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The map label for this gene is metK

Identifier: 49478753

GI number: 49478753

Start: 4551814

End: 4553013

Strand: Reverse

Name: metK

Synonym: BT9727_4500

Alternate gene names: 49478753

Gene position: 4553013-4551814 (Counterclockwise)

Preceding gene: 49481328

Following gene: 49478751

Centisome position: 86.93

GC content: 41.5

Gene sequence:

>1200_bases
ATGACAAAAAAACGTCATCTGTTCACATCTGAGTCTGTAACTGAAGGACATCCAGATAAAATTTGTGACCAAATTTCTGA
TTCAATTTTAGATGCGATCTTATCAAAAGACGCAAATGCACGTGTAGCTTGTGAAACAACTGTAACAACTGGTTTAGTAT
TGGTAGCGGGGGAAATTACGACTTCTACTTACGTAGATATTCCAAAAATCGTTCGTGAAACAATTCAAGGCATTGGTTAC
ACACGCGCAAAATACGGATTCGATGCAGAAACTTGTGCAGTTTTAACATCTATCGATGAGCAATCTGCTGACATCGCTAT
GGGTGTTGACCAAGCGCTAGAAGCACGCGAAGGTCAAATGACTGACGCTGAGATTGAGGCAATTGGTGCAGGAGACCAAG
GTTTAATGTTTGGTTTCGCATGTAATGAAACACAAGAGTTAATGCCACTTCCAATCTCGCTTGCTCACAAATTAGCTCGC
CGTTTAACTGAAGTACGTAAAAATGATACATTATCATACCTACGTCCGGATGGAAAAACGCAAGTTACAGTTGAGTATGA
TGAAAATGGTAAACCAGTACGTGTTGATACTATCGTAATTTCTACACAACATCACCCAGATGTTACATGGGAAGAAATCG
ATCGCGACTTAAAAGAGCACGTAATTAAAGCTGTAGTACCAGCAGAATTAATCGATGGAGAAACGAAATTCTTCATTAAC
CCAACTGGCCGCTTCGTAATTGGTGGACCACAAGGTGATGCTGGTTTAACAGGACGTAAAATCATCGTTGATACTTACGG
TGGATACGCTCGCCATGGTGGCGGTGCATTCTCTGGTAAAGATGCAACGAAAGTTGACCGTTCTGCAGCATATGCAGCTC
GTTATGTTGCGAAAAACATCGTAGCAGCTGGTCTTGCTGAAAAAGCAGAAGTACAACTTGCATACGCAATTGGTGTAGCA
CAACCAGTATCAATTTCAGTTGATACATTCGGCACTGGTAAAGTATCTGAAGACGTATTAGTAGAACTAGTTCGTAACAA
CTTCGATCTTCGCCCAGCTGGTATTATTAAAATGCTAGACTTACGTCGCCCAATTTACAAACAAACAGCAGCTTACGGCC
ACTTCGGACGTACTGATGTAGATCTAACATGGGAACGTACAGACAAAGCTGCAGCTTTAAAAGAGCAAGCTGGTCTATAA

Upstream 100 bases:

>100_bases
CTGTATGCTTGTGTGGGAGAATAAATGTATGTCGCAATCTGTGGCAAATTAAGGATGAGTTCCGTACAATATATACAATT
ACTGTAGGGAGGTTTACCAC

Downstream 100 bases:

>100_bases
TATATGAAAAAAAGCTTTGCGCAGTGAGCGCAAAGCTTTTTTTATATATAATTACGTTGGAATAAAGCGAAGAATTCAAT
ATCATGCCAAGTGCCATTGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLAR
RLTEVRKNDTLSYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVA
QPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLTWERTDKAAALKEQAGL

Sequences:

>Translated_399_residues
MTKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLAR
RLTEVRKNDTLSYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVA
QPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLTWERTDKAAALKEQAGL
>Mature_398_residues
TKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGYT
RAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLARR
LTEVRKNDTLSYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQ
PVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLTWERTDKAAALKEQAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=58.9005235602094, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=59.4240837696335, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=63.0379746835443, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=55.5269922879177, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.1020408163265, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=54.7557840616967, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=53.1328320802005, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=50.3856041131105, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=54.1420118343195, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=53.3724340175953, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=54.3408360128617, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=52.8735632183908, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=65.9090909090909, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=55.8746736292428, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=54.70737913486, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=382, Percent_Identity=56.8062827225131, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580529, Length=382, Percent_Identity=56.8062827225131, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580537, Length=382, Percent_Identity=56.8062827225131, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580533, Length=382, Percent_Identity=56.8062827225131, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580535, Length=382, Percent_Identity=56.8062827225131, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580531, Length=382, Percent_Identity=56.8062827225131, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=55.7591623036649, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=55.7591623036649, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=54.5945945945946, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=54.3307086614173, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43277; Mature: 43146

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEIT
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHCCEEEEEECCC
TSTYVDIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQM
CCHHCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
TDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLARRLTEVRKNDTLSYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCE
QVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
EEEEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLD
HHHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLTWERTDKAAALKEQAGL
HCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
TKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEIT
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHCCEEEEEECCC
TSTYVDIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQM
CCHHCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
TDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLARRLTEVRKNDTLSYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCE
QVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
EEEEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLD
HHHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLTWERTDKAAALKEQAGL
HCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA