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The map label for this gene is glgB

Identifier: 49477291

GI number: 49477291

Start: 4649050

End: 4650987

Strand: Reverse

Name: glgB

Synonym: BT9727_4599

Alternate gene names: 49477291

Gene position: 4650987-4649050 (Counterclockwise)

Preceding gene: 49476897

Following gene: 49478799

Centisome position: 88.8

GC content: 37.56

Gene sequence:

>1938_bases
TTGAGTGTAATAAATTGTGAAGAAGTGAAACGAGATGAGTTTCATACCGAAAAGTACTATGAAAGTTATAACATCTTTGG
CGCACATATTGTGACGGAAGATGAGATGCGAGGTGTACGATTTACAGTATGGGCTCCTCATGCGAAAGCAATGAGTGTTG
TTGGAGATTTTAATGAATGGGATTATGAGCAACATAAGATGCTGCAAGTGACAGAAGAGGGAATTTGGTCCCTATTTATA
CCGCATATAGAAGAAAGAGAAATATATAAATATGCGATTGAAACGATGGCTGGTGATGTCATTTTTAAGGCAGATCCGTA
TGCTGTGTATGCAGAAGTAAGACCGAATACGGCATCTGTAGTTTTTGATATAAAGGGATATGAATGGAATGATAAAAACT
GGAGCCGTAAGAAAAAGAAAAAATCGGTTTATAAAGAAGCGATGACAGTTTATGAATTACATTTCGGTTCTTGGAAAAAG
AAAGAAGATGGAACACTGTATTCTTACAGAGAAATGGCAGAAGAACTCATTCCTTATGTGGTGGAGCATCAATTTACACA
TATTGAAATTATGCCGCTTGTTGAGCATCCATATGATCGTTCTTGGGGATATCAAGGGACGGGATATTATGCAGCAACGA
GTAGATTCGGCACGCCATATGATTTGATGCATTTTGTCGATGAATGTCATAAATATGGAATCGGTGTCATTTTAGATTGG
GTGCCGGGGCATTTTTGTAAAGATGCTCACGGTTTATATTTGTTTGATGGGACACCGACTTATGAATATAAAGATAAAGA
TGTGCAAGAAAATCCAGTATGGGGAACTGTCAACTTTGATTTAGGAAAGAGGGAAGTACGTAATTTCTTAATTTCAAATG
CGTTATTTTGGATGAGATATTTCCATATTGATGGTTTCAGGGTGGATGCAGTTGCGAACATGCTGTACTGGAATAAAGAA
GGACAAGAGCAAAGTAATGAGCACGCTGTGTCATTTTTACGAGAGTTAAATGAAGCGGTGTTTGCAGAGGATGAAGATTT
TCTTATGACAGCAGAGGACTCAACAGCTTGGCCACTTGTAACAGCTCCAACGTATGAAGGTGGGCTTGGATTCAATTACA
AATGGAATATGGGCTGGATGAATGACGTGCTGAAATATATGGAATGTGCGCCAGAGTACAGAAAATATATTCATGATAAA
ATGACGTTCTCTTTACTATATGCTTACTCTGAAAATTTCATATTACCGCTTTCTCATGATGAAGTCGTTCATGGGAAAAA
GTCGTTATTAAATAAAATGCCAGGAGACTACTGGGATAAGTTTGCTCAACTTCGTTTATTATATGGATATTTCTTTACTC
ACCCAGGAAAGAAATTACTTTTCATGGGAGGAGAATTCGGGCAGTTTGATGAGTGGAAAGACCTTGAAGATTTAGATTGG
AATTTACATGATTTTGAAATGCATCGTTATATGCATGATTACTTTAAAGAGCTCATAGCATTGTATAAGCGCTCTAAACC
ACTTTGGCAGCTTGATCATTCGCGTGAAGGATTTCAGTGGATTGATGCTAATAATAATGAGCAAAGTATTTTCTCTTTTA
TCCGCCAAGGGGATAAACAAGAAGATGCGTTAGTTGTCGTATGTAATTTTACGAAAGCTACATATGAAAACTATAAAGTA
GGTGTACCAGATTTCGAGTATTATAACGAGATTTTAAACAGTGATGCTGAGCAATATGGCGGATCAGGGCAAGTAAATAA
GAAACGTCTTAAGACGATTCAAGAACCGTATCATAATCAAACAGCACATGTAGAAATTACAATTCCACCATTTGGCGTAT
CCATATTACGACCAGTGAAGACGAGAAAGGGGAGCAAAAAACAAGATGGCTCAAAAACAAAAGTGCGTAGCAATGTTACT
AGCAGGGGGAAAAGGTAG

Upstream 100 bases:

>100_bases
GAAAAAAACGTATATTTTTTATAAATTAACAAGGATATTTCAGTTTTTCGCAGAATTGGTAAAGTATAAAAAATTCTGTC
ATCTTAGTGAGGTGAGCATA

Downstream 100 bases:

>100_bases
TCGATTAAGTGCATTAACAAAAAATTTAGCCAAGCCAGCTGTTCCATTTGGTGGTAAATATCGCATTATTGATTTTACGC
TAAGTAACTGCGCGAATTCT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSVINCEEVKRDEFHTEKYYESYNIFGAHIVTEDEMRGVRFTVWAPHAKAMSVVGDFNEWDYEQHKMLQVTEEGIWSLFI
PHIEEREIYKYAIETMAGDVIFKADPYAVYAEVRPNTASVVFDIKGYEWNDKNWSRKKKKKSVYKEAMTVYELHFGSWKK
KEDGTLYSYREMAEELIPYVVEHQFTHIEIMPLVEHPYDRSWGYQGTGYYAATSRFGTPYDLMHFVDECHKYGIGVILDW
VPGHFCKDAHGLYLFDGTPTYEYKDKDVQENPVWGTVNFDLGKREVRNFLISNALFWMRYFHIDGFRVDAVANMLYWNKE
GQEQSNEHAVSFLRELNEAVFAEDEDFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHDK
MTFSLLYAYSENFILPLSHDEVVHGKKSLLNKMPGDYWDKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
NLHDFEMHRYMHDYFKELIALYKRSKPLWQLDHSREGFQWIDANNNEQSIFSFIRQGDKQEDALVVVCNFTKATYENYKV
GVPDFEYYNEILNSDAEQYGGSGQVNKKRLKTIQEPYHNQTAHVEITIPPFGVSILRPVKTRKGSKKQDGSKTKVRSNVT
SRGKR

Sequences:

>Translated_645_residues
MSVINCEEVKRDEFHTEKYYESYNIFGAHIVTEDEMRGVRFTVWAPHAKAMSVVGDFNEWDYEQHKMLQVTEEGIWSLFI
PHIEEREIYKYAIETMAGDVIFKADPYAVYAEVRPNTASVVFDIKGYEWNDKNWSRKKKKKSVYKEAMTVYELHFGSWKK
KEDGTLYSYREMAEELIPYVVEHQFTHIEIMPLVEHPYDRSWGYQGTGYYAATSRFGTPYDLMHFVDECHKYGIGVILDW
VPGHFCKDAHGLYLFDGTPTYEYKDKDVQENPVWGTVNFDLGKREVRNFLISNALFWMRYFHIDGFRVDAVANMLYWNKE
GQEQSNEHAVSFLRELNEAVFAEDEDFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHDK
MTFSLLYAYSENFILPLSHDEVVHGKKSLLNKMPGDYWDKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
NLHDFEMHRYMHDYFKELIALYKRSKPLWQLDHSREGFQWIDANNNEQSIFSFIRQGDKQEDALVVVCNFTKATYENYKV
GVPDFEYYNEILNSDAEQYGGSGQVNKKRLKTIQEPYHNQTAHVEITIPPFGVSILRPVKTRKGSKKQDGSKTKVRSNVT
SRGKR
>Mature_644_residues
SVINCEEVKRDEFHTEKYYESYNIFGAHIVTEDEMRGVRFTVWAPHAKAMSVVGDFNEWDYEQHKMLQVTEEGIWSLFIP
HIEEREIYKYAIETMAGDVIFKADPYAVYAEVRPNTASVVFDIKGYEWNDKNWSRKKKKKSVYKEAMTVYELHFGSWKKK
EDGTLYSYREMAEELIPYVVEHQFTHIEIMPLVEHPYDRSWGYQGTGYYAATSRFGTPYDLMHFVDECHKYGIGVILDWV
PGHFCKDAHGLYLFDGTPTYEYKDKDVQENPVWGTVNFDLGKREVRNFLISNALFWMRYFHIDGFRVDAVANMLYWNKEG
QEQSNEHAVSFLRELNEAVFAEDEDFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHDKM
TFSLLYAYSENFILPLSHDEVVHGKKSLLNKMPGDYWDKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDWN
LHDFEMHRYMHDYFKELIALYKRSKPLWQLDHSREGFQWIDANNNEQSIFSFIRQGDKQEDALVVVCNFTKATYENYKVG
VPDFEYYNEILNSDAEQYGGSGQVNKKRLKTIQEPYHNQTAHVEITIPPFGVSILRPVKTRKGSKKQDGSKTKVRSNVTS
RGKR

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=631, Percent_Identity=26.4659270998415, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1789839, Length=608, Percent_Identity=43.0921052631579, Blast_Score=510, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17554896, Length=645, Percent_Identity=25.8914728682171, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI32564391, Length=604, Percent_Identity=25.3311258278146, Blast_Score=161, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6320826, Length=663, Percent_Identity=26.2443438914027, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28573410, Length=678, Percent_Identity=26.6961651917404, Blast_Score=199, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 76105; Mature: 75974

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVINCEEVKRDEFHTEKYYESYNIFGAHIVTEDEMRGVRFTVWAPHAKAMSVVGDFNEW
CCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCEEEEEECCCHHHHHHHCCCCCC
DYEQHKMLQVTEEGIWSLFIPHIEEREIYKYAIETMAGDVIFKADPYAVYAEVRPNTASV
CCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCEEE
VFDIKGYEWNDKNWSRKKKKKSVYKEAMTVYELHFGSWKKKEDGTLYSYREMAEELIPYV
EEEEECEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHH
VEHQFTHIEIMPLVEHPYDRSWGYQGTGYYAATSRFGTPYDLMHFVDECHKYGIGVILDW
HHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEC
VPGHFCKDAHGLYLFDGTPTYEYKDKDVQENPVWGTVNFDLGKREVRNFLISNALFWMRY
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
FHIDGFRVDAVANMLYWNKEGQEQSNEHAVSFLRELNEAVFAEDEDFLMTAEDSTAWPLV
HHCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
TAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHDKMTFSLLYAYSENFILPLSHD
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCEEEECCCC
EVVHGKKSLLNKMPGDYWDKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
NLHDFEMHRYMHDYFKELIALYKRSKPLWQLDHSREGFQWIDANNNEQSIFSFIRQGDKQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHCCCCC
EDALVVVCNFTKATYENYKVGVPDFEYYNEILNSDAEQYGGSGQVNKKRLKTIQEPYHNQ
CCCEEEEEECCHHCCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCC
TAHVEITIPPFGVSILRPVKTRKGSKKQDGSKTKVRSNVTSRGKR
CEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVINCEEVKRDEFHTEKYYESYNIFGAHIVTEDEMRGVRFTVWAPHAKAMSVVGDFNEW
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCEEEEEECCCHHHHHHHCCCCCC
DYEQHKMLQVTEEGIWSLFIPHIEEREIYKYAIETMAGDVIFKADPYAVYAEVRPNTASV
CCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCEEE
VFDIKGYEWNDKNWSRKKKKKSVYKEAMTVYELHFGSWKKKEDGTLYSYREMAEELIPYV
EEEEECEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHH
VEHQFTHIEIMPLVEHPYDRSWGYQGTGYYAATSRFGTPYDLMHFVDECHKYGIGVILDW
HHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEC
VPGHFCKDAHGLYLFDGTPTYEYKDKDVQENPVWGTVNFDLGKREVRNFLISNALFWMRY
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
FHIDGFRVDAVANMLYWNKEGQEQSNEHAVSFLRELNEAVFAEDEDFLMTAEDSTAWPLV
HHCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
TAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHDKMTFSLLYAYSENFILPLSHD
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCEEEECCCC
EVVHGKKSLLNKMPGDYWDKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
NLHDFEMHRYMHDYFKELIALYKRSKPLWQLDHSREGFQWIDANNNEQSIFSFIRQGDKQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHCCCCC
EDALVVVCNFTKATYENYKVGVPDFEYYNEILNSDAEQYGGSGQVNKKRLKTIQEPYHNQ
CCCEEEEEECCHHCCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCC
TAHVEITIPPFGVSILRPVKTRKGSKKQDGSKTKVRSNVTSRGKR
CEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA