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The map label for this gene is cwlH [H]

Identifier: 49478176

GI number: 49478176

Start: 3179718

End: 3181337

Strand: Reverse

Name: cwlH [H]

Synonym: BT9727_3111

Alternate gene names: 49478176

Gene position: 3181337-3179718 (Counterclockwise)

Preceding gene: 49478177

Following gene: 49478175

Centisome position: 60.74

GC content: 37.22

Gene sequence:

>1620_bases
ATGAGTATTAAATTGAATAAAAAAGTAGTAGCATTAACAGCAGGACTAACGGTATTATCTTCATCGATGGTATCGTTCGC
AGAAGAACAGCCATCTGTAAATAACCATCAATCTATAAGTATTCAGGAACAAAGCGGGACAGATCAACTTCAAGTATCAG
AAGAACAAGTTTTAAAAAGTATTGAAGAAAGTTTTGAAGGGGTAGATGGACGCGGTGGAGGTACTGTCAATAATAAGAAA
GTGGACAGTCTTCAAAGTTCAGAAGGTATTTATGAAGAAACGGGTACATTAACGGTTCCTCACAATTCAAATAGAACAAA
GCGTAGTCTTGCTTTCTCAGCACCTACAGTTTCTAGTACGATAAACGGGGTTCCATTCACGGAATGGATTGTTCCAACAG
GTAACGATAATATTCGCCCTCAAAATCATATGATTCCAAAGTATATTACAATTCATGAGACTGATAATAAAAGCGTAGGA
GCAGGAGCTAAAAGCCACGCTCAATACTTATATAACCAAGCAGTAGGAGAAACAGACCGAGCTGCATCATGGCACTTTAC
AGTAGATGATAAAGAAATTTATCAACACTTACCATTGAACGAGAATGGATGGCACGCAGGAGATGGTGATGGGCCGGGTA
ATAGAGAATCTATTGCAATTGAAATTGCAGTTAATAGTGATGGTGACTACAATAAGGCAGTCGATAACGCTAAGAAACTT
GCAGCTTATTTAATGAAAGAAACGGGTGTACCATTAAACAACATCGTAAAACATCAAAAATGGAGTGGTAAAAACTGTCC
CGATATGTTGATTAATAGTGGACAATGGGTACCATTCGTAAATGGCATTGAAGGATATTATAATAAATTTTATCAACCGA
CAGATGATATAACAGGAGGTTGGTATGAATCGGCTATTCGTGATTTGAACAGAAGAGGTATTATGGTAGGAGAAGGAAAC
GGCATTTTTGCGCCTAATAGATCTGTAACAAGAGCTGAGTTTGCGCAATTAATCTCGAATTCTTTAAGTCTTCCAGCTGG
AGATGCGACGTTTAAAGATTTAAATGATGTAAATTCAACTTTACGAGATGGTATTAAACGTACTGCAAGTGCAGGCATCA
TTTCTGGTAGAGGAGACGGTAATTTTGACCCTAACACACCTATTACAAGAGAAGAAGCAGCGGTTATTGTAAATAAAGCT
TTACAGTACAAAGGAATAAATGGACCATTGGTAAACTTACCATTTACAGATAAAGGTCAAATTATCTATAAAGAAGCTGT
ACAAAGATTATACGGCTTAGGTATTGTAACAGGTATGGGAGATAACCTATATGTCCCTAAAGGAACAACAACTCGTGGTG
AAACTGCAGCATTCATTTTAAACATGCTTCAAGTTATTGAAACTGGGAATGTTCAAAAAGGAATTGGTACGGTTGAAATA
AATGGTATTGGAGTAAATGTTCGCAGCGGAGCAGGATCTAGCTACTCTGTTGTTAGAAAAGCAAGCAAAGGTGAAAAAGC
TACAGTATACGAAGAAAAGAATGGTTGGCTAAGAATTGGTACAGGTGAGTGGGTTTATAATGAGCCTAGCTACATCGTCT
ATACGAAAGATAAAAATTAA

Upstream 100 bases:

>100_bases
ATGAAAAATGAAGGTGTTATGTAACTATTAGGATGTACATTAGAAGAAGTGTGAGGAGGAAGGTATATCTATTAAAATAG
CAAGATATACTAAATTAAAT

Downstream 100 bases:

>100_bases
ATAGTTACGAGTAAGTCATACATCACATATCTCATGTATTCCCAGGCAATGGACAGCATTTTCTCCATTGCCTGTTTTTG
AGTTTCAGTTTTTTCGTAAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSIKLNKKVVALTAGLTVLSSSMVSFAEEQPSVNNHQSISIQEQSGTDQLQVSEEQVLKSIEESFEGVDGRGGGTVNNKK
VDSLQSSEGIYEETGTLTVPHNSNRTKRSLAFSAPTVSSTINGVPFTEWIVPTGNDNIRPQNHMIPKYITIHETDNKSVG
AGAKSHAQYLYNQAVGETDRAASWHFTVDDKEIYQHLPLNENGWHAGDGDGPGNRESIAIEIAVNSDGDYNKAVDNAKKL
AAYLMKETGVPLNNIVKHQKWSGKNCPDMLINSGQWVPFVNGIEGYYNKFYQPTDDITGGWYESAIRDLNRRGIMVGEGN
GIFAPNRSVTRAEFAQLISNSLSLPAGDATFKDLNDVNSTLRDGIKRTASAGIISGRGDGNFDPNTPITREEAAVIVNKA
LQYKGINGPLVNLPFTDKGQIIYKEAVQRLYGLGIVTGMGDNLYVPKGTTTRGETAAFILNMLQVIETGNVQKGIGTVEI
NGIGVNVRSGAGSSYSVVRKASKGEKATVYEEKNGWLRIGTGEWVYNEPSYIVYTKDKN

Sequences:

>Translated_539_residues
MSIKLNKKVVALTAGLTVLSSSMVSFAEEQPSVNNHQSISIQEQSGTDQLQVSEEQVLKSIEESFEGVDGRGGGTVNNKK
VDSLQSSEGIYEETGTLTVPHNSNRTKRSLAFSAPTVSSTINGVPFTEWIVPTGNDNIRPQNHMIPKYITIHETDNKSVG
AGAKSHAQYLYNQAVGETDRAASWHFTVDDKEIYQHLPLNENGWHAGDGDGPGNRESIAIEIAVNSDGDYNKAVDNAKKL
AAYLMKETGVPLNNIVKHQKWSGKNCPDMLINSGQWVPFVNGIEGYYNKFYQPTDDITGGWYESAIRDLNRRGIMVGEGN
GIFAPNRSVTRAEFAQLISNSLSLPAGDATFKDLNDVNSTLRDGIKRTASAGIISGRGDGNFDPNTPITREEAAVIVNKA
LQYKGINGPLVNLPFTDKGQIIYKEAVQRLYGLGIVTGMGDNLYVPKGTTTRGETAAFILNMLQVIETGNVQKGIGTVEI
NGIGVNVRSGAGSSYSVVRKASKGEKATVYEEKNGWLRIGTGEWVYNEPSYIVYTKDKN
>Mature_538_residues
SIKLNKKVVALTAGLTVLSSSMVSFAEEQPSVNNHQSISIQEQSGTDQLQVSEEQVLKSIEESFEGVDGRGGGTVNNKKV
DSLQSSEGIYEETGTLTVPHNSNRTKRSLAFSAPTVSSTINGVPFTEWIVPTGNDNIRPQNHMIPKYITIHETDNKSVGA
GAKSHAQYLYNQAVGETDRAASWHFTVDDKEIYQHLPLNENGWHAGDGDGPGNRESIAIEIAVNSDGDYNKAVDNAKKLA
AYLMKETGVPLNNIVKHQKWSGKNCPDMLINSGQWVPFVNGIEGYYNKFYQPTDDITGGWYESAIRDLNRRGIMVGEGNG
IFAPNRSVTRAEFAQLISNSLSLPAGDATFKDLNDVNSTLRDGIKRTASAGIISGRGDGNFDPNTPITREEAAVIVNKAL
QYKGINGPLVNLPFTDKGQIIYKEAVQRLYGLGIVTGMGDNLYVPKGTTTRGETAAFILNMLQVIETGNVQKGIGTVEIN
GIGVNVRSGAGSSYSVVRKASKGEKATVYEEKNGWLRIGTGEWVYNEPSYIVYTKDKN

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 58593; Mature: 58462

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01072 SLH_DOMAIN ; PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLNKKVVALTAGLTVLSSSMVSFAEEQPSVNNHQSISIQEQSGTDQLQVSEEQVLKS
CCEEECCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEECHHHHHHH
IEESFEGVDGRGGGTVNNKKVDSLQSSEGIYEETGTLTVPHNSNRTKRSLAFSAPTVSST
HHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEECCCEEEECCCCCCCHHEEEECCCCHHHH
INGVPFTEWIVPTGNDNIRPQNHMIPKYITIHETDNKSVGAGAKSHAQYLYNQAVGETDR
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
AASWHFTVDDKEIYQHLPLNENGWHAGDGDGPGNRESIAIEIAVNSDGDYNKAVDNAKKL
CCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
AAYLMKETGVPLNNIVKHQKWSGKNCPDMLINSGQWVPFVNGIEGYYNKFYQPTDDITGG
HHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHCCCCCCEECCCHHHHHHHCCCCCCCCCCH
WYESAIRDLNRRGIMVGEGNGIFAPNRSVTRAEFAQLISNSLSLPAGDATFKDLNDVNST
HHHHHHHHHHHCEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRDGIKRTASAGIISGRGDGNFDPNTPITREEAAVIVNKALQYKGINGPLVNLPFTDKGQ
HHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
IIYKEAVQRLYGLGIVTGMGDNLYVPKGTTTRGETAAFILNMLQVIETGNVQKGIGTVEI
CHHHHHHHHHHCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
NGIGVNVRSGAGSSYSVVRKASKGEKATVYEEKNGWLRIGTGEWVYNEPSYIVYTKDKN
EEEEEEEECCCCCCHHHHHHCCCCCCEEEEECCCCEEEECCCCEEECCCCEEEEECCCC
>Mature Secondary Structure 
SIKLNKKVVALTAGLTVLSSSMVSFAEEQPSVNNHQSISIQEQSGTDQLQVSEEQVLKS
CEEECCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEECHHHHHHH
IEESFEGVDGRGGGTVNNKKVDSLQSSEGIYEETGTLTVPHNSNRTKRSLAFSAPTVSST
HHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEECCCEEEECCCCCCCHHEEEECCCCHHHH
INGVPFTEWIVPTGNDNIRPQNHMIPKYITIHETDNKSVGAGAKSHAQYLYNQAVGETDR
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
AASWHFTVDDKEIYQHLPLNENGWHAGDGDGPGNRESIAIEIAVNSDGDYNKAVDNAKKL
CCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
AAYLMKETGVPLNNIVKHQKWSGKNCPDMLINSGQWVPFVNGIEGYYNKFYQPTDDITGG
HHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHCCCCCCEECCCHHHHHHHCCCCCCCCCCH
WYESAIRDLNRRGIMVGEGNGIFAPNRSVTRAEFAQLISNSLSLPAGDATFKDLNDVNST
HHHHHHHHHHHCEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LRDGIKRTASAGIISGRGDGNFDPNTPITREEAAVIVNKALQYKGINGPLVNLPFTDKGQ
HHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
IIYKEAVQRLYGLGIVTGMGDNLYVPKGTTTRGETAAFILNMLQVIETGNVQKGIGTVEI
CHHHHHHHHHHCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
NGIGVNVRSGAGSSYSVVRKASKGEKATVYEEKNGWLRIGTGEWVYNEPSYIVYTKDKN
EEEEEEEECCCCCCHHHHHHCCCCCCEEEEECCCCEEEECCCCEEECCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]