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The map label for this gene is rihA [H]

Identifier: 49479273

GI number: 49479273

Start: 3383699

End: 3384646

Strand: Reverse

Name: rihA [H]

Synonym: BT9727_3310

Alternate gene names: 49479273

Gene position: 3384646-3383699 (Counterclockwise)

Preceding gene: 49478272

Following gene: 49479473

Centisome position: 64.62

GC content: 36.5

Gene sequence:

>948_bases
ATGGAGTTGAGGGCGATGAAAAAAGTATTATTTTTAGGAGACCCAGGAATTGATGACTCTTTAGCAATTATGTATGGATT
GTTGCATCCTGATATTGATATTGTTGGTGTAGTAACTGGATATGGAAATGTAACGCAAGAAAAGGCGACAAGTAATGCGG
CATATTTATTGCAACTGGCAGGACGGGAAGATATACCTATTATTAATGGTGCGAAAATCCCTTTATCTGGAGATATTACA
ACGTATTATCCAGAAATTCATGGGGCGGAAGGCTTAGGACCAATTCGACCGCCGAAAAATCTTTCTCCAAATATAAGACC
TTTTTGTGAGTTTTTTGACATTCTTGAAAAATATAAAGGAGAATTAATTATAGTTGATGCTGGGAGGTCAACGACACTTG
CAACAGCATTTATTTTAGAAAAACCATTGATGAAGTATGTGAAAGAATATTATATAATGGGCGGTGCTTTTTTAATGCCT
GGAAATGTTACACCAGTCGCAGAAGCGAATTTTCATGGTGACCCTATTGCATCACAATTAGTCATGCAAAATGCCAAGAA
TGTGACGTTGGTGCCGCTGAATGTTACATCTGAAGCTATAATCACGCCAGAGATGGTAAAGTACATTACGAAACATTCTA
AAACGAGTTTTAATAAATTAATTGAACCGATTTTTACGTATTATTATAAAGCTTATAGAAAGTTAAATCCGAAAATAACA
GGAAGTCCGGTACATGACGTTGTTACAATGATGGTCGCGGCGAATCCTTCAATATTGGATTATGTGTATCGTCGTGTAGA
TGTAGATACAGTGGGGATTGCAAAAGGAGAAAGTATTGCAGATTTCCGTCCTCAACCTGATGCAAAAGCCTTAAAAAATT
GGGTACGAATTGGTTGGTCATTACATTATAAAAAATTCCTTGAGGATTTTGTGAAAATCATGACGTAG

Upstream 100 bases:

>100_bases
TGAAAAACGTAGTCCTTACAGGACTGCGTTTTTTCGTATAAATTTGTTCTTTTTATTCTGTAATCCTGTGAATATTCATA
AATTAATAACAATTGAGGGG

Downstream 100 bases:

>100_bases
ACATAATGGGAGTATACAAGTTAAAATAATGGAGATGGTTATTCAAATAACCATCTCCATTATTTTGTTTTTTTAAGATA
GAGAGAAAGAGTTACACAAT

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDIT
TYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMP
GNVTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKIT
GSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Sequences:

>Translated_315_residues
MELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDIT
TYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMP
GNVTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKIT
GSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT
>Mature_315_residues
MELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDIT
TYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMP
GNVTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKIT
GSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=280, Percent_Identity=29.2857142857143, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788486, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=28.4313725490196, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI32565474, Length=202, Percent_Identity=28.7128712871287, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565698, Length=200, Percent_Identity=29, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=275, Percent_Identity=30.5454545454545, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 35065; Mature: 35065

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLA
CCHHHHHHHHEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEEC
GREDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKG
CCCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
ELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHH
VMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKIT
HHHCCCCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWS
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LHYKKFLEDFVKIMT
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MELRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLA
CCHHHHHHHHEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEEC
GREDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKG
CCCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
ELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHH
VMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKIT
HHHCCCCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GSPVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWS
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LHYKKFLEDFVKIMT
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA