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The map label for this gene is nfi

Identifier: 49478135

GI number: 49478135

Start: 3138284

End: 3138991

Strand: Reverse

Name: nfi

Synonym: BT9727_3063

Alternate gene names: 49478135

Gene position: 3138991-3138284 (Counterclockwise)

Preceding gene: 49478136

Following gene: 49478134

Centisome position: 59.93

GC content: 29.8

Gene sequence:

>708_bases
GTGATGATAATGAATAATTTTGATGAAAAGAAATTAATACAAGAGTTTTCAAAAATCCAAAGTGAATTATCACCTAGAAT
TAAATTAGAAAATAATTTTCAAATAGAAGAGATACAATTTATCGCAGGGGTAGATTTGGCTTATTGGAATGTGAATAAAA
CTAAGTATGGTACATGTTGTATTGTAATCATTGATTATGGTACAAAGGAAGTTGTCGAAAAAGTATACAGTTATGGAGAA
ATACCAATCCCGTATATTCCAGGTTTCCTTGCGTTTAGAGAGTTACCTTTAATAATAGAGGCAGTTAAAAAGCTAACAAA
GCAACCAGATATATATTTTTTTGATGGTAATGGATATTTACATTATCGTCACATGGGTATTGCTACTCATGCTTCCTTTC
TGCTAAATAAACCAACCATTGGTGTAGCAAAAAGTTATCTTAAAATTCGAGATGTAGATTTTATTATGCCAGAGAATCTA
AAAGGATCTTACACTGATATTGTAATAAATAATGAAGTCTATGGTCGAACTTTAAGAACGACCAAAAATGTTAAACCTAT
TTTTGTTTCTTGTGGGAACTGGATTGATTTAGATACAAGTACTGAAATTGTTATGAACTGTATTAATAATGAAAGTCGTT
TACCTATTCCTGTTAGATTAGCAGATTTAGAAACACATAAAATGAGGAAGAAGTTAATGGAAGGATAA

Upstream 100 bases:

>100_bases
TGTTTAAAATATACTGAATGGGATAAACAAGGGAATATAATTAGGCAAAAAGTTGCTCCAACAAAAGAGGAATTAAGACT
AATTACCCGATTAGCTGATT

Downstream 100 bases:

>100_bases
AATAGTGTATTCTATATTTATTAAGCAATAAATACAAGTAGAATGCATTTATATAAACTTGTAAGTTTTACCTAGTATTT
GTCACAACATGTAAAGGCTG

Product: deoxyinosine 3'endonuclease (endonuclease V)

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MMIMNNFDEKKLIQEFSKIQSELSPRIKLENNFQIEEIQFIAGVDLAYWNVNKTKYGTCCIVIIDYGTKEVVEKVYSYGE
IPIPYIPGFLAFRELPLIIEAVKKLTKQPDIYFFDGNGYLHYRHMGIATHASFLLNKPTIGVAKSYLKIRDVDFIMPENL
KGSYTDIVINNEVYGRTLRTTKNVKPIFVSCGNWIDLDTSTEIVMNCINNESRLPIPVRLADLETHKMRKKLMEG

Sequences:

>Translated_235_residues
MMIMNNFDEKKLIQEFSKIQSELSPRIKLENNFQIEEIQFIAGVDLAYWNVNKTKYGTCCIVIIDYGTKEVVEKVYSYGE
IPIPYIPGFLAFRELPLIIEAVKKLTKQPDIYFFDGNGYLHYRHMGIATHASFLLNKPTIGVAKSYLKIRDVDFIMPENL
KGSYTDIVINNEVYGRTLRTTKNVKPIFVSCGNWIDLDTSTEIVMNCINNESRLPIPVRLADLETHKMRKKLMEG
>Mature_235_residues
MMIMNNFDEKKLIQEFSKIQSELSPRIKLENNFQIEEIQFIAGVDLAYWNVNKTKYGTCCIVIIDYGTKEVVEKVYSYGE
IPIPYIPGFLAFRELPLIIEAVKKLTKQPDIYFFDGNGYLHYRHMGIATHASFLLNKPTIGVAKSYLKIRDVDFIMPENL
KGSYTDIVINNEVYGRTLRTTKNVKPIFVSCGNWIDLDTSTEIVMNCINNESRLPIPVRLADLETHKMRKKLMEG

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=210, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI87082357, Length=203, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71981509, Length=225, Percent_Identity=25.7777777777778, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_BACHK (Q6HGE0)

Other databases:

- EMBL:   AE017355
- RefSeq:   YP_037386.1
- ProteinModelPortal:   Q6HGE0
- EnsemblBacteria:   EBBACT00000073919
- GeneID:   2858351
- GenomeReviews:   AE017355_GR
- KEGG:   btk:BT9727_3063
- NMPDR:   fig|281309.1.peg.3026
- GeneTree:   EBGT00050000000793
- HOGENOM:   HBG716360
- OMA:   CMRGYRL
- ProtClustDB:   CLSK882396
- BioCyc:   BTHU281309:BT9727_3063-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 27131; Mature: 27131

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIMNNFDEKKLIQEFSKIQSELSPRIKLENNFQIEEIQFIAGVDLAYWNVNKTKYGTCC
CCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEECCEEEEEECCCCCCCEEE
IVIIDYGTKEVVEKVYSYGEIPIPYIPGFLAFRELPLIIEAVKKLTKQPDIYFFDGNGYL
EEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
HYRHMGIATHASFLLNKPTIGVAKSYLKIRDVDFIMPENLKGSYTDIVINNEVYGRTLRT
EEEECCHHHHHHHHHCCCCHHHHHHHHEEECEEEECCCCCCCCEEEEEECCCCCCCEEEC
TKNVKPIFVSCGNWIDLDTSTEIVMNCINNESRLPIPVRLADLETHKMRKKLMEG
CCCCCEEEEECCCEEECCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MMIMNNFDEKKLIQEFSKIQSELSPRIKLENNFQIEEIQFIAGVDLAYWNVNKTKYGTCC
CCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEECCEEEEEECCCCCCCEEE
IVIIDYGTKEVVEKVYSYGEIPIPYIPGFLAFRELPLIIEAVKKLTKQPDIYFFDGNGYL
EEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
HYRHMGIATHASFLLNKPTIGVAKSYLKIRDVDFIMPENLKGSYTDIVINNEVYGRTLRT
EEEECCHHHHHHHHHCCCCHHHHHHHHEEECEEEECCCCCCCCEEEEEECCCCCCCEEEC
TKNVKPIFVSCGNWIDLDTSTEIVMNCINNESRLPIPVRLADLETHKMRKKLMEG
CCCCCEEEEECCCEEECCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA