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The map label for this gene is argF

Identifier: 49478550

GI number: 49478550

Start: 3958230

End: 3959180

Strand: Reverse

Name: argF

Synonym: BT9727_3875

Alternate gene names: 49478550

Gene position: 3959180-3958230 (Counterclockwise)

Preceding gene: 49480909

Following gene: 49478549

Centisome position: 75.59

GC content: 36.8

Gene sequence:

>951_bases
ATGTCAACTGTACAAGTACCGAAATTAAATACGAAAGATCTTTTAACACTAGAAGAATTAACGAAAGAAGAAATTATTTC
TTTAATTGAGTTCGCTATACATTTAAAAAAGAATAAGCAAGAGCCTTTATTACAAGGCAAAATATTAGGGCTTATTTTTG
ATAAGCATTCAACTCGTACTCGTGTATCTTTTGAAGCGGGAATGGTACAACTCGGGGGACATGGTATGTTTTTAAGTGGA
AAAGAGATGCAAATGGGAAGAGGAGAAACTGTTTCAGATACTGCGAAAGTATTATCGCAGTATATTGACGGGATTATGAT
ACGTACATTCTCGCACGCGGATGTAGAAGAGCTTGCAAAAGAATCGAGTATTCCTGTTATTAACGGTTTAACGGATGATC
ATCATCCTTGTCAAGCATTGGCAGACCTCATGACAATATATGAAGAAACAAATACATTTAAAGGAATAAAATTGGCTTAC
GTAGGTGACGGAAATAATGTATGTCATTCATTGTTGCTAGCGAGTGCAAAAGTCGGAATGCATATGACTGTTGCAACGCC
TGTAGGATATGAACCGAATGAAGAGATTGTAAAAAAAGCGTTAGCGATTGCCAAGGAAACAGGAGCTGAAATTGAAGTTT
TGCATGATCCTGAATTAGCGGTGAATGAAGCAGATTTCATTTATACTGACGTTTGGATGAGCATGGGGCAAGAGGGAGAA
GAAGAGAAATATACTTTATTTCAACCATATCAAATTAATAAAGAACTTGTTACGCATGCGAAGCAAACATATCGTTTCTT
ACACTGTTTACCTGCCCATCGTGAAGAAGAAGTAACAGGAGAAATTATAGATGGACCGCAGTCTATCGTTTTTGAGCAAG
CTGGTAATCGATTGCATGCGCAAAAAGCGTTATTAGTAAGTTTGTTTAAAAGTGTAGAAGAGCTTTCCTAA

Upstream 100 bases:

>100_bases
ACCACTTATTGTGACGAATGAAGAGTTAGAACAGGCAGTATATATAATAAAAAAAGTAGTTTGTACAAAAAACGTATCAA
TCATATAAGGGGGAAGTTTC

Downstream 100 bases:

>100_bases
AGGATGTACACCCCAGTTGCTAGATACGAATAACAACTGGGGTGTTTTTTATGGTTAAATTCTTTTTAGAAGAGAAAGTA
AATAGGATAAAAAGATATTA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSTVQVPKLNTKDLLTLEELTKEEIISLIEFAIHLKKNKQEPLLQGKILGLIFDKHSTRTRVSFEAGMVQLGGHGMFLSG
KEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAKESSIPVINGLTDDHHPCQALADLMTIYEETNTFKGIKLAY
VGDGNNVCHSLLLASAKVGMHMTVATPVGYEPNEEIVKKALAIAKETGAEIEVLHDPELAVNEADFIYTDVWMSMGQEGE
EEKYTLFQPYQINKELVTHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHAQKALLVSLFKSVEELS

Sequences:

>Translated_316_residues
MSTVQVPKLNTKDLLTLEELTKEEIISLIEFAIHLKKNKQEPLLQGKILGLIFDKHSTRTRVSFEAGMVQLGGHGMFLSG
KEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAKESSIPVINGLTDDHHPCQALADLMTIYEETNTFKGIKLAY
VGDGNNVCHSLLLASAKVGMHMTVATPVGYEPNEEIVKKALAIAKETGAEIEVLHDPELAVNEADFIYTDVWMSMGQEGE
EEKYTLFQPYQINKELVTHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHAQKALLVSLFKSVEELS
>Mature_315_residues
STVQVPKLNTKDLLTLEELTKEEIISLIEFAIHLKKNKQEPLLQGKILGLIFDKHSTRTRVSFEAGMVQLGGHGMFLSGK
EMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAKESSIPVINGLTDDHHPCQALADLMTIYEETNTFKGIKLAYV
GDGNNVCHSLLLASAKVGMHMTVATPVGYEPNEEIVKKALAIAKETGAEIEVLHDPELAVNEADFIYTDVWMSMGQEGEE
EKYTLFQPYQINKELVTHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHAQKALLVSLFKSVEELS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=308, Percent_Identity=44.8051948051948, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=29.0322580645161, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1790703, Length=324, Percent_Identity=37.962962962963, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=37.3088685015291, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=31.0679611650485, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994908, Length=358, Percent_Identity=25.9776536312849, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=27.5541795665635, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322373, Length=320, Percent_Identity=38.75, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=271, Percent_Identity=31.7343173431734, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=28.8025889967638, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35197; Mature: 35066

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVQVPKLNTKDLLTLEELTKEEIISLIEFAIHLKKNKQEPLLQGKILGLIFDKHSTRT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEEEEEEEECCCCCE
RVSFEAGMVQLGGHGMFLSGKEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAK
EEEEECCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
ESSIPVINGLTDDHHPCQALADLMTIYEETNTFKGIKLAYVGDGNNVCHSLLLASAKVGM
HCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCE
HMTVATPVGYEPNEEIVKKALAIAKETGAEIEVLHDPELAVNEADFIYTDVWMSMGQEGE
EEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCHHHHHHHHHHCCCCCC
EEKYTLFQPYQINKELVTHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHA
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHCCHHHH
QKALLVSLFKSVEELS
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STVQVPKLNTKDLLTLEELTKEEIISLIEFAIHLKKNKQEPLLQGKILGLIFDKHSTRT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEEEEEEEECCCCCE
RVSFEAGMVQLGGHGMFLSGKEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAK
EEEEECCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
ESSIPVINGLTDDHHPCQALADLMTIYEETNTFKGIKLAYVGDGNNVCHSLLLASAKVGM
HCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCE
HMTVATPVGYEPNEEIVKKALAIAKETGAEIEVLHDPELAVNEADFIYTDVWMSMGQEGE
EEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCHHHHHHHHHHCCCCCC
EEKYTLFQPYQINKELVTHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHA
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHCCHHHH
QKALLVSLFKSVEELS
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA