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The map label for this gene is dapL [H]

Identifier: 49479380

GI number: 49479380

Start: 3818293

End: 3819423

Strand: Reverse

Name: dapL [H]

Synonym: BT9727_3722

Alternate gene names: 49479380

Gene position: 3819423-3818293 (Counterclockwise)

Preceding gene: 49478484

Following gene: 49479379

Centisome position: 72.92

GC content: 38.55

Gene sequence:

>1131_bases
ATGGCAGTAAGCAAATTCGTTCAAATTCGTAGAGACTTACACAAAATACCGGAAATTGGATTTGAAGAGTGGAAAACACA
ACAGTATATTTTAGATTACATAGGAACGCTTTTAAACGAACATGTGGAAGTGAAGGTATGGAGAACGGGTGTCATTGTTA
AAGTAAAGGGGAAAAATCCAGAAAAAGTTATAGGGTATCGCGCAGATATAGACGGTTTACCAATTACAGAGGAAACTGGA
TATGAGTTCGCTTCTGTTCATGAAGGAATGATGCATGCATGCGGACACGACTTACATACAACAATTGGTTTAGGCCTTCT
AACAGCAGCTGTAACAGAAAGAATAGATGATGACCTTGTATTTCTATTCCAACCAGCTGAAGAAGGGCCTGGAGGGGCTC
TTCCTATGTTAGAGAGCGAAGAGTTAAAAGAATGGAAGCCGAATATAATTCTCGGTCTTCATATTGCACCTGAATACCCT
GTAGGAACAATTGCTACAAAAGAGGGGCTATTATTTGCAAATACATCAGAATTATATGTTGATTTAAAGGGGAAAGGTGG
ACATGCAGCTTACCCTCATACTGCAAATGATATGATTGTCGCAGCTAGTCACCTTGTTACACAGCTTCAGTCCGTTATTA
GTCGTAATGTAAACCCACTTGATAGTGCAGTAATTACGATTGGGAAAATAACAGGCGGTACAGTTCAAAATATCATAGCA
GAAAAGTCTCGATTAGAGGGAACAATCCGAACGTTATCAGTGGAATCAATGAGCCGTGTTAAAAGTAGGATTGAGGCAAT
TGTTGCAGGTATAGAAGCTTCCTTCCAATGTGAGGCCGTTATTGACTACGGAGCGATGTATCATCAAGTATACAACCATG
AAGCATTAACGAGAGAGTTTATGCAATTTGTAAGTGAACAAACTGATATGAAAGTAATTACATGTACTGAGGCAATGACA
GGTGAAGATTTTGGTTATATGCTTCAAGAGATTCCTGGATTTATGTTTTGGCTTGGGGTAAATTCAGAATATGGTTTACA
TCACGCAAAATTAAAACCAGATGAAGAGGCTATTGAGAAAGCTATCGTATTTTTAGATCAATATGTGAAGTGGAAGGGAA
CTAGAAAGTAA

Upstream 100 bases:

>100_bases
ATTAAACAAGAGCTTCGTCAATTAAACCCAGAGAAATAAAAACATAAAAGCGTAAGAGCATAAACAGGCTCTTACGCTTT
TTATAGATAGAGGTGCAAAA

Downstream 100 bases:

>100_bases
ACTTGCTCGCCAACTATGTAACAATTTAAAGTTGGTATTTGTTAGTTTCAAGGTGAAAACATCTTTCATATTTGTTTTGG
ATACACATCGGCATACAAAT

Product: M20/M25/M40 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAVSKFVQIRRDLHKIPEIGFEEWKTQQYILDYIGTLLNEHVEVKVWRTGVIVKVKGKNPEKVIGYRADIDGLPITEETG
YEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYP
VGTIATKEGLLFANTSELYVDLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREFMQFVSEQTDMKVITCTEAMT
GEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLKPDEEAIEKAIVFLDQYVKWKGTRK

Sequences:

>Translated_376_residues
MAVSKFVQIRRDLHKIPEIGFEEWKTQQYILDYIGTLLNEHVEVKVWRTGVIVKVKGKNPEKVIGYRADIDGLPITEETG
YEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYP
VGTIATKEGLLFANTSELYVDLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREFMQFVSEQTDMKVITCTEAMT
GEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLKPDEEAIEKAIVFLDQYVKWKGTRK
>Mature_375_residues
AVSKFVQIRRDLHKIPEIGFEEWKTQQYILDYIGTLLNEHVEVKVWRTGVIVKVKGKNPEKVIGYRADIDGLPITEETGY
EFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYPV
GTIATKEGLLFANTSELYVDLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIAE
KSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREFMQFVSEQTDMKVITCTEAMTG
EDFGYMLQEIPGFMFWLGVNSEYGLHHAKLKPDEEAIEKAIVFLDQYVKWKGTRK

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=217, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.47 [H]

Molecular weight: Translated: 41896; Mature: 41764

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSKFVQIRRDLHKIPEIGFEEWKTQQYILDYIGTLLNEHVEVKVWRTGVIVKVKGKNP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEECCCCC
EKVIGYRADIDGLPITEETGYEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLV
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
FLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYPVGTIATKEGLLFANTSELYV
EEEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCEEEEECCEEEE
DLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHH
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREF
HHHHHCHHHEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MQFVSEQTDMKVITCTEAMTGEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLKPDEEAIEK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AIVFLDQYVKWKGTRK
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AVSKFVQIRRDLHKIPEIGFEEWKTQQYILDYIGTLLNEHVEVKVWRTGVIVKVKGKNP
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEECCCCC
EKVIGYRADIDGLPITEETGYEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLV
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
FLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYPVGTIATKEGLLFANTSELYV
EEEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCEEEEECCEEEE
DLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHH
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREF
HHHHHCHHHEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MQFVSEQTDMKVITCTEAMTGEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLKPDEEAIEK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AIVFLDQYVKWKGTRK
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA