Click here to switch to the map view.

The map label for this gene is menC [H]

Identifier: 49478789

GI number: 49478789

Start: 4630822

End: 4631928

Strand: Reverse

Name: menC [H]

Synonym: BT9727_4584

Alternate gene names: 49478789

Gene position: 4631928-4630822 (Counterclockwise)

Preceding gene: 49478791

Following gene: 49481633

Centisome position: 88.43

GC content: 42.82

Gene sequence:

>1107_bases
GTGGAAATAAAAAAAGCGACACTTCATATAACGGAAATGCCACTCGTCATCCCGTTTGCTGCAAGCTACGGAACGTATGA
AAAACGTGAGAGTATCGTCATTGAATTAGAAGATGAGGACGGGTACATTGGATTTGGCGAAGTCGTTGCATTTTCTGAAC
CGTGGTATACGGAAGAAACAGTGAAGACGGCGCTGCACGTACTGCAAGACTTTTTATTGCCGGATTTATTAAAAACTGAA
ATTTCTCATCCGAATGAAGTGCCAAGTTTGTTTCAGCATATAAAAAGAAACCGAATGGCAAAGGCCGGAATTGAAGGGGC
TGTTTGGGATTTATATGCGAAGCGTCAAAAGAAATCATTAGCGACATTGCTTGGCGGAACGAAGTCTGAAATTGAAGTCG
GTGTTGTGATCGGGATCAATACGATTCCAGTTATGCTAAAACAAATCGAGAAGTACGCGGAAGAAGGATACGAGCGTTTT
AAAGTGAAAATAAAGCCAGAGCATGATTATGAGTTATTGAAAGAAATTCGTAAAGAGTTTCCGCATATCCCGTTAATGGC
TGATGCAAATTCAGCGTATACATTAGCGGATACGGAGAGTCTGAAACGACTAGATGAATTCCAATTGATGATGATTGAGC
AACCGCTAGCAGATTACGATTTTCTCGATCATGCACAGCTGCAGAAGAAGATTGAAACGCCAATTTGTCTAGACGAAAGT
ATCCATAGTTTAGAAGATGCGCGTGTTGCGATTACACTTGGTAGTTGCCAAATCGTTAACATTAAACCAGGACGAGTAGG
CGGATTAACAGAATCAATCCAAATCCATAATTATTGTATGGAGCATAACATACCAGTTTGGTGCGGCGGTATGGTAGAGA
TGGGGATTTCACGAGCGCAAAATGTTGCCCTCGCCTCATTGCCGAACTTTACGATTCCTGGCGATATATCCGCTTCTAGT
AGACATTGGGAGAAGGATATTATTTCGCCAGAAGTGACGCTTGAGGGCGGGAAAGTGATGGTGCCGCAAAGTGTTGATAC
TGAGTATAAAGTGGACCGCGGAAGGCTGGCGGAAATTACGAAGCAACGGATTGTTTTTGAGCGGTAA

Upstream 100 bases:

>100_bases
TAAAGTGCCGAAAAAAGCGTGCTTCTTAGAAGAATTACCACGAAATGCTTCGAAAAAATTGTTAAGACGAGAGTTAAGAC
AATTAGTGGAGGAGATGTAA

Downstream 100 bases:

>100_bases
GTGGAGTCTTTAAAATAGCGAGAGGGTTTTGTCGTTAATTGTCGGTAAGTCGATATATTTCAATAATCGCCGATATAATT
CGAGTTACGATCGATATATT

Product: enolase superfamily protein; N-acylamino acid racemase, mandelate racemase/muconate lactonizing enzyme, or o-succinylbenzoate synthase

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEIKKATLHITEMPLVIPFAASYGTYEKRESIVIELEDEDGYIGFGEVVAFSEPWYTEETVKTALHVLQDFLLPDLLKTE
ISHPNEVPSLFQHIKRNRMAKAGIEGAVWDLYAKRQKKSLATLLGGTKSEIEVGVVIGINTIPVMLKQIEKYAEEGYERF
KVKIKPEHDYELLKEIRKEFPHIPLMADANSAYTLADTESLKRLDEFQLMMIEQPLADYDFLDHAQLQKKIETPICLDES
IHSLEDARVAITLGSCQIVNIKPGRVGGLTESIQIHNYCMEHNIPVWCGGMVEMGISRAQNVALASLPNFTIPGDISASS
RHWEKDIISPEVTLEGGKVMVPQSVDTEYKVDRGRLAEITKQRIVFER

Sequences:

>Translated_368_residues
MEIKKATLHITEMPLVIPFAASYGTYEKRESIVIELEDEDGYIGFGEVVAFSEPWYTEETVKTALHVLQDFLLPDLLKTE
ISHPNEVPSLFQHIKRNRMAKAGIEGAVWDLYAKRQKKSLATLLGGTKSEIEVGVVIGINTIPVMLKQIEKYAEEGYERF
KVKIKPEHDYELLKEIRKEFPHIPLMADANSAYTLADTESLKRLDEFQLMMIEQPLADYDFLDHAQLQKKIETPICLDES
IHSLEDARVAITLGSCQIVNIKPGRVGGLTESIQIHNYCMEHNIPVWCGGMVEMGISRAQNVALASLPNFTIPGDISASS
RHWEKDIISPEVTLEGGKVMVPQSVDTEYKVDRGRLAEITKQRIVFER
>Mature_368_residues
MEIKKATLHITEMPLVIPFAASYGTYEKRESIVIELEDEDGYIGFGEVVAFSEPWYTEETVKTALHVLQDFLLPDLLKTE
ISHPNEVPSLFQHIKRNRMAKAGIEGAVWDLYAKRQKKSLATLLGGTKSEIEVGVVIGINTIPVMLKQIEKYAEEGYERF
KVKIKPEHDYELLKEIRKEFPHIPLMADANSAYTLADTESLKRLDEFQLMMIEQPLADYDFLDHAQLQKKIETPICLDES
IHSLEDARVAITLGSCQIVNIKPGRVGGLTESIQIHNYCMEHNIPVWCGGMVEMGISRAQNVALASLPNFTIPGDISASS
RHWEKDIISPEVTLEGGKVMVPQSVDTEYKVDRGRLAEITKQRIVFER

Specific function: Converts SHCHC to OSB [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=302, Percent_Identity=28.8079470198675, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- InterPro:   IPR010197 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.113 [H]

Molecular weight: Translated: 41611; Mature: 41611

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKKATLHITEMPLVIPFAASYGTYEKRESIVIELEDEDGYIGFGEVVAFSEPWYTEET
CCCCEEEEEEEECCEEEEECCCCCCCCCCCEEEEEEECCCCEEECCCEEEECCCCCCHHH
VKTALHVLQDFLLPDLLKTEISHPNEVPSLFQHIKRNRMAKAGIEGAVWDLYAKRQKKSL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ATLLGGTKSEIEVGVVIGINTIPVMLKQIEKYAEEGYERFKVKIKPEHDYELLKEIRKEF
HHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHC
PHIPLMADANSAYTLADTESLKRLDEFQLMMIEQPLADYDFLDHAQLQKKIETPICLDES
CCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHH
IHSLEDARVAITLGSCQIVNIKPGRVGGLTESIQIHNYCMEHNIPVWCGGMVEMGISRAQ
HHHHCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHH
NVALASLPNFTIPGDISASSRHWEKDIISPEVTLEGGKVMVPQSVDTEYKVDRGRLAEIT
CEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCEEECCCCCCCCEECCCCHHHHHH
KQRIVFER
HHHHCCCC
>Mature Secondary Structure
MEIKKATLHITEMPLVIPFAASYGTYEKRESIVIELEDEDGYIGFGEVVAFSEPWYTEET
CCCCEEEEEEEECCEEEEECCCCCCCCCCCEEEEEEECCCCEEECCCEEEECCCCCCHHH
VKTALHVLQDFLLPDLLKTEISHPNEVPSLFQHIKRNRMAKAGIEGAVWDLYAKRQKKSL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ATLLGGTKSEIEVGVVIGINTIPVMLKQIEKYAEEGYERFKVKIKPEHDYELLKEIRKEF
HHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHC
PHIPLMADANSAYTLADTESLKRLDEFQLMMIEQPLADYDFLDHAQLQKKIETPICLDES
CCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHH
IHSLEDARVAITLGSCQIVNIKPGRVGGLTESIQIHNYCMEHNIPVWCGGMVEMGISRAQ
HHHHCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHH
NVALASLPNFTIPGDISASSRHWEKDIISPEVTLEGGKVMVPQSVDTEYKVDRGRLAEIT
CEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCEEECCCCCCCCEECCCCHHHHHH
KQRIVFER
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10194342 [H]