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The map label for this gene is amiB

Identifier: 49479019

GI number: 49479019

Start: 3116552

End: 3118327

Strand: Reverse

Name: amiB

Synonym: BT9727_3033

Alternate gene names: 49479019

Gene position: 3118327-3116552 (Counterclockwise)

Preceding gene: 49478108

Following gene: 49479509

Centisome position: 59.54

GC content: 37.84

Gene sequence:

>1776_bases
ATGAATATTAAAAAACGAGTAATCGCACTATCAACAAGTTTAGTGATTTTAGGGAGTGCACCTTTATCAATATCAGCAGA
AGAGATAAATAAATCTCATACTGACCAGCTTAGTATTGCACAAGGGGAACGTGGAACGAGCGGATTACGTTATTCTGATT
TCAATCTAGAAACTCCTGATCACATGCAAATTAACGAAGCGGAAGTATCAGGAAATGAGAAAATTCTTTTAGAAGAATAT
AAGCATAAATATTCTGAATCAGAAGAATTACATAAAATGAAAGAAGCGAAAAAAGGCCAAGTAGCGGAAGAAGAAGCTAT
TTCAAACATTGAAAAAGCTTTTGAACATGTAGACGGTCGAAATGATGGTTCTACTCGCGAAATGAAGCCGGTTGAAAAAC
GGAGAGCTATTCAGTATAGCGCATCGATGACTACGAAGAAATATGGAAAACTTGCAGGTACTCCATTTGTAGAGTGGATT
GTTCCAGCAGGAAACCCTGATATTCGCCCCGCTAATCCGATGAAAGCACGTTATATTACAATTCACGAAACTGCAAATAC
TGCCCGCGGTGCAAATGCTGAAAACCATGCGAAGTATTTATATAAACAAGCAACGGAAGGTACGTTCCGAACAGCGTCAT
GGCATTTTACTGTAGACGATAAACAAATTTATCAGCACTTACCAACAAATGAAAATGGTTGGCATGCAGGAGATGGTGAT
GGTTCAGGAAATAGAGAATCTATCGGTATTGAAATTTCAGTGAATCAAGATGGGGATTACAATAAAGCGTTAGAGAATGC
AAAGAGACTTGCGGGATATTTAATGAACAAAGAAGGAATTAGCGCGGACCATATTTATAGACATCAACAATGGAGCGGGA
AAAACTGTCCTGATATTTTAATTTCTCGTGGGAATTGGACTGGCTTCGTACAAGGTATTCAATGGTATGCAAATGTTAAC
GCACAAATTGATTCGCCTTTACAAGAAAGAAATGAGCTATTTGATTCAAATGAATATAATCCAGCTGAACCTAATATGGA
GCTTGTAGTAAAAGGGGATGGTATAAACGTACGTAGTGGTGCAGGACTGGAACATCAAACTGTGCGTAAAGCTTCAAATG
GAGACCGTTACAAAGTGTTAGCAGTTAAAAACGGTTGGTATAAAGTAGGTAATGATGAGTGGATTTTCTATAACCCAAGT
TGGATTAAAATAAACTATAATGTGCCAAATAAAGAAGTACAAAAACCAAAAGATGATATTACAGGTGGATGGTTTGAAGG
GCATATTCGCAAGCTGAACAGCCTAGGAATTATGTACGGGGAAGGAAATGGTGTATTTGCGCCTTATCGTAATGTAACGA
GAGCTGAGTTTGCGACATTAATTTCAAATGCTCTTAAACTACCAGAAGGAAATAAGACATTTGTAGACATTAACAAAGCG
CATCCGTCACTTCATAATGGTATTAAACGTAGTGCAAGCGCAGGAATTATTAACGGTCGTGGCGGCGGGATTTTTGATCC
TAACGCATCTATCACTCGTGAAGAAGCATCGATCATGATTGACAAAGCATTACAGTATAAAGGTGTTACAGGTGAATTAG
CCGCATTACCATTTACAGATCAACATTTAATTACATACAAACAGCATGTTCAACGATTATATAGCTTAAAGGTAGTTACT
GGTGTTGGAAATAATGAGTTTAATCCTAAAGGGACGACAACTCGTGGTGAAGCAGCTGCCTTTTTAGTAAAGATGTTAGA
TGCAAGTCAAAAATAG

Upstream 100 bases:

>100_bases
AACATATTTTCATACATAGTAGATTCATAGTCTTTCACGAATGAGAAAATAAAGGGAGGTTGAGATATTTACACGGATAG
TGTAGGTATCGATTACAGCA

Downstream 100 bases:

>100_bases
AAAGAATGATAAAGGAGCTATCCAAAATGAGAATTTGGAACAGCTCCTTTTTATATAAGCCCTTATTAAGCCCCTTACAA
GCCCCTTATAAGCACCTACC

Product: N-acetylmuramoyl-L-alanine amidase and S-layer protein fusion

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHTDQLSIAQGERGTSGLRYSDFNLETPDHMQINEAEVSGNEKILLEEY
KHKYSESEELHKMKEAKKGQVAEEEAISNIEKAFEHVDGRNDGSTREMKPVEKRRAIQYSASMTTKKYGKLAGTPFVEWI
VPAGNPDIRPANPMKARYITIHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDILISRGNWTGFVQGIQWYANVN
AQIDSPLQERNELFDSNEYNPAEPNMELVVKGDGINVRSGAGLEHQTVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPS
WIKINYNVPNKEVQKPKDDITGGWFEGHIRKLNSLGIMYGEGNGVFAPYRNVTRAEFATLISNALKLPEGNKTFVDINKA
HPSLHNGIKRSASAGIINGRGGGIFDPNASITREEASIMIDKALQYKGVTGELAALPFTDQHLITYKQHVQRLYSLKVVT
GVGNNEFNPKGTTTRGEAAAFLVKMLDASQK

Sequences:

>Translated_591_residues
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHTDQLSIAQGERGTSGLRYSDFNLETPDHMQINEAEVSGNEKILLEEY
KHKYSESEELHKMKEAKKGQVAEEEAISNIEKAFEHVDGRNDGSTREMKPVEKRRAIQYSASMTTKKYGKLAGTPFVEWI
VPAGNPDIRPANPMKARYITIHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDILISRGNWTGFVQGIQWYANVN
AQIDSPLQERNELFDSNEYNPAEPNMELVVKGDGINVRSGAGLEHQTVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPS
WIKINYNVPNKEVQKPKDDITGGWFEGHIRKLNSLGIMYGEGNGVFAPYRNVTRAEFATLISNALKLPEGNKTFVDINKA
HPSLHNGIKRSASAGIINGRGGGIFDPNASITREEASIMIDKALQYKGVTGELAALPFTDQHLITYKQHVQRLYSLKVVT
GVGNNEFNPKGTTTRGEAAAFLVKMLDASQK
>Mature_591_residues
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHTDQLSIAQGERGTSGLRYSDFNLETPDHMQINEAEVSGNEKILLEEY
KHKYSESEELHKMKEAKKGQVAEEEAISNIEKAFEHVDGRNDGSTREMKPVEKRRAIQYSASMTTKKYGKLAGTPFVEWI
VPAGNPDIRPANPMKARYITIHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDILISRGNWTGFVQGIQWYANVN
AQIDSPLQERNELFDSNEYNPAEPNMELVVKGDGINVRSGAGLEHQTVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPS
WIKINYNVPNKEVQKPKDDITGGWFEGHIRKLNSLGIMYGEGNGVFAPYRNVTRAEFATLISNALKLPEGNKTFVDINKA
HPSLHNGIKRSASAGIINGRGGGIFDPNASITREEASIMIDKALQYKGVTGELAALPFTDQHLITYKQHVQRLYSLKVVT
GVGNNEFNPKGTTTRGEAAAFLVKMLDASQK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 65800; Mature: 65800

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHTDQLSIAQGERGTSGLRYSDFNLETPD
CCCHHEEEEEEEEEEEEECCCCEEEHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCC
HMQINEAEVSGNEKILLEEYKHKYSESEELHKMKEAKKGQVAEEEAISNIEKAFEHVDGR
CCEEEHEECCCCCEEEHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
NDGSTREMKPVEKRRAIQYSASMTTKKYGKLAGTPFVEWIVPAGNPDIRPANPMKARYIT
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
IHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCC
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDIL
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCHHHE
ISRGNWTGFVQGIQWYANVNAQIDSPLQERNELFDSNEYNPAEPNMELVVKGDGINVRSG
EECCCCCCHHEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCC
AGLEHQTVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPSWIKINYNVPNKEVQKPKDDI
CCCCHHHHHHCCCCCEEEEEEEECCCEEECCCCEEEECCEEEEEEECCCCHHHCCCCHHC
TGGWFEGHIRKLNSLGIMYGEGNGVFAPYRNVTRAEFATLISNALKLPEGNKTFVDINKA
CCCCHHHHHHHHCCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC
HPSLHNGIKRSASAGIINGRGGGIFDPNASITREEASIMIDKALQYKGVTGELAALPFTD
CCHHHHHHHHHCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCC
QHLITYKQHVQRLYSLKVVTGVGNNEFNPKGTTTRGEAAAFLVKMLDASQK
CHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHTDQLSIAQGERGTSGLRYSDFNLETPD
CCCHHEEEEEEEEEEEEECCCCEEEHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCC
HMQINEAEVSGNEKILLEEYKHKYSESEELHKMKEAKKGQVAEEEAISNIEKAFEHVDGR
CCEEEHEECCCCCEEEHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
NDGSTREMKPVEKRRAIQYSASMTTKKYGKLAGTPFVEWIVPAGNPDIRPANPMKARYIT
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
IHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCC
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDIL
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCHHHE
ISRGNWTGFVQGIQWYANVNAQIDSPLQERNELFDSNEYNPAEPNMELVVKGDGINVRSG
EECCCCCCHHEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCC
AGLEHQTVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPSWIKINYNVPNKEVQKPKDDI
CCCCHHHHHHCCCCCEEEEEEEECCCEEECCCCEEEECCEEEEEEECCCCHHHCCCCHHC
TGGWFEGHIRKLNSLGIMYGEGNGVFAPYRNVTRAEFATLISNALKLPEGNKTFVDINKA
CCCCHHHHHHHHCCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC
HPSLHNGIKRSASAGIINGRGGGIFDPNASITREEASIMIDKALQYKGVTGELAALPFTD
CCHHHHHHHHHCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCC
QHLITYKQHVQRLYSLKVVTGVGNNEFNPKGTTTRGEAAAFLVKMLDASQK
CHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]