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The map label for this gene is yvaE [H]

Identifier: 49478159

GI number: 49478159

Start: 3163060

End: 3163422

Strand: Reverse

Name: yvaE [H]

Synonym: BT9727_3093

Alternate gene names: 49478159

Gene position: 3163422-3163060 (Counterclockwise)

Preceding gene: 49478160

Following gene: 49478158

Centisome position: 60.4

GC content: 31.96

Gene sequence:

>363_bases
GTGCAGTGGATTTATTTATGCTTAGCAATTTTATTTGAGGTAGCAGGTACAACTGCGATGAAATTATCTGATGGATTAAC
GAAACTAGTACCAAGCATTCTTATTTTTGTATTTTATGTAGTAAGTTTTGCATTTCTTTCATTTGCATTAAAAGGGATGG
AAATGTCCGTTGCATATGCGGTTTGGTCAGGTATGGGAATTGTAACGATTACAACAATTGGTTTTATTTATTTTGGAGAA
AGCATCAATGTAATTAAGATACTAGCAATTCTTTTTATTTTAGTTGGAGTCGTAATATTAAATTTTAATAGTGCGGCACA
TGAAACGAAGAAAAAAGAAATGGTGGAGGAACGTATACATTGA

Upstream 100 bases:

>100_bases
TTATTGAAAAAAGTGATCATACATTAGGAAGTATGCTGGAATCTATTATGGATATGTTATTGTATGGCATTTCTAATTCG
AAATAGAAGGTGGGAGAGTA

Downstream 100 bases:

>100_bases
ATCGATTAAAGTATTTTTTCCTTATTACTGATGTTGGGTTTGTTATATATTGGCTCATAACAATTTTTCATATAATTCCG
CAAGAATATTTATTTAAAGA

Product: SMR family multidrug efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MQWIYLCLAILFEVAGTTAMKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYAVWSGMGIVTITTIGFIYFGE
SINVIKILAILFILVGVVILNFNSAAHETKKKEMVEERIH

Sequences:

>Translated_120_residues
MQWIYLCLAILFEVAGTTAMKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYAVWSGMGIVTITTIGFIYFGE
SINVIKILAILFILVGVVILNFNSAAHETKKKEMVEERIH
>Mature_120_residues
MQWIYLCLAILFEVAGTTAMKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYAVWSGMGIVTITTIGFIYFGE
SINVIKILAILFILVGVVILNFNSAAHETKKKEMVEERIH

Specific function: Multidrug Transporter. Confers Resistance To A Wide Range Of Toxic Compounds By Removing Them For The Cells. The Efflux Is Coupled To An Influx Of Protons. Probably Prevents The Incorporation Of Methyl VIologen Into Cells. Involved In Ethidium Bromide Ef

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=108, Percent_Identity=40.7407407407407, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI87082378, Length=104, Percent_Identity=33.6538461538462, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787884, Length=112, Percent_Identity=36.6071428571429, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1787883, Length=97, Percent_Identity=32.9896907216495, Blast_Score=63, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 13348; Mature: 13348

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWIYLCLAILFEVAGTTAMKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWSGMGIVTITTIGFIYFGESINVIKILAILFILVGVVILNFNSAAHETKKKEMVEERIH
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQWIYLCLAILFEVAGTTAMKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWSGMGIVTITTIGFIYFGESINVIKILAILFILVGVVILNFNSAAHETKKKEMVEERIH
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]