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The map label for this gene is kinE [H]

Identifier: 49478757

GI number: 49478757

Start: 4560820

End: 4562076

Strand: Reverse

Name: kinE [H]

Synonym: BT9727_4510

Alternate gene names: 49478757

Gene position: 4562076-4560820 (Counterclockwise)

Preceding gene: 49478758

Following gene: 49478756

Centisome position: 87.1

GC content: 32.06

Gene sequence:

>1257_bases
GTGAAAGACAGTTTTCTATCATATAAAGAAGAAGAAAGGAATGCAAAAATCCTTTTGTGGGTGCTATATGTCATTGTTAT
TTCATACCAAATTTTCTATGCAATTGTATTAGAGAATAAATCACTAACGGATAAGGGACATAGCATACTATGGCAAGTTA
TTTGCGGAACTGCAATACTCGGTGTGAATGTATATTTAATAAAAAAAGAGAAAGCGAATCTTGTTAAATATGCATGTGTA
TTTGCATACTTAGGAATAGAAATTGCTAATATACTTTCGTATATGCTTTATAATGAGGCGGCATTTGATGGGGGAAATAT
AATAGAAATTATTCTCATCTTCTTTGTACCTATATTTTTAAATAAAAAATATTTTGTGTTTGTACTGTCAACTATTGTAG
TGAAATATGTGATTTATTTATTTGTATTAAAAGAAGTAAAAGGATTTATGTTCCTCATTATGTATACACTCGTATTAATA
GCAGCATACATCATTTTAATTCGATTTATACAATACCTTTCAGCGGTAAAGGAAAGTATTAAGATTGCTAGTGAATCACA
AAAATTAGCAGTAATCGGAAAAATGGCAGCGACAGTGGGACACGAAATTAAAAATCCACTTGCTTCATTAAAAGGGTTTA
CGCAATTACAACGAGAGAAGCATGGGGAGGACCCTATTTACAAACAGATGATTTTTGAAATAGAAAATATGAATAATATG
ATAAGTGAATTGATGGAAGTTGCTACATGTAAACCTTCTATTTATAAGAAACATAATATAGGTGAAATTGTATTACAAGC
AGTAGCAATTCTTCGCGAAAAAATGAATGAATGTAGTGTCCAGCTTATTTCTAATGTTGAAGAAAACATAATAGAAGTTG
AATGTGATGAGCGGAAAATGCGAGGAGTTTTTCTATATGTCATAAAAAATGCTCTGGAGGCAATGGAGCAAGGTGGCACA
TTAGAGTTACGGTTAGAAAATAAAGGACAAGATTATGTAATGCTAAGTGTAATCGATAATGGTTACGGTATTAAGAAAGA
AAATTTGGCTCGTGTTACAGAAGCCTTTTATACAACGAAACAAGATAAGATTGGTTTGGGACTTACGGTAGCAAATCGAC
TTGTTACGGAGCAGCATCTTGGTAAATTACATATTTCAAGTGAAAGAGATATTGGAACTAGAATAGATATCATTCTCCCG
AAACAACGTGGAAATAGTGAAATCCAAGTGGGAGAAAAGCTAGGAGTTACCATATGA

Upstream 100 bases:

>100_bases
TGATTTACCTCAAATGTTCGATTTCTCTTTGTTTGTCTAAGAAAATCATTTATTATAATAAATGGTATTACTTCGAGATG
TTTTTTGAAGGGGTGTTGAA

Downstream 100 bases:

>100_bases
ATAAAGACGATATATTTAAAAATGAAGAAATGAAGGCGCTAAAAATATTTTTAAGTTTATTTTTCATCATATTTTTTGTA
TACGATCTTGCCTACGAATT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKDSFLSYKEEERNAKILLWVLYVIVISYQIFYAIVLENKSLTDKGHSILWQVICGTAILGVNVYLIKKEKANLVKYACV
FAYLGIEIANILSYMLYNEAAFDGGNIIEIILIFFVPIFLNKKYFVFVLSTIVVKYVIYLFVLKEVKGFMFLIMYTLVLI
AAYIILIRFIQYLSAVKESIKIASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEENIIEVECDERKMRGVFLYVIKNALEAMEQGGT
LELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTKQDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILP
KQRGNSEIQVGEKLGVTI

Sequences:

>Translated_418_residues
MKDSFLSYKEEERNAKILLWVLYVIVISYQIFYAIVLENKSLTDKGHSILWQVICGTAILGVNVYLIKKEKANLVKYACV
FAYLGIEIANILSYMLYNEAAFDGGNIIEIILIFFVPIFLNKKYFVFVLSTIVVKYVIYLFVLKEVKGFMFLIMYTLVLI
AAYIILIRFIQYLSAVKESIKIASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEENIIEVECDERKMRGVFLYVIKNALEAMEQGGT
LELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTKQDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILP
KQRGNSEIQVGEKLGVTI
>Mature_418_residues
MKDSFLSYKEEERNAKILLWVLYVIVISYQIFYAIVLENKSLTDKGHSILWQVICGTAILGVNVYLIKKEKANLVKYACV
FAYLGIEIANILSYMLYNEAAFDGGNIIEIILIFFVPIFLNKKYFVFVLSTIVVKYVIYLFVLKEVKGFMFLIMYTLVLI
AAYIILIRFIQYLSAVKESIKIASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEENIIEVECDERKMRGVFLYVIKNALEAMEQGGT
LELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTKQDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILP
KQRGNSEIQVGEKLGVTI

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=25.7261410788382, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1790436, Length=256, Percent_Identity=26.953125, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790300, Length=203, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=23.5555555555556, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47576; Mature: 47576

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50109 HIS_KIN ; PS00838 INTERLEUKIN_4_13

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSFLSYKEEERNAKILLWVLYVIVISYQIFYAIVLENKSLTDKGHSILWQVICGTAIL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHH
GVNVYLIKKEKANLVKYACVFAYLGIEIANILSYMLYNEAAFDGGNIIEIILIFFVPIFL
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NKKYFVFVLSTIVVKYVIYLFVLKEVKGFMFLIMYTLVLIAAYIILIRFIQYLSAVKESI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEENIIEVECDERKM
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHH
RGVFLYVIKNALEAMEQGGTLELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCC
QDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILPKQRGNSEIQVGEKLGVTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MKDSFLSYKEEERNAKILLWVLYVIVISYQIFYAIVLENKSLTDKGHSILWQVICGTAIL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHH
GVNVYLIKKEKANLVKYACVFAYLGIEIANILSYMLYNEAAFDGGNIIEIILIFFVPIFL
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NKKYFVFVLSTIVVKYVIYLFVLKEVKGFMFLIMYTLVLIAAYIILIRFIQYLSAVKESI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEENIIEVECDERKM
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHH
RGVFLYVIKNALEAMEQGGTLELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCC
QDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILPKQRGNSEIQVGEKLGVTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]