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The map label for this gene is rluF [H]

Identifier: 49478685

GI number: 49478685

Start: 4349036

End: 4349725

Strand: Reverse

Name: rluF [H]

Synonym: BT9727_4284

Alternate gene names: 49478685

Gene position: 4349725-4349036 (Counterclockwise)

Preceding gene: 49478686

Following gene: 49478816

Centisome position: 83.05

GC content: 38.12

Gene sequence:

>690_bases
ATGAAAATCAACCAATACATAAGCGAAGCAAAATCCTGTTCAAGACGTGAAACAGATCGCCTTATTAAAGCCGGGCGCGT
AGCGATTAACGGCGAAATATGTACGCACGGTGCACTTGTTAATGAAGGTGATGTTGTAACGATTGATGGACAGGTTATTG
CAAAAGAAGAGAAAGAAAAGGTGTATATTGCCTTTCATAAACCAGTTGGGATTACCTGCACAGCAGCTGAGCATATTGAA
GGTAATATTATTGATTATATTAATTACCCAGAGCGTATCTTTCCTGTTGGACGATTAGATAAAGCATCAGAAGGACTTAT
TTTATTAACGAATGACGGCGGGATTGCCAATCAAATTTTACACGGTGATCATGAGCACGAGAAAGAATATGTTGTTACAG
TTGATAAACCTTTTACAGATTGGTTTATGAATGAGATGTCCAGCGGTGTAAAGATTAAAGATGGAATGACGAAGCCATGT
AAAGTGACGAGAGTGGATCAGTCTACGTTTCGCATCGTTTTAACGCAAGGAATGAACAGACAAATTCGTAGAATGAGCCG
AGCTTTCGGGTTTACTGTAATAAAGTTAAAGCGAGAACGCATTATGAATATAGAGCTAAATGATCTTGAAGTAGGAAAGT
GGCGAGACATTACAACTGAAGAATTAGAATTATTAATAGGACAGTTATAG

Upstream 100 bases:

>100_bases
TATTTATTTAGTTGTTTTTGCAAAGTAAACGAGCGTCAATCGTGGCAGAGTAATATTGCCATAGATTGACGCTTTTAATA
TATATGAAAAGGAATAAGTT

Downstream 100 bases:

>100_bases
GGAGGGAAAATTTTCTTCCTATATTTTTTTGTCAGAAAAAATAAAACTATTGATTTTTTAAAAAATAGCCCATATAATAA
AAAACAACAAGTTTCGACAA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKINQYISEAKSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEKVYIAFHKPVGITCTAAEHIE
GNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQILHGDHEHEKEYVVTVDKPFTDWFMNEMSSGVKIKDGMTKPC
KVTRVDQSTFRIVLTQGMNRQIRRMSRAFGFTVIKLKRERIMNIELNDLEVGKWRDITTEELELLIGQL

Sequences:

>Translated_229_residues
MKINQYISEAKSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEKVYIAFHKPVGITCTAAEHIE
GNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQILHGDHEHEKEYVVTVDKPFTDWFMNEMSSGVKIKDGMTKPC
KVTRVDQSTFRIVLTQGMNRQIRRMSRAFGFTVIKLKRERIMNIELNDLEVGKWRDITTEELELLIGQL
>Mature_229_residues
MKINQYISEAKSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEKVYIAFHKPVGITCTAAEHIE
GNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQILHGDHEHEKEYVVTVDKPFTDWFMNEMSSGVKIKDGMTKPC
KVTRVDQSTFRIVLTQGMNRQIRRMSRAFGFTVIKLKRERIMNIELNDLEVGKWRDITTEELELLIGQL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=223, Percent_Identity=51.5695067264574, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=30.4721030042918, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=32.7800829875519, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=27.4725274725275, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 25991; Mature: 25991

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINQYISEAKSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEK
CCHHHHHHHHHHHHHHHHHHHHHHCEEEECCEEEECCEEECCCCEEEECCEEEEECCCCE
VYIAFHKPVGITCTAAEHIEGNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQIL
EEEEEECCCCEEEEEHHCCCCCEEEECCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHH
HGDHEHEKEYVVTVDKPFTDWFMNEMSSGVKIKDGMTKPCKVTRVDQSTFRIVLTQGMNR
CCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCH
QIRRMSRAFGFTVIKLKRERIMNIELNDLEVGKWRDITTEELELLIGQL
HHHHHHHHHCEEEEEEEHHHEEEEEECCEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKINQYISEAKSCSRRETDRLIKAGRVAINGEICTHGALVNEGDVVTIDGQVIAKEEKEK
CCHHHHHHHHHHHHHHHHHHHHHHCEEEECCEEEECCEEECCCCEEEECCEEEEECCCCE
VYIAFHKPVGITCTAAEHIEGNIIDYINYPERIFPVGRLDKASEGLILLTNDGGIANQIL
EEEEEECCCCEEEEEHHCCCCCEEEECCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHH
HGDHEHEKEYVVTVDKPFTDWFMNEMSSGVKIKDGMTKPCKVTRVDQSTFRIVLTQGMNR
CCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCH
QIRRMSRAFGFTVIKLKRERIMNIELNDLEVGKWRDITTEELELLIGQL
HHHHHHHHHCEEEEEEEHHHEEEEEECCEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]