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The map label for this gene is adhE [H]

Identifier: 49476825

GI number: 49476825

Start: 4165610

End: 4168213

Strand: Reverse

Name: adhE [H]

Synonym: BT9727_4104

Alternate gene names: 49476825

Gene position: 4168213-4165610 (Counterclockwise)

Preceding gene: 49481445

Following gene: 49478621

Centisome position: 79.58

GC content: 37.86

Gene sequence:

>2604_bases
ATGGTAGTCAAAGAGAAAGTTGTAAATGAAATGCAGGAAGTAAAAGAGATGATTGATACGTTAGTGAATAACGGTCGGAA
GGCTTTACAAGCATTAGAAAGTTTTACACAAGAGCAAATTGACAACATTGTTCATGAAATGGCATTAGCAGGTGTTGATC
AACATATGCCACTTGCGAAATTGGCTGTTGAAGAAACAGGTCGTGGTGTATACGAAGATAAATGCATTAAAAATATTTTT
GCTACTGAATATATTTGGCATAGCATAAAGAAAGATAAAACAGTAGGGATTATTCACGAAGATCCTCATGAAGAAATAAT
AGAAATTGCGGAGCCCGTCGGTGTAGTAGCTGGGGTAACACCAGTAACAAATCCAACATCGACAACAATGTTTAAAGCAT
TAATCGCGATAAAAACGAGAAATCCAATTATTTTCGCATTTCATCCTTCAGCACAAAACTGTTCTATTGCAGCCGCGAAA
ACAGTATACGATGCAGCGGTGAAGGCGGGGGCACCAAAACATTGTGTGCAGTGGATTGAAAAGCCTTCTGTCGAAGCAAC
GAAACAATTAATGAACCATGAAGGTGTTGCGCTTGTTTTGGCAACTGGAGGGGCTGGTATGGTGAAATCAGCTTATTCTA
CTGGAAAACCAGCGCTAGGTGTAGGTCCTGGTAATGTACCATGTTATATAGAGAAATCAGCGCATATAAAACGAGCGGTT
AATGATTTGATTTTATCAAAAACATTTGATAACGGGATGATTTGTGCATCAGAACAAGCAATTATTGTAGATAAGGAAAT
TTATGATGATGTGAAAACAGAAATGATTGCAAATGATTGTTACTTTGTAACAGAGGAAGAGAGAAGAAAATTAGAAAAGC
TTGTTATTAATGAAAATACATGTGCAGTAAATAGTGATATTGTAGGGAAATCCGCACAGTATATTGCCGAATTAGTTGGA
ATTACTGTACCTAAAAATACAAAAATGCTTGTAGCTGAAATACAAGGCATCGGAGCCGCATATCCACTATCACGTGAGAA
GTTAAGTCCAGTATTAGCTTGTGTAAAAGCAAATTCATTAGAAGAAGGATTTACATATTGTGAAGAAATGTTAAACCTCG
GTGGTTTAGGGCATTCAGCAGTTATCCATTCTGTAAATAAAGAAGTGCAAAAGCAATTTGGATTACGTATGAAAGCTTGT
CGCCTTATTGTAAATGCTCCTTCATCACAAGGTGGAATCGGTGACATATATAATGGATTTATTCCATCACTTACGCTTGG
CTGCGGTTCCTACGGGAAAAACTCGGTTTCTCAAAACGTAACAGCGACGCATTTATTAAATATAAAAAGGTTGGCAAATA
GAAAAAAGAATATGCAGTGGTTCAAATTACCACCAAAAATTTATTTTGAAAAACATGCGACAGCATATTTAGCAAATATG
CCTAACATTTCACGTGCGTTTATTGTAACGGATCCAGGAATGGTTGAGCACGGATATGTAGATACGGTCGCTCATTATTT
ACGTAAACATGCAAATGATGTGAAAGTAGAAGTTTTCTTTGAGGTTGAGCCAGATCCATCAGATGAAACTGTGTTTAAAG
GTGCGGAAATGATGAGAAGTTTTCAGCCTGACGTCATTATCGCACTTGGTGGTGGTTCAGCGATGGATGCAGCGAAAGGG
ATGTGGCTTTTCTATGAGCATCCAGAAACAACATTCTATGGAATTAAACAGAAGTTTTTAGATATAAGAAAACGCACATG
TAAATATCCGGAATTAGGAAGTAAGGCGCAGTTTGTTGCCATTCCAACAACATCTGGAACAGGATCAGAAGTGACACCAT
TTGCGGTTATTACAGATAAGAAAAATAATATAAAGTACCCACTCGCGGATTATGAATTAACGCCAGATGTAGCAATTGTA
GATCCGCAATTTGTAATAACAGTACCGCCACATGTAACAGCAGACACTGGGATGGACGTATTAACGCATGCAATCGAGGC
GTATGTGTCTGTTTTAGCAAATGACTATACAGATGGACTAGCATTAAAGGCTATTGATCTCGTATTTAAATATTTACCAA
GAGCATTTAAAGATGGACATGATGAAGAAGCACGAGAAAAAATGCATAATGCTTCTGCAATTGCAGGGATGGCATTTGCA
AATGCATTCCTAGGTATTAATCACAGTTTAGCGCATAAAATTGGACCAGAATTCCATATTCCGCATGGACGTGCGAATGC
CATTCTTATGCCGCACGTAATTCGTTATAATGCTATTAAGCCAAGAAAACATGCATTGTTCCCAAAATATGAGCACTTTG
TGGCTGATGAACGCTATGCACATATTGCGAGAATGCTCGGGCTTCCAGCAAGTTCGGCGGCAGAAGGTGTGGAATCACTC
GTCAAAGCAATTATTGATCTCGGAAAAAGTTTAAATATAAATATGAGTATTGCGGGACAAGGAGTTGCTAAAGACCAATT
TGAAGAGGTTGTTGGATTATTAGCAGAAAGAGCTTTTGAAGATCAATGTACAACAGCTAATCCGAAATTACCTCTCATTT
CAGAGCTGAAAGAAATTTATATGAAAGCATATAAAGGTGAGTAA

Upstream 100 bases:

>100_bases
TCATGTAGGAAAGCAACTCTATTTTTTTTGTGCTAATCGGGACTAATTATGACCCATAGGGTCGTATTTAGTCCAATGTA
GCAGAATAGGAGGAATTTAC

Downstream 100 bases:

>100_bases
TGGTTATGAAAGAGTCGCAGTAAAGCGGCTCTTTTTCTTTTGGAAAACATCTCCCAGAATGGCTGTATTTACTTGTCTTT
AAGTTTAGTAGAATATGATA

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MVVKEKVVNEMQEVKEMIDTLVNNGRKALQALESFTQEQIDNIVHEMALAGVDQHMPLAKLAVEETGRGVYEDKCIKNIF
ATEYIWHSIKKDKTVGIIHEDPHEEIIEIAEPVGVVAGVTPVTNPTSTTMFKALIAIKTRNPIIFAFHPSAQNCSIAAAK
TVYDAAVKAGAPKHCVQWIEKPSVEATKQLMNHEGVALVLATGGAGMVKSAYSTGKPALGVGPGNVPCYIEKSAHIKRAV
NDLILSKTFDNGMICASEQAIIVDKEIYDDVKTEMIANDCYFVTEEERRKLEKLVINENTCAVNSDIVGKSAQYIAELVG
ITVPKNTKMLVAEIQGIGAAYPLSREKLSPVLACVKANSLEEGFTYCEEMLNLGGLGHSAVIHSVNKEVQKQFGLRMKAC
RLIVNAPSSQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVTATHLLNIKRLANRKKNMQWFKLPPKIYFEKHATAYLANM
PNISRAFIVTDPGMVEHGYVDTVAHYLRKHANDVKVEVFFEVEPDPSDETVFKGAEMMRSFQPDVIIALGGGSAMDAAKG
MWLFYEHPETTFYGIKQKFLDIRKRTCKYPELGSKAQFVAIPTTSGTGSEVTPFAVITDKKNNIKYPLADYELTPDVAIV
DPQFVITVPPHVTADTGMDVLTHAIEAYVSVLANDYTDGLALKAIDLVFKYLPRAFKDGHDEEAREKMHNASAIAGMAFA
NAFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNAIKPRKHALFPKYEHFVADERYAHIARMLGLPASSAAEGVESL
VKAIIDLGKSLNINMSIAGQGVAKDQFEEVVGLLAERAFEDQCTTANPKLPLISELKEIYMKAYKGE

Sequences:

>Translated_867_residues
MVVKEKVVNEMQEVKEMIDTLVNNGRKALQALESFTQEQIDNIVHEMALAGVDQHMPLAKLAVEETGRGVYEDKCIKNIF
ATEYIWHSIKKDKTVGIIHEDPHEEIIEIAEPVGVVAGVTPVTNPTSTTMFKALIAIKTRNPIIFAFHPSAQNCSIAAAK
TVYDAAVKAGAPKHCVQWIEKPSVEATKQLMNHEGVALVLATGGAGMVKSAYSTGKPALGVGPGNVPCYIEKSAHIKRAV
NDLILSKTFDNGMICASEQAIIVDKEIYDDVKTEMIANDCYFVTEEERRKLEKLVINENTCAVNSDIVGKSAQYIAELVG
ITVPKNTKMLVAEIQGIGAAYPLSREKLSPVLACVKANSLEEGFTYCEEMLNLGGLGHSAVIHSVNKEVQKQFGLRMKAC
RLIVNAPSSQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVTATHLLNIKRLANRKKNMQWFKLPPKIYFEKHATAYLANM
PNISRAFIVTDPGMVEHGYVDTVAHYLRKHANDVKVEVFFEVEPDPSDETVFKGAEMMRSFQPDVIIALGGGSAMDAAKG
MWLFYEHPETTFYGIKQKFLDIRKRTCKYPELGSKAQFVAIPTTSGTGSEVTPFAVITDKKNNIKYPLADYELTPDVAIV
DPQFVITVPPHVTADTGMDVLTHAIEAYVSVLANDYTDGLALKAIDLVFKYLPRAFKDGHDEEAREKMHNASAIAGMAFA
NAFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNAIKPRKHALFPKYEHFVADERYAHIARMLGLPASSAAEGVESL
VKAIIDLGKSLNINMSIAGQGVAKDQFEEVVGLLAERAFEDQCTTANPKLPLISELKEIYMKAYKGE
>Mature_867_residues
MVVKEKVVNEMQEVKEMIDTLVNNGRKALQALESFTQEQIDNIVHEMALAGVDQHMPLAKLAVEETGRGVYEDKCIKNIF
ATEYIWHSIKKDKTVGIIHEDPHEEIIEIAEPVGVVAGVTPVTNPTSTTMFKALIAIKTRNPIIFAFHPSAQNCSIAAAK
TVYDAAVKAGAPKHCVQWIEKPSVEATKQLMNHEGVALVLATGGAGMVKSAYSTGKPALGVGPGNVPCYIEKSAHIKRAV
NDLILSKTFDNGMICASEQAIIVDKEIYDDVKTEMIANDCYFVTEEERRKLEKLVINENTCAVNSDIVGKSAQYIAELVG
ITVPKNTKMLVAEIQGIGAAYPLSREKLSPVLACVKANSLEEGFTYCEEMLNLGGLGHSAVIHSVNKEVQKQFGLRMKAC
RLIVNAPSSQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVTATHLLNIKRLANRKKNMQWFKLPPKIYFEKHATAYLANM
PNISRAFIVTDPGMVEHGYVDTVAHYLRKHANDVKVEVFFEVEPDPSDETVFKGAEMMRSFQPDVIIALGGGSAMDAAKG
MWLFYEHPETTFYGIKQKFLDIRKRTCKYPELGSKAQFVAIPTTSGTGSEVTPFAVITDKKNNIKYPLADYELTPDVAIV
DPQFVITVPPHVTADTGMDVLTHAIEAYVSVLANDYTDGLALKAIDLVFKYLPRAFKDGHDEEAREKMHNASAIAGMAFA
NAFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNAIKPRKHALFPKYEHFVADERYAHIARMLGLPASSAAEGVESL
VKAIIDLGKSLNINMSIAGQGVAKDQFEEVVGLLAERAFEDQCTTANPKLPLISELKEIYMKAYKGE

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=358, Percent_Identity=26.8156424581006, Blast_Score=99, Evalue=2e-20,
Organism=Escherichia coli, GI1787493, Length=862, Percent_Identity=49.3039443155452, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=387, Percent_Identity=35.1421188630491, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082107, Length=339, Percent_Identity=36.8731563421829, Blast_Score=205, Evalue=1e-53,
Organism=Escherichia coli, GI1789163, Length=382, Percent_Identity=34.0314136125654, Blast_Score=190, Evalue=4e-49,
Organism=Escherichia coli, GI1788797, Length=379, Percent_Identity=30.8707124010554, Blast_Score=160, Evalue=5e-40,
Organism=Escherichia coli, GI1789386, Length=330, Percent_Identity=25.4545454545455, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17537053, Length=251, Percent_Identity=30.2788844621514, Blast_Score=110, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=441, Percent_Identity=34.0136054421769, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24657991, Length=312, Percent_Identity=30.4487179487179, Blast_Score=97, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 95148; Mature: 95148

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKEKVVNEMQEVKEMIDTLVNNGRKALQALESFTQEQIDNIVHEMALAGVDQHMPLAK
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGRGVYEDKCIKNIFATEYIWHSIKKDKTVGIIHEDPHEEIIEIAEPVGVVAGVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
PVTNPTSTTMFKALIAIKTRNPIIFAFHPSAQNCSIAAAKTVYDAAVKAGAPKHCVQWIE
CCCCCCHHHHHHHHHHEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KPSVEATKQLMNHEGVALVLATGGAGMVKSAYSTGKPALGVGPGNVPCYIEKSAHIKRAV
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHH
NDLILSKTFDNGMICASEQAIIVDKEIYDDVKTEMIANDCYFVTEEERRKLEKLVINENT
HHHHHHHCCCCCEEEECCCEEEEEHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCC
CAVNSDIVGKSAQYIAELVGITVPKNTKMLVAEIQGIGAAYPLSREKLSPVLACVKANSL
CEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCH
EEGFTYCEEMLNLGGLGHSAVIHSVNKEVQKQFGLRMKACRLIVNAPSSQGGIGDIYNGF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCHHHHCH
IPSLTLGCGSYGKNSVSQNVTATHLLNIKRLANRKKNMQWFKLPPKIYFEKHATAYLANM
HCHHEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCC
PNISRAFIVTDPGMVEHGYVDTVAHYLRKHANDVKVEVFFEVEPDPSDETVFKGAEMMRS
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
FQPDVIIALGGGSAMDAAKGMWLFYEHPETTFYGIKQKFLDIRKRTCKYPELGSKAQFVA
CCCCEEEEECCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IPTTSGTGSEVTPFAVITDKKNNIKYPLADYELTPDVAIVDPQFVITVPPHVTADTGMDV
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCHHH
LTHAIEAYVSVLANDYTDGLALKAIDLVFKYLPRAFKDGHDEEAREKMHNASAIAGMAFA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
NAFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNAIKPRKHALFPKYEHFVADERYA
HHHHCCCHHHHHHCCCCEECCCCCCCEEECCHHHHCCCCCCHHHCCCCCHHHHHHHHHHH
HIARMLGLPASSAAEGVESLVKAIIDLGKSLNINMSIAGQGVAKDQFEEVVGLLAERAFE
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
DQCTTANPKLPLISELKEIYMKAYKGE
CCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVVKEKVVNEMQEVKEMIDTLVNNGRKALQALESFTQEQIDNIVHEMALAGVDQHMPLAK
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGRGVYEDKCIKNIFATEYIWHSIKKDKTVGIIHEDPHEEIIEIAEPVGVVAGVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
PVTNPTSTTMFKALIAIKTRNPIIFAFHPSAQNCSIAAAKTVYDAAVKAGAPKHCVQWIE
CCCCCCHHHHHHHHHHEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KPSVEATKQLMNHEGVALVLATGGAGMVKSAYSTGKPALGVGPGNVPCYIEKSAHIKRAV
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHH
NDLILSKTFDNGMICASEQAIIVDKEIYDDVKTEMIANDCYFVTEEERRKLEKLVINENT
HHHHHHHCCCCCEEEECCCEEEEEHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCC
CAVNSDIVGKSAQYIAELVGITVPKNTKMLVAEIQGIGAAYPLSREKLSPVLACVKANSL
CEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCH
EEGFTYCEEMLNLGGLGHSAVIHSVNKEVQKQFGLRMKACRLIVNAPSSQGGIGDIYNGF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCHHHHCH
IPSLTLGCGSYGKNSVSQNVTATHLLNIKRLANRKKNMQWFKLPPKIYFEKHATAYLANM
HCHHEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCC
PNISRAFIVTDPGMVEHGYVDTVAHYLRKHANDVKVEVFFEVEPDPSDETVFKGAEMMRS
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
FQPDVIIALGGGSAMDAAKGMWLFYEHPETTFYGIKQKFLDIRKRTCKYPELGSKAQFVA
CCCCEEEEECCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IPTTSGTGSEVTPFAVITDKKNNIKYPLADYELTPDVAIVDPQFVITVPPHVTADTGMDV
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCHHH
LTHAIEAYVSVLANDYTDGLALKAIDLVFKYLPRAFKDGHDEEAREKMHNASAIAGMAFA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
NAFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNAIKPRKHALFPKYEHFVADERYA
HHHHCCCHHHHHHCCCCEECCCCCCCEEECCHHHHCCCCCCHHHCCCCCHHHHHHHHHHH
HIARMLGLPASSAAEGVESLVKAIIDLGKSLNINMSIAGQGVAKDQFEEVVGLLAERAFE
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
DQCTTANPKLPLISELKEIYMKAYKGE
CCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]