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The map label for this gene is rnjB [H]

Identifier: 49478379

GI number: 49478379

Start: 3630819

End: 3632489

Strand: Reverse

Name: rnjB [H]

Synonym: BT9727_3538

Alternate gene names: 49478379

Gene position: 3632489-3630819 (Counterclockwise)

Preceding gene: 49478381

Following gene: 49479417

Centisome position: 69.35

GC content: 36.39

Gene sequence:

>1671_bases
ATGAAGAGAAAAGAGAATGAGTCTGTTAAAGTATTTGCTCTTGGTGGAGTAGGTGAAATCGGGAAAAACATGTACTGTGT
TGAAATTGATTCTGAAATCTTTATTGTAGATGCAGGGTTAATGTTCCCGGGAGATGAAATGTTTGGAATTGATATCGTTA
TTCCTGATATTACATATTTAGTGGAAAACCAAGAGCGAGTAAAAGGTTTATTTATTACTCACGGTCATGAAGATCATATT
GGTGGTATTGTTTACGTACTTCGTAAATTATCTATTCCAGTGTATGCAACGAAATTAACAGTAGGACTTATACAAGAAAA
ACTTGGCGAAGCGGGTATGTTAGGCCGTGTAGACTTAAAAACAATCGATTCAAATTCAACAGTAGAGTTTAATACTACAA
CGGTGTCATTCTTTGGAACGACACATAGTATTCCAGATTCTGTTGGTGTTTGTTTCCATACATCAAAAGGTGCGATCGTA
TATACGGGAGACTTTAAATTCGATCAAACTCCAATTGGAAATAGTGGAGCAGATCTTGGGAAAATGGCTCAAATCGGAAA
TGAAGGTGTACTATGCCTATTATCTGATAGTACAAATGCTGAGCGTCCAGGCTATACAGGTTCTGAAAAAGAAGTTGGTG
TAGAAATTTCTAAAGTATTCTACGGCGCAGAAGGACGTATTATCGTTGCTTCATTTGCATCGAATGTACATCGTATTCAA
CAAGTATTTGATGCAGCTGCTGAAACAGGACGAAAAGTCGCGGTTGTAGGACGTAGTATGGTGAAAGTAGTAGATATCGC
AAGACGTCTTGGTTATTTAGATGTTCCAGAAGGTATGGTTATTTCACTACAAGAAGTAGACAATTTCCCAGAGAAAAAGG
TAGCTATTTTAACGACAGGTAGCCAAGGCGAACCGATGGCAGCCCTTTCTAGAATGGCGAAGCAAGCTCATAAACAAATT
TCAATTCGTAAAGGTGATACGGTTATTATTGCCGCTTCTCCAATTCCGGGTAATGAAATATCTGTATCGAAAATTATTGA
CTTGTTATTTAGAGCTGGAGCTGAAGTTGTTTATTATGGTGAAAGAAAAGTTCACGTTTCAGGTCACGGTAGCCAAGAAG
AATTAAAACTAATGTTAAACTTAATGAAACCGAAATATTTTGTACCCGTACATGGTGAGTTTCGTATGCAAAAAGCACAT
GCATACTTAGCTGAAGATGTAGGCATTGCAAGAGAAAATATCTTTATCGTAGAAAAAGGCGATGTAATTGGTTTTGGTGA
CGATGAAGCAAGCTTAGTTGGTAAAGTGCAAGCGGGCAATGTATTAATTGATGGATTAGGTGTAGGTGACGTTGGTAATA
TCGTTCTTCGAGATAGAAAAATGTTATCTCAAGATGGAATATTAGTAGTTGTTGTAACACTTGGTAAAGATGAAAAGAAA
ATTATCTCTGGTCCAGAAATCATTTCACGTGGATTTGTATATGTTCGTGAATCAGAAGCGTTAATTGACCGTTCTACAGA
GATTGTACGTATGATTGTTGAGCAATCAATTAAAGAATATTCAATTGAATGGTCTATGTTAAAACAAAATATTCGTGAAT
TATTAGGACAGTTCTTGTACGAAGAGACAAAGAGAAAACCTATGATTTTACCGATTATTATGGAAGTATAA

Upstream 100 bases:

>100_bases
GTCTTGGACGGTGAATATATGGTGGTTGTAGAGTTTCCCCTGGTGTCTCTGCGATTTTAGTGAAATCAGTGTTTGAAAGA
TTTTTAAACCAGGCAACAAC

Downstream 100 bases:

>100_bases
AAAAGATCACCTTTACGGGTGATCTTTTTTCTTTTTAAACTCTATCGAATGAAATTTATAACTTAAGAAATAATAGTGAT
ATACGAGTGAAAGGGGATGC

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYLVENQERVKGLFITHGHEDHI
GGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIV
YTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEVDNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQI
SIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAH
AYLAEDVGIARENIFIVEKGDVIGFGDDEASLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
IISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV

Sequences:

>Translated_556_residues
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYLVENQERVKGLFITHGHEDHI
GGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIV
YTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEVDNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQI
SIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAH
AYLAEDVGIARENIFIVEKGDVIGFGDDEASLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
IISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV
>Mature_556_residues
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYLVENQERVKGLFITHGHEDHI
GGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIV
YTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEVDNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQI
SIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAH
AYLAEDVGIARENIFIVEKGDVIGFGDDEASLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
IISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 60893; Mature: 60893

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEEECEECCCCCEEEEEEEECCEEEE
VENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLK
ECCCHHHCEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEEE
TIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLG
EECCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCEECCCCCCCCCCCHH
KMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHH
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEVDNFPEKKVAILTTG
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEECCCCCEEEHHHHCCCCCCEEEEEEEC
SQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYG
CCCCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEEEC
ERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGIARENIFIVEKG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCEEEEECC
DVIGFGDDEASLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
CEEEECCCCCCEEEEEEECCEEEEECCCCCCCCEEEECHHHHCCCCEEEEEEEECCCCHH
IISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLY
HCCCCHHHHCCEEEEECCHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EETKRKPMILPIIMEV
HHHCCCCEEEEEEEEC
>Mature Secondary Structure
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEEECEECCCCCEEEEEEEECCEEEE
VENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLK
ECCCHHHCEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEEE
TIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAIVYTGDFKFDQTPIGNSGADLG
EECCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCEECCCCCCCCCCCHH
KMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHH
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLDVPEGMVISLQEVDNFPEKKVAILTTG
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEECCCCCEEEHHHHCCCCCCEEEEEEEC
SQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYG
CCCCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEEEC
ERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGIARENIFIVEKG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCEEEEECC
DVIGFGDDEASLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
CEEEECCCCCCEEEEEEECCEEEEECCCCCCCCEEEECHHHHCCCCEEEEEEEECCCCHH
IISGPEIISRGFVYVRESEALIDRSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLY
HCCCCHHHHCCEEEEECCHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EETKRKPMILPIIMEV
HHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]