISQVSID

Specific function: Catalyzes the hydrolysis of the trilactone cycle of ferri-bacillibactin (ferri-BB) complex, leading to the formation of bacillibactin monomers and to cytosolic iron release, thus making iron available for metabolic use. Can also hydrolyze bacillibactin (B

COG id: COG2819

COG function: function code R; Predicted hydrolase of the alpha/beta superfamily

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: NA

Molecular weight: Translated: 31046; Mature: 31046

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00049 RIBOSOMAL_L14

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTIEKQQIVTSNTEQWNMYTKLDGKEYQIHISKPRQPAPDSGYPVIYVLDGNAFFQTF
CCCCCCCHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHH
HEAVKIQSVRAEKTGVSPAIIVGIGYPIEGAFSGEERCYDFTPSVISKDAALKPDGKPWP
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCC
KTGGAHHFFTFIEEELKPQIERDFEIDKGKQTLFGHSLGGLFALHILFTNVNAFQNYFIS
CCCCCHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
SPSIWWNNQSVLEKEGNLINELNNAKVKTRVFLTVGSLEREHMVVGANELSERLLQVKHD
CCEEEECCCHHHHHCCCHHHHCCCCEEEEEEEEEECCCCCCEEEECHHHHHHHHHHHCCC
QFRFTFYEAEGENHASVVPSSLSKGLRFISQVSID
CEEEEEEECCCCCCHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTTIEKQQIVTSNTEQWNMYTKLDGKEYQIHISKPRQPAPDSGYPVIYVLDGNAFFQTF
CCCCCCCHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHH
HEAVKIQSVRAEKTGVSPAIIVGIGYPIEGAFSGEERCYDFTPSVISKDAALKPDGKPWP
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCC
KTGGAHHFFTFIEEELKPQIERDFEIDKGKQTLFGHSLGGLFALHILFTNVNAFQNYFIS
CCCCCHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
SPSIWWNNQSVLEKEGNLINELNNAKVKTRVFLTVGSLEREHMVVGANELSERLLQVKHD
CCEEEECCCHHHHHCCCHHHHCCCCEEEEEEEEEECCCCCCEEEECHHHHHHHHHHHCCC
QFRFTFYEAEGENHASVVPSSLSKGLRFISQVSID
CEEEEEEECCCCCCHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]