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The map label for this gene is mraW

Identifier: 49478448

GI number: 49478448

Start: 3761399

End: 3762331

Strand: Reverse

Name: mraW

Synonym: BT9727_3660

Alternate gene names: 49478448

Gene position: 3762331-3761399 (Counterclockwise)

Preceding gene: 49479388

Following gene: 49479387

Centisome position: 71.83

GC content: 37.51

Gene sequence:

>933_bases
ATGTTTAATCACGTAACAGTGCTTTTGAAGGAAACAGTAGATGGCTTAGATATAAAGCCAGATGGTACATATGTAGATTG
TACACTGGGGGGAGGAGGACATAGTTCTTATTTATTATCCCAATTAACTGAAGGCGGAAGATTAATCGCGTTTGATCAAG
ATGAGATAGCAATTCAAAATGCGAAAGAAAAATTCTCTTCATACGGTGAACAGTTCATAACAGTAAAGAGTAATTTCCGT
TACCTATCTGAAAAACTACAGGAATTAGGTATAACGGAAGTAGACGGTATTTTATTTGATTTAGGTGTTTCATCCCCGCA
ATTAGATACGCCAGAGCGTGGCTTTAGTTACCATCATGATGCACCGTTAGATATGCGAATGGATCAAGATGCCTCATTAA
CAGCATATGATGTTGTAAATAGTTGGTCATATGAACAACTTGTAAGAATTTTCTTCCAGTATGGCGAAGAGAAATTTTCA
AAACAAATAGCAAGGAAAATTGAAGCGTATCGTGAGAATAAAGCTATTGAAACGACAGGGGAATTAGTAGAACTAATTAA
AGAGGGTATTCCAGCTCCTGCTCGTAGAACGGGTGGACATCCTGCGAAGAGAGTGTTCCAAGCAATCCGTATTGCAGTAA
ATGATGAATTGAAAGTATTTGAAGAAGCGTTGGAATCTGCAATTGAGATGGTCAAGCCAGGCGGAAGAGTTAGTGTTATA
ACATTCCATTCTTTAGAAGACCGTATTTGCAAAACGACGTTTAAACGAAATAGTACAACGCCACAATTGCCGCCAGGATT
ACCAATTATTCCTGATGAATTTAAGCCGAAATTAAAGCTTATTACAAGAAAACCGATTTTACCTTCTGATATAGAGTTAG
AAGAAAATAATCGAGCGCGTTCTGCGAAATTGAGAATCGCTGAAAAACGATAA

Upstream 100 bases:

>100_bases
AGTCGTTCTTTCATAGTATTTCGTTCTCTAGTCGTGAAATATGCGTTTAGAAGAGGAGATACATTCAACATAATTAATTG
AAAAAAGAGTAGGTGCAACA

Downstream 100 bases:

>100_bases
AAAGGGGAGAGAGGTATGAGTAACTTAGCTGTAAAGTACAAACAACAAGCGCAAGAAGAGGTTCAGATTCAAACGCCCCC
ACAGCAGATGGTTCAGCCAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDASLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR

Sequences:

>Translated_310_residues
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDASLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR
>Mature_310_residues
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDASLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=34.9112426035503, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=37.3684210526316, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=45.5128205128205, Blast_Score=255, Evalue=3e-69,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=37.5757575757576, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=37.5757575757576, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34949; Mature: 34949

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQN
CCCHHEEHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
AKEKFSSYGEQFITVKSNFRYLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQDASLTAYDVVNSWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKAIETTG
CCCCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRAR
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCCC
SAKLRIAEKR
HHEEEEECCC
>Mature Secondary Structure
MFNHVTVLLKETVDGLDIKPDGTYVDCTLGGGGHSSYLLSQLTEGGRLIAFDQDEIAIQN
CCCHHEEHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
AKEKFSSYGEQFITVKSNFRYLSEKLQELGITEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQDASLTAYDVVNSWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKAIETTG
CCCCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRAR
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCCC
SAKLRIAEKR
HHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA