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The map label for this gene is walK [H]

Identifier: 49478723

GI number: 49478723

Start: 4463745

End: 4465199

Strand: Reverse

Name: walK [H]

Synonym: BT9727_4401

Alternate gene names: 49478723

Gene position: 4465199-4463745 (Counterclockwise)

Preceding gene: 49480706

Following gene: 49478722

Centisome position: 85.25

GC content: 33.26

Gene sequence:

>1455_bases
ATGTCTATTAAAACAAGGTTTTTATTTTCTTATATCGCTGTCATTCTCGTTTCCATTACGCTTATATTAATCGCGGGATT
TTTAATCGTTTTTTCGATAACAGGTGATTTGGAAGCAGTGAAAAATTTCTATAAAAGCTCCTATATTCAAAAGCCGCTTA
CGTCAGAAGAAGAGAATGTGTATATTGAGTTAAAGACGGCAGCAAAGAAACATCAATCGCAACTATTAGATGAATCATTC
GTTTCATCACTTGAAAAAGAAGGGGTGAAAATTGTTGTAAGAAAGGGAGAAACCATTTCTTATGCTACAAAAGTACTTGA
AAGTGGGGCTTTAAAGGAAGCTCTTCCGAAATTTGAATCAGCAAATATTAATAGTCGTGGTACAACGGAATTAGGCGATA
CATTTTATCGATATGTAAAGTTTGATTTTTATTTTCCTCAGGAAGAAGAGGGGAGTATATTTGTATTAAAGAAGCAAAGT
TCATTTGTAGATCTTACACAAAAGTTATTTCCGATTTTGTTCGTATCACTTTTACTGTTAGCTATTTTACTTATTGGATT
ATTAAGTTACCTCGTCTCAAGAAGTGTTATAAAACCAATCTTTGTATTGAAAGATGCGACTGAAAAAATTAAAGAAGGAA
ATTTAGATTTTCAAATACCAGTTACATCGCACGATGAAATTGGGCAATTGAATCAAGGATTTGAGGAAATGAGGAAGAAA
TTAAAAGAATCGATAGAGATGCAAACGCAGTATGAAGAAAATCGAAAAGAGCTCATTTCAAACATCTCTCACGATTTAAA
AACACCGATTACATCTATTATTGGATATGTAGAAGGAATAAAAGACGGAGTAGCAAATACGCCAGAAAAAATGGATAAGT
ATTTGACAACTATCCATACGAAAGCAAGACATATGGATACACTCATTGATGAATTATTTTTATTCTCAAAGCTCGATTTG
AATCGAGTTCCCTTTCAGTTTGAAACGGTTGAATTAAATATGTTTATGCAAGAATTAATAGAAGAGATGCAGATGGATTT
AAGTAAAGAAGGTATAGAAGTTAACTTACAATTACATGCATCACCACTTTATGTAAAGGCTGATTGCGAAAAGATAAATA
GAGTGATATCAAATTTAATTCATAATAGTGTGAAATACATGGATAAAGAAGAAAAGAAAATTACTGTAACAGTATCGAGT
GATAACAATAAAGTGATTGTGAAAGTAATGGACAACGGATCAGGTATAGAATCTGATACACTTCCTTATATTTTTGAACG
TTTCTATCGTGCAGAGCAATCGAGAAATTCTAGTACAGGTGGAAGTGGACTTGGCTTAGCGATAGCGAAGCAAATTATTG
AAGAACATGGCGGGGAAATTTGGGCGGAAAGCGAGCTTGGGGAAGGCACAAGCATTTTCTTCTCATTGGAAAAAGTAGAG
AAATGTGGTGAGTAA

Upstream 100 bases:

>100_bases
TATAAACTGACGCTAAAATTACTATAAAATCGCTTGAGTATTTGAACATATATTTTTATGCTTATGAGTAATAAGGTTTG
ATAAAGTGAGGATGTAACAT

Downstream 100 bases:

>100_bases
ATTGAAAAGAATTTTACTAATAGAAGATGAAGTAAGCATTGCAGAATTACAGCGAGATTATTTAGAAATTAATGATTTTC
AAGTTGATGTCGAACATTCA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSIKTRFLFSYIAVILVSITLILIAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENVYIELKTAAKKHQSQLLDESF
VSSLEKEGVKIVVRKGETISYATKVLESGALKEALPKFESANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQS
SFVDLTQKLFPILFVSLLLLAILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHTKARHMDTLIDELFLFSKLDL
NRVPFQFETVELNMFMQELIEEMQMDLSKEGIEVNLQLHASPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSS
DNNKVIVKVMDNGSGIESDTLPYIFERFYRAEQSRNSSTGGSGLGLAIAKQIIEEHGGEIWAESELGEGTSIFFSLEKVE
KCGE

Sequences:

>Translated_484_residues
MSIKTRFLFSYIAVILVSITLILIAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENVYIELKTAAKKHQSQLLDESF
VSSLEKEGVKIVVRKGETISYATKVLESGALKEALPKFESANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQS
SFVDLTQKLFPILFVSLLLLAILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHTKARHMDTLIDELFLFSKLDL
NRVPFQFETVELNMFMQELIEEMQMDLSKEGIEVNLQLHASPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSS
DNNKVIVKVMDNGSGIESDTLPYIFERFYRAEQSRNSSTGGSGLGLAIAKQIIEEHGGEIWAESELGEGTSIFFSLEKVE
KCGE
>Mature_483_residues
SIKTRFLFSYIAVILVSITLILIAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENVYIELKTAAKKHQSQLLDESFV
SSLEKEGVKIVVRKGETISYATKVLESGALKEALPKFESANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQSS
FVDLTQKLFPILFVSLLLLAILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKKL
KESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHTKARHMDTLIDELFLFSKLDLN
RVPFQFETVELNMFMQELIEEMQMDLSKEGIEVNLQLHASPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSSD
NNKVIVKVMDNGSGIESDTLPYIFERFYRAEQSRNSSTGGSGLGLAIAKQIIEEHGGEIWAESELGEGTSIFFSLEKVEK
CGE

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=280, Percent_Identity=30.7142857142857, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=31.1111111111111, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1790346, Length=271, Percent_Identity=25.4612546125461, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789149, Length=342, Percent_Identity=24.5614035087719, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=26.8595041322314, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790861, Length=295, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787894, Length=344, Percent_Identity=23.8372093023256, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081816, Length=337, Percent_Identity=24.6290801186944, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786783, Length=314, Percent_Identity=25.4777070063694, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082128, Length=311, Percent_Identity=25.08038585209, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789808, Length=300, Percent_Identity=24.6666666666667, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=23.8683127572016, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=26.2443438914027, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=26.2931034482759, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=25.3424657534247, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=24.3589743589744, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788549, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789403, Length=202, Percent_Identity=27.2277227722772, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54893; Mature: 54762

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKTRFLFSYIAVILVSITLILIAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCE
YIELKTAAKKHQSQLLDESFVSSLEKEGVKIVVRKGETISYATKVLESGALKEALPKFES
EEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHCCCCC
ANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQSSFVDLTQKLFPILFVSLLLL
CCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KARHMDTLIDELFLFSKLDLNRVPFQFETVELNMFMQELIEEMQMDLSKEGIEVNLQLHA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
SPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSSDNNKVIVKVMDNGSGIESDT
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCC
LPYIFERFYRAEQSRNSSTGGSGLGLAIAKQIIEEHGGEIWAESELGEGTSIFFSLEKVE
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEHHHHH
KCGE
HCCC
>Mature Secondary Structure 
SIKTRFLFSYIAVILVSITLILIAGFLIVFSITGDLEAVKNFYKSSYIQKPLTSEEENV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCE
YIELKTAAKKHQSQLLDESFVSSLEKEGVKIVVRKGETISYATKVLESGALKEALPKFES
EEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHCCCCC
ANINSRGTTELGDTFYRYVKFDFYFPQEEEGSIFVLKKQSSFVDLTQKLFPILFVSLLLL
CCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AILLIGLLSYLVSRSVIKPIFVLKDATEKIKEGNLDFQIPVTSHDEIGQLNQGFEEMRKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
LKESIEMQTQYEENRKELISNISHDLKTPITSIIGYVEGIKDGVANTPEKMDKYLTTIHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KARHMDTLIDELFLFSKLDLNRVPFQFETVELNMFMQELIEEMQMDLSKEGIEVNLQLHA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
SPLYVKADCEKINRVISNLIHNSVKYMDKEEKKITVTVSSDNNKVIVKVMDNGSGIESDT
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCC
LPYIFERFYRAEQSRNSSTGGSGLGLAIAKQIIEEHGGEIWAESELGEGTSIFFSLEKVE
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEHHHHH
KCGE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA