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The map label for this gene is fmt

Identifier: 49478422

GI number: 49478422

Start: 3704241

End: 3705185

Strand: Reverse

Name: fmt

Synonym: BT9727_3607

Alternate gene names: 49478422

Gene position: 3705185-3704241 (Counterclockwise)

Preceding gene: 49478423

Following gene: 49478421

Centisome position: 70.74

GC content: 38.2

Gene sequence:

>945_bases
ATGATAAAAGTAGTATTTATGGGAACACCGGACTTTTCAGTGCCGGTGCTTCGTCGTCTTATTGAAGATGGCTATGATGT
AATAGGTGTTGTGACGCAACCAGATCGTCCGGTAGGTAGAAAAAAAGTATTAACACCTACACCTGTCAAAGTGGAAGCAG
AAAAGCATGATATCCCGGTCTTACAGCCGTTAAGAATTCGTGAAAAAGACGAATATGAAAAAGTATTGGCATTAGAGCCA
GATTTAATTGTAACAGCTGCGTTCGGTCAAATTGTACCAAATGAAATTTTAGAAGCACCGAAGTATGGTTGTATTAACGT
CCACGCTTCTTTACTTCCTGAACTTCGTGGTGGCGCACCAATCCATTATGCAATTATGGAAGGGAAAGAAAAAACAGGTA
TTACAATTATGTATATGGTAGAAAAATTGGATGCTGGTGATATCTTAACACAAGTAGAAGTGGAAATTGAAGAGCGTGAA
ACGACAGGTTCGTTATTTGATAAGTTAAGTGAAGCAGGGGCACATCTTCTATCTAAAACAGTACCTTTACTAATTCAAGG
TAAGTTAGAACCAATTAAACAAAACGAGGAAGAAGTAACGTTTGCATATAATATAAAACGTGAACAAGAGAAAATTGATT
GGACAAAAACAGGTGAAGAAGTATACAATCACATTCGCGGATTGAATCCATGGCCAGTTGCTTATACAACTTTAGCAGGA
CAAGTTGTTAAAGTATGGTGGGGAGAAAAAGTACCTGTAACGAAATCAGCTGAAGCGGGTACGATTGTTGCGATTGAAGA
AGATGGTTTCGTTGTAGCAACTGGTAATGAAACGGGTGTTAAAATTACTGAATTGCAACCTTCTGGTAAAAAGCGTATGA
GTTGTTCGCAATTTTTACGTGGCACAAAACCTGAAATTGGAACGAAGTTAGGAGAAAATGCATGA

Upstream 100 bases:

>100_bases
ATGAAATCGATCATTTACACGGTGTGTTATTTACATCGAAAGTGACAAGATATTATGAGGAAAACGAATTAGAATAGGTA
TTAAAAGGAGCGGTTTTTAA

Downstream 100 bases:

>100_bases
GACAAAATGTTCGTGAATTAGCTCTTGATGGTTTAATTCAAGTAGAAAAAAGTGGTGCATACAGTAACTTACTTTTAAAT
AATCTAATTGAAAAAAGTAC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIKVVFMGTPDFSVPVLRRLIEDGYDVIGVVTQPDRPVGRKKVLTPTPVKVEAEKHDIPVLQPLRIREKDEYEKVLALEP
DLIVTAAFGQIVPNEILEAPKYGCINVHASLLPELRGGAPIHYAIMEGKEKTGITIMYMVEKLDAGDILTQVEVEIEERE
TTGSLFDKLSEAGAHLLSKTVPLLIQGKLEPIKQNEEEVTFAYNIKREQEKIDWTKTGEEVYNHIRGLNPWPVAYTTLAG
QVVKVWWGEKVPVTKSAEAGTIVAIEEDGFVVATGNETGVKITELQPSGKKRMSCSQFLRGTKPEIGTKLGENA

Sequences:

>Translated_314_residues
MIKVVFMGTPDFSVPVLRRLIEDGYDVIGVVTQPDRPVGRKKVLTPTPVKVEAEKHDIPVLQPLRIREKDEYEKVLALEP
DLIVTAAFGQIVPNEILEAPKYGCINVHASLLPELRGGAPIHYAIMEGKEKTGITIMYMVEKLDAGDILTQVEVEIEERE
TTGSLFDKLSEAGAHLLSKTVPLLIQGKLEPIKQNEEEVTFAYNIKREQEKIDWTKTGEEVYNHIRGLNPWPVAYTTLAG
QVVKVWWGEKVPVTKSAEAGTIVAIEEDGFVVATGNETGVKITELQPSGKKRMSCSQFLRGTKPEIGTKLGENA
>Mature_314_residues
MIKVVFMGTPDFSVPVLRRLIEDGYDVIGVVTQPDRPVGRKKVLTPTPVKVEAEKHDIPVLQPLRIREKDEYEKVLALEP
DLIVTAAFGQIVPNEILEAPKYGCINVHASLLPELRGGAPIHYAIMEGKEKTGITIMYMVEKLDAGDILTQVEVEIEERE
TTGSLFDKLSEAGAHLLSKTVPLLIQGKLEPIKQNEEEVTFAYNIKREQEKIDWTKTGEEVYNHIRGLNPWPVAYTTLAG
QVVKVWWGEKVPVTKSAEAGTIVAIEEDGFVVATGNETGVKITELQPSGKKRMSCSQFLRGTKPEIGTKLGENA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=322, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI238814322, Length=314, Percent_Identity=27.7070063694268, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=30.9322033898305, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1789683, Length=302, Percent_Identity=41.3907284768212, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788589, Length=276, Percent_Identity=27.8985507246377, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI133930964, Length=325, Percent_Identity=32, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319458, Length=278, Percent_Identity=26.978417266187, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550868, Length=306, Percent_Identity=32.3529411764706, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI28571984, Length=283, Percent_Identity=31.8021201413428, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585660, Length=245, Percent_Identity=26.9387755102041, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34795; Mature: 34795

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVVFMGTPDFSVPVLRRLIEDGYDVIGVVTQPDRPVGRKKVLTPTPVKVEAEKHDIPV
CEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCC
LQPLRIREKDEYEKVLALEPDLIVTAAFGQIVPNEILEAPKYGCINVHASLLPELRGGAP
CCCCCCCCCCCHHHEEEECCCEEEEEHHHHCCCHHHHCCCCCCEEEEHHHHHHHHCCCCC
IHYAIMEGKEKTGITIMYMVEKLDAGDILTQVEVEIEERETTGSLFDKLSEAGAHLLSKT
EEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCC
VPLLIQGKLEPIKQNEEEVTFAYNIKREQEKIDWTKTGEEVYNHIRGLNPWPVAYTTLAG
CCEEEECCCCCCCCCCCEEEEEEEECHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHC
QVVKVWWGEKVPVTKSAEAGTIVAIEEDGFVVATGNETGVKITELQPSGKKRMSCSQFLR
EEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHC
GTKPEIGTKLGENA
CCCCCHHCCCCCCC
>Mature Secondary Structure
MIKVVFMGTPDFSVPVLRRLIEDGYDVIGVVTQPDRPVGRKKVLTPTPVKVEAEKHDIPV
CEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCC
LQPLRIREKDEYEKVLALEPDLIVTAAFGQIVPNEILEAPKYGCINVHASLLPELRGGAP
CCCCCCCCCCCHHHEEEECCCEEEEEHHHHCCCHHHHCCCCCCEEEEHHHHHHHHCCCCC
IHYAIMEGKEKTGITIMYMVEKLDAGDILTQVEVEIEERETTGSLFDKLSEAGAHLLSKT
EEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCC
VPLLIQGKLEPIKQNEEEVTFAYNIKREQEKIDWTKTGEEVYNHIRGLNPWPVAYTTLAG
CCEEEECCCCCCCCCCCEEEEEEEECHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHC
QVVKVWWGEKVPVTKSAEAGTIVAIEEDGFVVATGNETGVKITELQPSGKKRMSCSQFLR
EEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHC
GTKPEIGTKLGENA
CCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA