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The map label for this gene is amyX

Identifier: 49478732

GI number: 49478732

Start: 4492058

End: 4494199

Strand: Reverse

Name: amyX

Synonym: BT9727_4434

Alternate gene names: 49478732

Gene position: 4494199-4492058 (Counterclockwise)

Preceding gene: 49481476

Following gene: 49478731

Centisome position: 85.81

GC content: 37.39

Gene sequence:

>2142_bases
ATGTTGAAAGTAAAACGTCCATTTGATGCCTATTTAGATGAAATGAATAAAATTACAATTTTGCTCCCACATGCGTATGG
AACAAGTCGGACATTTCGTTTGCAAGAAGGAAGTAACGTGAAAGATTTACCGATTGCTCATACAATTGCGCTTCCGGATG
CAACGAAGTATGAGTGCTTTATAGAAGAGCCGCTAGATGTGGGGAAGTATTATACAGTACGAGATGAACGGAATGAAGAA
ACAGATTTACAAATAGGTGCTGTCATAAGAACAGAGATTTTCGATGAAAAGTATTATTACGAAGGTACAGATTTAGGGGC
TGTATATCAAGAAGAAGCAACTACATTTAAAGTATGGGCTCCAACTGCGAGGCTTGCGAAAGTAAGAATTTATAAAAGTG
ATAAAGAATATACCGATCACGAAATGCACAGAGAAGAAAATGGGGTATGGGCTCATACATTACAAGGCGATTTAGCCGGG
GAAAAGTATACATTTCTTGTTTGTATTAATTTAATATGGAATGAAGCTGTGGATCCTTATGCAAAATCAGTGACTGTAAA
TGGGAAGTACGGCGTTGTTATCGATTTGGAAAAAACAAATGTAACAAAGCAAGAGCAATTACCACCATTACAAGCAATGA
CAGATGCTATATTGTATGAACTGCATATTCGTGATGCTACTATTCATGAGGGCAGTGGAGTGAGTAAAAAAGGAACATAT
AAAGGGCTAATGGAAGAAGGAACAAAAGGACAAAATGGGACGTTAACAGGTTTGTCTCATATAAAAGATTTAGGTGTTAC
ACATGTTGAATTATTGCCTTTATATTGTTTCGGTGGTGTAGATGAGGCGAATCCTTCTTCTGCATATAATTGGGGTTACA
ATCCATTATATTACAACGCACCAACGGGGTTTTATGCTACAAACCCATCTGACCCGTATAACAGGATCGTAGAGTGTAAA
GAGCTGATTGAAACATTTCATGAACACGGTATTAGGGTGATTATAGATGTTGTATATAATCATGTGTATGAAAGAGAGCT
ATCATCATTTGAAAAGCTTGTCCCAGGATATTATTTTCGTCATGGTGAAAATGGTATGCCTTCTAATGGGACGGGAGTCG
GAAATGATATAGCATCTGAACGGAAAATGATGAGGAAATTCATTGTAGAGTCTATTTTATATTGGCTTACGGAATACAAT
GTTGATGGATTCCGGTTCGACTTAATGGGGATTTTAGATGTCGATACAATCAATATAATAGAAAAAGAAGTGCGAAACAT
AAAGCGAGATGCATTGCTATTAGGAGAAGGGTGGGATCTGCAAACACCACTTCCTCTAGAGGAAAAAGCAACGTTAAATA
ATGCAAACAAAATGCCGCATATTGCGCAATTTAATGATCAATTTCGTGATGGGATAAAGGGAAGTACCTTTCATATAAAT
AAACGTGGTTTTGCATTTGGTGGGCATGTAGACCGTAATCATTTGCAGTATATAGTATCAGGAAGCCTTTTGAGTATGAA
AGAAACAGGTTTGTTTCTAGAGCCTGTGCAAAGCATTAACTATGTAGAATGTCATGACAATATGACGATGTGGGATAAAC
TAATGCGCAGTAATGAAGAGTCGGAAGAGATTTTGAAAAAACGTCACGTGCTAGCAACTGCAATGGCGATTCTGTCGCAA
GGCATCCCTTTCTTACATGCTGGGCAAGAGTTTTATCGTACGAAGCAAGGGAATGAAAATAGTTATAATGCAAATGATGA
AATTAATCAATTAGATTGGGATCGAAAAGAGAAAGAAATAGAGACCGTTAACTATATAAAAGGTTTAATTGCGATTCGTA
AAGAGCACGGAGCATTCCGATTACAAAACGCAGACTTAATAAAAAAGCACATGACTTTTTTGCAAACATCGCCAGAAGTA
CTTGCATACCACTTAGAGCACGTAGAATCGTTTGGACCGTGGAAAGAGATTGTCGTTTTATTTAATAGCAGTTTAGAAGC
GAAAACGGTGCAACTTCCGAAAGAAGAAACGTGGCATGTGTTAGTAAACGAAAAGCAAGCGAAAATAGAGCCAATTTCTT
CATTTAGAGGAAAGGAACTTAGGCTCTCTCCAATTAGCACGTATATATTGACGATAATGTGA

Upstream 100 bases:

>100_bases
ATAAAGAATAATGTGTTACTGTATAGGGCTTCGCTGATAAGAAAAGCCTTATCATTTGCAGCTCGTTTCATTTTAACTTT
AAGAATGATGGTGATTTTTT

Downstream 100 bases:

>100_bases
CAAATCGCTACTTGACGGTGAGAATGTAATAAATGTAGAATTGACAAAGGTGACGATTTTGATAAGTTGTCATTCTATGG
CTGGTTTTGTAAAATAAAAT

Product: pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE
TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQEEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG
EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
KGLMEEGTKGQNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRIVECK
ELIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN
VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESEEILKKRHVLATAMAILSQ
GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV
LAYHLEHVESFGPWKEIVVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM

Sequences:

>Translated_713_residues
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE
TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQEEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG
EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
KGLMEEGTKGQNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRIVECK
ELIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN
VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESEEILKKRHVLATAMAILSQ
GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV
LAYHLEHVESFGPWKEIVVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
>Mature_713_residues
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE
TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQEEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG
EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
KGLMEEGTKGQNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRIVECK
ELIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN
VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESEEILKKRHVLATAMAILSQ
GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV
LAYHLEHVESFGPWKEIVVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=669, Percent_Identity=23.0194319880419, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 81692; Mature: 81692

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECF
CCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEE
IEEPLDVGKYYTVRDERNEETDLQIGAVIRTEIFDEKYYYEGTDLGAVYQEEATTFKVWA
ECCCCCCCCEEEECCCCCCCCCEEEHHEEEHHHHCCCEEECCCCCCCEECCCCCEEEEEC
PTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAGEKYTFLVCINLIWNEAVDPY
CCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCH
AKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
HHEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHEEEEEECCEEEECCCCCCCCCCH
KGLMEEGTKGQNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNA
HHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHEECCCCCCCCCCCCCCCCCCEEECC
PTGFYATNPSDPYNRIVECKELIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFR
CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
HGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYNVDGFRFDLMGILDVDTINII
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHH
EKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
HHHHHHHHHCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHCHHHHCCCCCCEEEEE
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEE
CCCEEECCCCCCCHHEEEECCCEEEHHHCCCCHHHHCCCCEEEECCCHHHHHHHHHCCCH
SEEILKKRHVLATAMAILSQGIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEI
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
ETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEVLAYHLEHVESFGPWKEIVVL
HHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHEE
FNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
ECCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEECHHCEEEEEEC
>Mature Secondary Structure
MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECF
CCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEE
IEEPLDVGKYYTVRDERNEETDLQIGAVIRTEIFDEKYYYEGTDLGAVYQEEATTFKVWA
ECCCCCCCCEEEECCCCCCCCCEEEHHEEEHHHHCCCEEECCCCCCCEECCCCCEEEEEC
PTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAGEKYTFLVCINLIWNEAVDPY
CCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCH
AKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY
HHEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHEEEEEECCEEEECCCCCCCCCCH
KGLMEEGTKGQNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNA
HHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHEECCCCCCCCCCCCCCCCCCEEECC
PTGFYATNPSDPYNRIVECKELIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFR
CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
HGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYNVDGFRFDLMGILDVDTINII
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHH
EKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN
HHHHHHHHHCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHCHHHHCCCCCCEEEEE
KRGFAFGGHVDRNHLQYIVSGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEE
CCCEEECCCCCCCHHEEEECCCEEEHHHCCCCHHHHCCCCEEEECCCHHHHHHHHHCCCH
SEEILKKRHVLATAMAILSQGIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEI
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
ETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEVLAYHLEHVESFGPWKEIVVL
HHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHEE
FNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
ECCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEECHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]