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The map label for this gene is murG

Identifier: 49478592

GI number: 49478592

Start: 4055550

End: 4056608

Strand: Reverse

Name: murG

Synonym: BT9727_3994

Alternate gene names: 49478592

Gene position: 4056608-4055550 (Counterclockwise)

Preceding gene: 49478593

Following gene: 49480649

Centisome position: 77.45

GC content: 35.69

Gene sequence:

>1059_bases
ATGAAAAAAATAGTCTTTACAGGTGGCGGTTCGGCAGGGCATGTAACACCTAATTTAGCCATTATTCCATATTTAAAGGA
AGACAACTGGGACATTTCTTATATTGGTTCTCATCAAGGAATTGAGAAGACGATTATAGAAAAGGAAGATATTCCGTACT
ATAGTATTGCGAGTGGAAAGTTACGCCGTTATTTTGATTTGAAAAATATAAAAGATCCTTTTCTTGTGATGAAGGGTGTT
ATGGACGCGTATGTAAGGATTCGAAAACTAAAACCAGATGTGATTTTTTCAAAAGGTGGTTTCGTATCTGTACCAGTCGT
AATTGGAGGATGGCTAAATAGAGTACCAGTTTTATTACATGAGTCTGATATGACGCCAGGACTAGCAAACAAAATTGCGC
TCCGTTTCGCTTCAAAAATATTTGTTACATTTGAAGAAGCTGCGAAACATTTACCGAAAGAAAAAGTAATTTATACAGGG
TCTCCTGTACGTGAAGAAGTACTAAAGGGAAATCGTGAAAAAGCTTTAGCGTTTTTAGGTTTTTCACGTAAAAAGCCAGT
TATTACAATTATGGGAGGAAGTTTGGGCGCAAAAAAAATTAACGAAACGGTTCGAGAAGCGTTACCAGAACTTCTGAGAA
AATATCAAATTGTTCACCTTTGTGGAAAAGGTAATCTTGATGATAGTTTACAAAATAAAGAAGGATATAGACAATTTGAA
TATGTACACGGGGAGCTGCCGGATATATTAGCGATAACAGATTTTGTTATTTCACGTGCGGGGTCCAACGCTATTTTTGA
GTTTTTAACATTGCAAAAGCCGATGCTTCTAATTCCGCTATCGAAATTTGCAAGTCGTGGAGACCAAATATTAAATGCAG
AATCATTTGAAAGGCAAGGATATGCCTCTGTACTGTATGAAGAAGATGTGACTGTCAATTCTCTTATAAAGCATGTTGAA
GAGTTATCTCATAATAATGAGGCATATAAAACCGCATTAAAAAAATATAATGGAAAAGAAGCGATTCAAACAATCATTCA
CCATATTTCAGAGGCATGA

Upstream 100 bases:

>100_bases
AGGGTTTATGATGGAACCAGAAGCATCTGTAACGGCTATGGTATTTTCTCACCCCGAGGCAAGATATTTTAGTGTATTAT
AGTCTAGAAGGGGGAGACGT

Downstream 100 bases:

>100_bases
TGAAACAATCATGCCTCTTTTAGTAGGATGAAAGTTTCCAAGAAACGGTGTTTTTATGTTACAATAGCAAATGGTTAGTA
GGGAAATTCCAATTAATGAA

Product: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

Products: NA

Alternate protein names: Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase 2 [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKKIVFTGGGSAGHVTPNLAIIPYLKEDNWDISYIGSHQGIEKTIIEKEDIPYYSIASGKLRRYFDLKNIKDPFLVMKGV
MDAYVRIRKLKPDVIFSKGGFVSVPVVIGGWLNRVPVLLHESDMTPGLANKIALRFASKIFVTFEEAAKHLPKEKVIYTG
SPVREEVLKGNREKALAFLGFSRKKPVITIMGGSLGAKKINETVREALPELLRKYQIVHLCGKGNLDDSLQNKEGYRQFE
YVHGELPDILAITDFVISRAGSNAIFEFLTLQKPMLLIPLSKFASRGDQILNAESFERQGYASVLYEEDVTVNSLIKHVE
ELSHNNEAYKTALKKYNGKEAIQTIIHHISEA

Sequences:

>Translated_352_residues
MKKIVFTGGGSAGHVTPNLAIIPYLKEDNWDISYIGSHQGIEKTIIEKEDIPYYSIASGKLRRYFDLKNIKDPFLVMKGV
MDAYVRIRKLKPDVIFSKGGFVSVPVVIGGWLNRVPVLLHESDMTPGLANKIALRFASKIFVTFEEAAKHLPKEKVIYTG
SPVREEVLKGNREKALAFLGFSRKKPVITIMGGSLGAKKINETVREALPELLRKYQIVHLCGKGNLDDSLQNKEGYRQFE
YVHGELPDILAITDFVISRAGSNAIFEFLTLQKPMLLIPLSKFASRGDQILNAESFERQGYASVLYEEDVTVNSLIKHVE
ELSHNNEAYKTALKKYNGKEAIQTIIHHISEA
>Mature_352_residues
MKKIVFTGGGSAGHVTPNLAIIPYLKEDNWDISYIGSHQGIEKTIIEKEDIPYYSIASGKLRRYFDLKNIKDPFLVMKGV
MDAYVRIRKLKPDVIFSKGGFVSVPVVIGGWLNRVPVLLHESDMTPGLANKIALRFASKIFVTFEEAAKHLPKEKVIYTG
SPVREEVLKGNREKALAFLGFSRKKPVITIMGGSLGAKKINETVREALPELLRKYQIVHLCGKGNLDDSLQNKEGYRQFE
YVHGELPDILAITDFVISRAGSNAIFEFLTLQKPMLLIPLSKFASRGDQILNAESFERQGYASVLYEEDVTVNSLIKHVE
ELSHNNEAYKTALKKYNGKEAIQTIIHHISEA

Specific function: Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II) [H]

COG id: COG0707

COG function: function code M; UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 28 family. MurG subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786278, Length=320, Percent_Identity=28.4375, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006009
- InterPro:   IPR004276
- InterPro:   IPR007235 [H]

Pfam domain/function: PF04101 Glyco_tran_28_C; PF03033 Glyco_transf_28 [H]

EC number: =2.4.1.227 [H]

Molecular weight: Translated: 39507; Mature: 39507

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVFTGGGSAGHVTPNLAIIPYLKEDNWDISYIGSHQGIEKTIIEKEDIPYYSIASGK
CCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHCCCCCCCEEEECCCC
LRRYFDLKNIKDPFLVMKGVMDAYVRIRKLKPDVIFSKGGFVSVPVVIGGWLNRVPVLLH
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEHHHHCCHHHCCCEEEE
ESDMTPGLANKIALRFASKIFVTFEEAAKHLPKEKVIYTGSPVREEVLKGNREKALAFLG
CCCCCCCHHHHHHHHHHHHHHEEHHHHHHHCCCCCEEECCCHHHHHHHCCCHHHEEEEEC
FSRKKPVITIMGGSLGAKKINETVREALPELLRKYQIVHLCGKGNLDDSLQNKEGYRQFE
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHCCHHHHHHH
YVHGELPDILAITDFVISRAGSNAIFEFLTLQKPMLLIPLSKFASRGDQILNAESFERQG
HHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHCCCCHHCCCHHHHHCC
YASVLYEEDVTVNSLIKHVEELSHNNEAYKTALKKYNGKEAIQTIIHHISEA
CHHEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIVFTGGGSAGHVTPNLAIIPYLKEDNWDISYIGSHQGIEKTIIEKEDIPYYSIASGK
CCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHCCCCCCCEEEECCCC
LRRYFDLKNIKDPFLVMKGVMDAYVRIRKLKPDVIFSKGGFVSVPVVIGGWLNRVPVLLH
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEHHHHCCHHHCCCEEEE
ESDMTPGLANKIALRFASKIFVTFEEAAKHLPKEKVIYTGSPVREEVLKGNREKALAFLG
CCCCCCCHHHHHHHHHHHHHHEEHHHHHHHCCCCCEEECCCHHHHHHHCCCHHHEEEEEC
FSRKKPVITIMGGSLGAKKINETVREALPELLRKYQIVHLCGKGNLDDSLQNKEGYRQFE
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHCCHHHHHHH
YVHGELPDILAITDFVISRAGSNAIFEFLTLQKPMLLIPLSKFASRGDQILNAESFERQG
HHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHCCCCHHCCCHHHHHCC
YASVLYEEDVTVNSLIKHVEELSHNNEAYKTALKKYNGKEAIQTIIHHISEA
CHHEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]