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The map label for this gene is argH

Identifier: 49479274

GI number: 49479274

Start: 3380863

End: 3382371

Strand: Reverse

Name: argH

Synonym: BT9727_3308

Alternate gene names: 49479274

Gene position: 3382371-3380863 (Counterclockwise)

Preceding gene: 49479473

Following gene: 49479275

Centisome position: 64.58

GC content: 34.26

Gene sequence:

>1509_bases
ATGAAACAAAGTAAAGAAGAGTTTATAAAGAGTGAAGGCGCTGATTTCCCTGGGAAAACATACGTGAATTGCGTATTACA
ACATGTATTTAATTTTCAACGAAATTACTTATTGAAAGATATGTTTCAAGTGCATAAAGCGCATATTGTGATGTTGACGG
AAGAAAATTTAATGAAAAAGGAAGAAGCTAAATTTATATTACACGCATTAAAAAAGGTTGAGGAAATTCCAGAAGAGCAA
TTGCTCTATACAGAGCAACATGAAGATCTCTTTTTTTTAGTGGAACATTTAATTTCTCAAGAAGCGAAAAGCGATTTTGT
AAGTAATATGCATATTGGTAGAAGTAGAAATGATATGGGTGTTACGATGTATCGCATGAGTTTAAGGCGATATGTGCTAC
GATTAATGGAACATCATTTGTTATTGCAAGAAAGTATATTGCAACTTGCTGCTGATCATAAGGAAACGATTATGCCAGCT
TATACACATACACAACCAGCTCAGCCAACAACATTTGGTCATTATATGTTAGCGATTTATGATACGATGCAGAGAGATTT
AGAACGCATGAAAAAAACGTACAAACTTTTAAATCAATCGCCAATGGGAGCTGCTGCGCTTTCTACAACAAGTTTCCCAA
TTAAACGAGAGCGAGTTGCCGATTTACTTGGATTTACTAATGTAATTGAGAACTCATATGATGCTGTCGCTGGAGCGGAT
TATTTATTAGAGGTTAGCTCGTTACTTATGGTAATGATGACGAATACGAGTAGATGGATTCATGATTTCTTACTATTAGC
GACGAAAGAATATGATGGTATTACTATTGCAAGGCCGTATGTACAAATTAGTAGTATTATGCCACAAAAGCGAAATCCAG
TTTCGATTGAACACGCCCGTGCAATTACGAGTAGTGCTCTAGGGGAAGCATTTACAGTATTTCAAATGATTCATAATACA
CCATTTGGTGATATTGTCGATACGGAAGATGACTTGCAGCCGTATTTATATAAAGGAATTGAAAAGGCTATTCGTGTATT
TTGCATTTTGAATGCAGTCATTCGAACGATGAAAGTAGAAGAGGATACCTTAAAAAGACGTTCATATAAACATGCAATTA
CGATTACAGATTTCGCAGATGTTTTAACGAAAAACTATGGCATCCCATTTCGGCATGCTCATCATGCGGCAAGTGTTATT
GCGAATATGTCGTTGGAACAGAAAAAGGAGCTACATGAATTATGTTTCAAAGATGTAAATATATACTTACAGGAAAAATT
TAAAATACAATTATTAGAAAAAGAGTGGGAAGAAATTATATCGCCAGAAGCTTTTATACAAAAAAGAAATGTATATGGTG
GAACGAGTAAAAAAGAAATGGAGCGCATGATTAACAATCGAAAAGAGTTATTTCGAAAAGAAGAAGAGGTATTTGAACAG
GAAAAACAGAGGATTTTACAAGCTGAAAATGATTTGAATATGTTAGCTTCGAATATTATTGAATCGTAA

Upstream 100 bases:

>100_bases
CTCGGTGCAGAGAGCACCGAGTTTATTTTTGTGTACAAGCACTTTTTTCCTATCTATGGGTATGATGGGTAGTAATACTG
AGGTGAAAGGGGAAAAGGTG

Downstream 100 bases:

>100_bases
AAGAGGGATTTAATGAATTAATCGCCAATACTTAATAAAACTATAAATAGAGAGAAGAGATTGTCGATGATTCAATTACA
TGTATATGAAGAGGTTGTTA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL 2; Arginosuccinase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKQSKEEFIKSEGADFPGKTYVNCVLQHVFNFQRNYLLKDMFQVHKAHIVMLTEENLMKKEEAKFILHALKKVEEIPEEQ
LLYTEQHEDLFFLVEHLISQEAKSDFVSNMHIGRSRNDMGVTMYRMSLRRYVLRLMEHHLLLQESILQLAADHKETIMPA
YTHTQPAQPTTFGHYMLAIYDTMQRDLERMKKTYKLLNQSPMGAAALSTTSFPIKRERVADLLGFTNVIENSYDAVAGAD
YLLEVSSLLMVMMTNTSRWIHDFLLLATKEYDGITIARPYVQISSIMPQKRNPVSIEHARAITSSALGEAFTVFQMIHNT
PFGDIVDTEDDLQPYLYKGIEKAIRVFCILNAVIRTMKVEEDTLKRRSYKHAITITDFADVLTKNYGIPFRHAHHAASVI
ANMSLEQKKELHELCFKDVNIYLQEKFKIQLLEKEWEEIISPEAFIQKRNVYGGTSKKEMERMINNRKELFRKEEEVFEQ
EKQRILQAENDLNMLASNIIES

Sequences:

>Translated_502_residues
MKQSKEEFIKSEGADFPGKTYVNCVLQHVFNFQRNYLLKDMFQVHKAHIVMLTEENLMKKEEAKFILHALKKVEEIPEEQ
LLYTEQHEDLFFLVEHLISQEAKSDFVSNMHIGRSRNDMGVTMYRMSLRRYVLRLMEHHLLLQESILQLAADHKETIMPA
YTHTQPAQPTTFGHYMLAIYDTMQRDLERMKKTYKLLNQSPMGAAALSTTSFPIKRERVADLLGFTNVIENSYDAVAGAD
YLLEVSSLLMVMMTNTSRWIHDFLLLATKEYDGITIARPYVQISSIMPQKRNPVSIEHARAITSSALGEAFTVFQMIHNT
PFGDIVDTEDDLQPYLYKGIEKAIRVFCILNAVIRTMKVEEDTLKRRSYKHAITITDFADVLTKNYGIPFRHAHHAASVI
ANMSLEQKKELHELCFKDVNIYLQEKFKIQLLEKEWEEIISPEAFIQKRNVYGGTSKKEMERMINNRKELFRKEEEVFEQ
EKQRILQAENDLNMLASNIIES
>Mature_502_residues
MKQSKEEFIKSEGADFPGKTYVNCVLQHVFNFQRNYLLKDMFQVHKAHIVMLTEENLMKKEEAKFILHALKKVEEIPEEQ
LLYTEQHEDLFFLVEHLISQEAKSDFVSNMHIGRSRNDMGVTMYRMSLRRYVLRLMEHHLLLQESILQLAADHKETIMPA
YTHTQPAQPTTFGHYMLAIYDTMQRDLERMKKTYKLLNQSPMGAAALSTTSFPIKRERVADLLGFTNVIENSYDAVAGAD
YLLEVSSLLMVMMTNTSRWIHDFLLLATKEYDGITIARPYVQISSIMPQKRNPVSIEHARAITSSALGEAFTVFQMIHNT
PFGDIVDTEDDLQPYLYKGIEKAIRVFCILNAVIRTMKVEEDTLKRRSYKHAITITDFADVLTKNYGIPFRHAHHAASVI
ANMSLEQKKELHELCFKDVNIYLQEKFKIQLLEKEWEEIISPEAFIQKRNVYGGTSKKEMERMINNRKELFRKEEEVFEQ
EKQRILQAENDLNMLASNIIES

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=473, Percent_Identity=26.0042283298097, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI68303542, Length=473, Percent_Identity=26.0042283298097, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI68303547, Length=473, Percent_Identity=26.215644820296, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI68303549, Length=473, Percent_Identity=24.3128964059197, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790398, Length=417, Percent_Identity=28.7769784172662, Blast_Score=180, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321806, Length=473, Percent_Identity=27.2727272727273, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI221473854, Length=425, Percent_Identity=26.5882352941176, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI78706858, Length=425, Percent_Identity=26.5882352941176, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF00206 Lyase_1 [H]

EC number: =4.3.2.1 [H]

Molecular weight: Translated: 58522; Mature: 58522

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKEEFIKSEGADFPGKTYVNCVLQHVFNFQRNYLLKDMFQVHKAHIVMLTEENLMKK
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHH
EEAKFILHALKKVEEIPEEQLLYTEQHEDLFFLVEHLISQEAKSDFVSNMHIGRSRNDMG
HHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
VTMYRMSLRRYVLRLMEHHLLLQESILQLAADHKETIMPAYTHTQPAQPTTFGHYMLAIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHH
DTMQRDLERMKKTYKLLNQSPMGAAALSTTSFPIKRERVADLLGFTNVIENSYDAVAGAD
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
YLLEVSSLLMVMMTNTSRWIHDFLLLATKEYDGITIARPYVQISSIMPQKRNPVSIEHAR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHH
AITSSALGEAFTVFQMIHNTPFGDIVDTEDDLQPYLYKGIEKAIRVFCILNAVIRTMKVE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDTLKRRSYKHAITITDFADVLTKNYGIPFRHAHHAASVIANMSLEQKKELHELCFKDVN
HHHHHHHHHCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IYLQEKFKIQLLEKEWEEIISPEAFIQKRNVYGGTSKKEMERMINNRKELFRKEEEVFEQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKQRILQAENDLNMLASNIIES
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSKEEFIKSEGADFPGKTYVNCVLQHVFNFQRNYLLKDMFQVHKAHIVMLTEENLMKK
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHH
EEAKFILHALKKVEEIPEEQLLYTEQHEDLFFLVEHLISQEAKSDFVSNMHIGRSRNDMG
HHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
VTMYRMSLRRYVLRLMEHHLLLQESILQLAADHKETIMPAYTHTQPAQPTTFGHYMLAIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHH
DTMQRDLERMKKTYKLLNQSPMGAAALSTTSFPIKRERVADLLGFTNVIENSYDAVAGAD
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
YLLEVSSLLMVMMTNTSRWIHDFLLLATKEYDGITIARPYVQISSIMPQKRNPVSIEHAR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHH
AITSSALGEAFTVFQMIHNTPFGDIVDTEDDLQPYLYKGIEKAIRVFCILNAVIRTMKVE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDTLKRRSYKHAITITDFADVLTKNYGIPFRHAHHAASVIANMSLEQKKELHELCFKDVN
HHHHHHHHHCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IYLQEKFKIQLLEKEWEEIISPEAFIQKRNVYGGTSKKEMERMINNRKELFRKEEEVFEQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKQRILQAENDLNMLASNIIES
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]