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The map label for this gene is vanS [H]

Identifier: 49478883

GI number: 49478883

Start: 4850659

End: 4852167

Strand: Reverse

Name: vanS [H]

Synonym: BT9727_4800

Alternate gene names: 49478883

Gene position: 4852167-4850659 (Counterclockwise)

Preceding gene: 49478884

Following gene: 49478882

Centisome position: 92.64

GC content: 31.08

Gene sequence:

>1509_bases
TTGAAAAAGATATTTAAATTATTTCATCCTTTGAATAATAAAATTAAAAGGATGTATAAAAAATTGCAATGTTATATTTT
AAAAAGTATTAGAATACAGCTCATTACAACCTTTTTTCTCTGTGCATTACTAGGGTTTGTTGTATCAAGGGCCGTAGCTC
CACTTGCTGAAGGGGCAAATTCAACAGCTTATGTTGACTACAGTACGGGTATGCAGAATATTAACTTTCAAGCAAAGCAA
ATTGCTGAAAAAGCAATAAGCGAGAATAAAATAAATAGCCTGCAAGAAATAATTAATAGTAAGGCTGAAGAGGATACAAC
TCCTTTAAAAGTATTAATTACAGATGAAACTGGAAAGGTTTTATATAAAACGAAGCAGGCACAAGAAGTAGAAATTAATT
TGCATAATACAATTAGTAATGTAATGAATTTTGCAGTAAATCAAGTTGAATTTAATAAAGATGCTTCAAATACAGAGGAA
ATACGTAAAGAGTTCCTCACCTTTTATCCAATTACGGTTGAAGGCAAAAATTTATATATGTTTGTAAGTGGGATCCCAAA
AGGATCTATTACTTATGATATAAAAGAGGGAGTTTTTCCAACACTTTTGGGAGTTATTGTATTTATTTTTTCTTTCTTTT
ATATTACTAAGAAAAAAATGAAAGAGATTGAAGTGATGGTACAAGGCGTAAATGAAATTGCAAAAGGAAACTTAGCATAT
CGAATTGAAGAGAAGGGACAAGACGAGATGGCTTTGTTAACAAAAAATATTAATCAAATGGCCGAAGGACTTATGGTGAA
TATAGAAAAAGAGAGGGAAATTGAACGACAAAAAAATGAATTGATTACAAATGTCTCGCATGATTTACGCACACCGCTTA
CTTCCATAATGGGATATTTGCGTTTATTACAAGATGCAAAGTATGAAAACAGAGAACAATATGATGAGTATATAAAAATT
GCTTTTTCAAAATCTGAACAACTCAAAAATTTAATAGAAGATTTATTTGAGTATACAAAATTAACGAATGAGAATATTGT
GTTAGAAAAACAAGAGATATGTATCAATGAAATGTTAGATCAATTAATTGAAGAATTGGTTCCACAAGCTGAAGAACATG
GTCTTTCAATTACGAAAGAGTTTCCAGAAGAACGAATACATGCTGTAGTTGATCCGGAGAAAATTGTTCGTGTATTTGAT
AATTTATTAATGAATGCAATTAAATACAGTAAGGATGAAGGAGAAATTAAAGTTTCTCTACAGCGACAAAAAGAAACCAT
TCAGATTTCTGTGAAAAATCCAAGTGAAGAATTTACAAAAAAAGAGCTGGAACATTTATTTGAACGCTTTTATAAGAAGG
ATCAGTCAAGAAGTAGAGTCTCGGAGGGGTCTGGCCTTGGACTAGCGATTGCTAAAAGCATTGTTGATATGCAAGGTGGA
GATATTCGAGCTGAATATGAAGATGGGCTGGTACAATTTATTATTACGTTACCTATTGCTACAGAATAA

Upstream 100 bases:

>100_bases
AATACCGTGATGGTACATATTCGGAAAATACGTGAAAAAATTGAAGAAAACCCGAGAAAACCTCGGTACGTTAAAACTGT
TTGGGGAGTAGGATATAAAA

Downstream 100 bases:

>100_bases
TATCATTTTTCTATTATTTTTCAAGGAGACCGGAACATATTAAGTATGTAATAGTATTACAAAAAGAACCCTTATAAGAA
GGGTTCTTTTTTTTGCAATG

Product: sensory transduction protein

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKKIFKLFHPLNNKIKRMYKKLQCYILKSIRIQLITTFFLCALLGFVVSRAVAPLAEGANSTAYVDYSTGMQNINFQAKQ
IAEKAISENKINSLQEIINSKAEEDTTPLKVLITDETGKVLYKTKQAQEVEINLHNTISNVMNFAVNQVEFNKDASNTEE
IRKEFLTFYPITVEGKNLYMFVSGIPKGSITYDIKEGVFPTLLGVIVFIFSFFYITKKKMKEIEVMVQGVNEIAKGNLAY
RIEEKGQDEMALLTKNINQMAEGLMVNIEKEREIERQKNELITNVSHDLRTPLTSIMGYLRLLQDAKYENREQYDEYIKI
AFSKSEQLKNLIEDLFEYTKLTNENIVLEKQEICINEMLDQLIEELVPQAEEHGLSITKEFPEERIHAVVDPEKIVRVFD
NLLMNAIKYSKDEGEIKVSLQRQKETIQISVKNPSEEFTKKELEHLFERFYKKDQSRSRVSEGSGLGLAIAKSIVDMQGG
DIRAEYEDGLVQFIITLPIATE

Sequences:

>Translated_502_residues
MKKIFKLFHPLNNKIKRMYKKLQCYILKSIRIQLITTFFLCALLGFVVSRAVAPLAEGANSTAYVDYSTGMQNINFQAKQ
IAEKAISENKINSLQEIINSKAEEDTTPLKVLITDETGKVLYKTKQAQEVEINLHNTISNVMNFAVNQVEFNKDASNTEE
IRKEFLTFYPITVEGKNLYMFVSGIPKGSITYDIKEGVFPTLLGVIVFIFSFFYITKKKMKEIEVMVQGVNEIAKGNLAY
RIEEKGQDEMALLTKNINQMAEGLMVNIEKEREIERQKNELITNVSHDLRTPLTSIMGYLRLLQDAKYENREQYDEYIKI
AFSKSEQLKNLIEDLFEYTKLTNENIVLEKQEICINEMLDQLIEELVPQAEEHGLSITKEFPEERIHAVVDPEKIVRVFD
NLLMNAIKYSKDEGEIKVSLQRQKETIQISVKNPSEEFTKKELEHLFERFYKKDQSRSRVSEGSGLGLAIAKSIVDMQGG
DIRAEYEDGLVQFIITLPIATE
>Mature_502_residues
MKKIFKLFHPLNNKIKRMYKKLQCYILKSIRIQLITTFFLCALLGFVVSRAVAPLAEGANSTAYVDYSTGMQNINFQAKQ
IAEKAISENKINSLQEIINSKAEEDTTPLKVLITDETGKVLYKTKQAQEVEINLHNTISNVMNFAVNQVEFNKDASNTEE
IRKEFLTFYPITVEGKNLYMFVSGIPKGSITYDIKEGVFPTLLGVIVFIFSFFYITKKKMKEIEVMVQGVNEIAKGNLAY
RIEEKGQDEMALLTKNINQMAEGLMVNIEKEREIERQKNELITNVSHDLRTPLTSIMGYLRLLQDAKYENREQYDEYIKI
AFSKSEQLKNLIEDLFEYTKLTNENIVLEKQEICINEMLDQLIEELVPQAEEHGLSITKEFPEERIHAVVDPEKIVRVFD
NLLMNAIKYSKDEGEIKVSLQRQKETIQISVKNPSEEFTKKELEHLFERFYKKDQSRSRVSEGSGLGLAIAKSIVDMQGG
DIRAEYEDGLVQFIITLPIATE

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=26.6903914590747, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=29.3040293040293, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786783, Length=297, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=28.099173553719, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789149, Length=315, Percent_Identity=26.3492063492064, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=29.0983606557377, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=29.6137339055794, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994928, Length=217, Percent_Identity=27.1889400921659, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787894, Length=346, Percent_Identity=25.1445086705202, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789808, Length=275, Percent_Identity=27.6363636363636, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI87082128, Length=307, Percent_Identity=27.6872964169381, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786600, Length=242, Percent_Identity=24.3801652892562, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788279, Length=256, Percent_Identity=25.390625, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=25.7777777777778, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=23.7668161434978, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=23.7885462555066, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787374, Length=514, Percent_Identity=21.7898832684825, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=231, Percent_Identity=22.5108225108225, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57748; Mature: 57748

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFKLFHPLNNKIKRMYKKLQCYILKSIRIQLITTFFLCALLGFVVSRAVAPLAEGAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STAYVDYSTGMQNINFQAKQIAEKAISENKINSLQEIINSKAEEDTTPLKVLITDETGKV
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCE
LYKTKQAQEVEINLHNTISNVMNFAVNQVEFNKDASNTEEIRKEFLTFYPITVEGKNLYM
EEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCEEEE
FVSGIPKGSITYDIKEGVFPTLLGVIVFIFSFFYITKKKMKEIEVMVQGVNEIAKGNLAY
EEECCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
RIEEKGQDEMALLTKNINQMAEGLMVNIEKEREIERQKNELITNVSHDLRTPLTSIMGYL
EECCCCCHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLQDAKYENREQYDEYIKIAFSKSEQLKNLIEDLFEYTKLTNENIVLEKQEICINEMLD
HHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
QLIEELVPQAEEHGLSITKEFPEERIHAVVDPEKIVRVFDNLLMNAIKYSKDEGEIKVSL
HHHHHHCCCHHHCCCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
QRQKETIQISVKNPSEEFTKKELEHLFERFYKKDQSRSRVSEGSGLGLAIAKSIVDMQGG
EECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCC
DIRAEYEDGLVQFIITLPIATE
CEEEHHHCCHHEEHHCCCCCCC
>Mature Secondary Structure
MKKIFKLFHPLNNKIKRMYKKLQCYILKSIRIQLITTFFLCALLGFVVSRAVAPLAEGAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STAYVDYSTGMQNINFQAKQIAEKAISENKINSLQEIINSKAEEDTTPLKVLITDETGKV
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCE
LYKTKQAQEVEINLHNTISNVMNFAVNQVEFNKDASNTEEIRKEFLTFYPITVEGKNLYM
EEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCEEEE
FVSGIPKGSITYDIKEGVFPTLLGVIVFIFSFFYITKKKMKEIEVMVQGVNEIAKGNLAY
EEECCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
RIEEKGQDEMALLTKNINQMAEGLMVNIEKEREIERQKNELITNVSHDLRTPLTSIMGYL
EECCCCCHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLQDAKYENREQYDEYIKIAFSKSEQLKNLIEDLFEYTKLTNENIVLEKQEICINEMLD
HHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
QLIEELVPQAEEHGLSITKEFPEERIHAVVDPEKIVRVFDNLLMNAIKYSKDEGEIKVSL
HHHHHHCCCHHHCCCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
QRQKETIQISVKNPSEEFTKKELEHLFERFYKKDQSRSRVSEGSGLGLAIAKSIVDMQGG
EECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCC
DIRAEYEDGLVQFIITLPIATE
CEEEHHHCCHHEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]