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The map label for this gene is murD

Identifier: 49479386

GI number: 49479386

Start: 3752817

End: 3754169

Strand: Reverse

Name: murD

Synonym: BT9727_3654

Alternate gene names: 49479386

Gene position: 3754169-3752817 (Counterclockwise)

Preceding gene: 49478446

Following gene: 49478445

Centisome position: 71.68

GC content: 34.96

Gene sequence:

>1353_bases
TTGAAAACTGTAACTGAATTTCAAAATAAAAATATTCTTGTATTAGGCATTGCAAAAAGTGGTTATGCAGCAGCTACGCT
GTTACAAAAGTTAGGGGCAAATGTTATTGTAAATGACGGAAAGCCGTTAGCAGAAAATGTACTCGCTGCAGAATTACAAG
CAAAAGGAATGGACGTTGTATGCGGTGGTCATCCTTTAGAATTATTAGAGAGAAATATTTCTCTTGTAGTAAAAAATCCA
GGAATCCCGTATTCTAATCCAATATTAGTTGCTGCGAAAGAAAAGCAAATTCCAATTGTTACAGAAGTTGAATTAGCATA
TCGTATTTCAGAAGCACCATTTGTTGGGATTACGGGATCTAACGGTAAAACGACGACGACAATGCTTACATTTGAAATGC
TAAAAGAAGGACAGAAGCATCCGGTAATTGCAGGGAACATTGGAACTGTAGCGTGTGAAGTTGCACAGGATGCAAAAGAA
AATGAAGTTGTAGTTACAGAACTTTCATCGTTCCAATTGATGGGAGTAGAATTGTTCCAACCTAAAATTGCTGCGTTTTT
AAATTTATTTGAGGCCCACTTAGATTATCATGGGACGAAGAAAGAATATGGTTTAGCAAAAGCAAATATTTTTAAAAACC
AAACAGAAAATGATTATAGTGTAATAAATGCAGATGATGCAGATGTGATGGCTTTATCTGCTTATAGTAAAGGTCAAAAA
GTACTATTCTCAACAACGAAAGAAATTGAAGATGGTGCGTGTATAAAAGATAATGCTCTTTATTTTAAAGCTGAAAAAGT
TGTTGAAGTAGACGATATCGTTTTACCTGGACAACATAATTTAGAAAATATTTTAGCAGCGATGAGTATCGCAAAATTAT
TGGGTGTTTCTAACGAAGCGATTACGGCGGTGTTAAAACGTTTTACAGGTGTAAAACATCGTTTAGAATATGTAACAACT
ATAAACAACCGTAAGTTTTATAATGACTCAAAAGCAACGAATATGTTAGCGACAGAGAAAGCTTTATCTGCGTTTACACA
ACCGACTGTGTTATTAGCAGGTGGACTTGATCGTGGGAATGAGTTCGATGATTTAATTCCGTATTTCAAAAATGTTAAAG
CGATTGTAACATTTGGACAAACAGCTCCAAAATTAGTAAGAGCTGCAGAAAAAGCAGGATTAGAGACAATTGAAAGTGTC
GATACTTTAGATGAAGCGGTAGTAAAAGCTTATGCTCATTCTACAGATGGCGATGTAATTCTTCTTTCTCCAGCATGTGC
AAGCTGGGATCAATTTAAAACATTTGAAGAAAGAGGAGACATTTTTATACAAGCTGTGCATAAACTTATATAA

Upstream 100 bases:

>100_bases
TGAATTATGTGGTTGGTCAGAGTGGCGTGTTGTTGTAACGTTTTGGTCTGTAGGATTTTTATTAGCTGTGTTAGGAATTT
ATATCGGGGTGTGGATGTAA

Downstream 100 bases:

>100_bases
AGTAAATCAAAACATCAGTAGGCTAACACCTACTGATGTTTTGTTACATAGGGCAAAAGTTTCTGCCTAAAGAGGTGGTG
TTTATGGGTAATGAAGAAAA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVVCGGHPLELLERNISLVVKNP
GIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGSNGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKE
NEVVVTELSSFQLMGVELFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTT
INNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLETIESV
DTLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI

Sequences:

>Translated_450_residues
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVVCGGHPLELLERNISLVVKNP
GIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGSNGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKE
NEVVVTELSSFQLMGVELFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTT
INNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLETIESV
DTLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI
>Mature_450_residues
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVVCGGHPLELLERNISLVVKNP
GIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGSNGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKE
NEVVVTELSSFQLMGVELFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTT
INNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLETIESV
DTLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48916; Mature: 48916

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV
CCCCHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS
ECCCHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEHEEEEECCCCEEEEECC
NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ
CCCEEEHEEEHHHHHCCCCCCEEECCCCCEEEEHHHCCCCCCEEEEECCCEEEECHHHHH
PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
HHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCCE
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEA
EEEEEHHCCCCCCEEECCEEEEEECCEEEECCEEECCCCCHHHHHHHHHHHHHHCCCHHH
ITAVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGN
HHHHHHHHHHHHHHHHHEEEECCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
EFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLETIESVDTLDEAVVKAYAHSTDGDVI
CHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLSPACASWDQFKTFEERGDIFIQAVHKLI
EECCCCCCHHHHHHHHHHCHHHHHHHHHHC
>Mature Secondary Structure
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV
CCCCHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS
ECCCHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEHEEEEECCCCEEEEECC
NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ
CCCEEEHEEEHHHHHCCCCCCEEECCCCCEEEEHHHCCCCCCEEEEECCCEEEECHHHHH
PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
HHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCCE
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEA
EEEEEHHCCCCCCEEECCEEEEEECCEEEECCEEECCCCCHHHHHHHHHHHHHHCCCHHH
ITAVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGN
HHHHHHHHHHHHHHHHHEEEECCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
EFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLETIESVDTLDEAVVKAYAHSTDGDVI
CHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLSPACASWDQFKTFEERGDIFIQAVHKLI
EECCCCCCHHHHHHHHHHCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA