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The map label for this gene is xthA2 [H]

Identifier: 49476204

GI number: 49476204

Start: 1794909

End: 1795718

Strand: Reverse

Name: xthA2 [H]

Synonym: BH15490

Alternate gene names: 49476204

Gene position: 1795718-1794909 (Counterclockwise)

Preceding gene: 49476205

Following gene: 49476203

Centisome position: 92.99

GC content: 41.23

Gene sequence:

>810_bases
ATGTTCTTTCGTCTTGCTACTTGGAATATTAACTCTATCCGTTTGCGTCTTGCACAGATTTTTCAGTATTTGGAACTCTT
TTCTGCAGATATTTTATGTTTACAGGAAACGAAATGTCCAGATGCTTTATTTCCAGTTAAGGCTTTTGAAGCGGCCGGAT
ATAAATATATCGCACTCAGTGGACAAAAATCTTACAACGGTGTGGCAATTGTATCACGTTTGCCATTTAAGAGTGTTGAA
AAGCGTTTCTTTTGTCAAAAACAAGAATGTCGTTACATTTCGGTGACTGTTGAAGTTTACGGCAAGAGCATGCGGATTCA
TAATTTTTATGTGCCAGCTGGAGGAGATGTGCCTGACGCTGATGTTAATGAAAAATTTCGCCATAAACTTGATTTTTTAG
AAGAAATGTCTTCCATCCAAGCTGATCAGGGAGATGGTGTTTCTTCTTTTTTGGTAGGCGATTTGAATATTGCTCCTTTG
CCAGAGGATGTTTGGTCTCACAAGCAATTGTTGAAGGTGGTAAGTCATACGCCAATTGAAACCGAGCGGTTACAAGCTTT
ATGCTGCCAAGGTGGGTGGGTTGATCTGATGCGGATGCAGTTTCCTGTTCCTACAAAACTTTATACGTGGTGGAGTTACC
GTGCACGGGACTGGGCACTTGCTGATCGTGGGAGGCGGCTTGATCATATTTGGTCTTCTCCTGATTTAACGTCTTTTGTT
GCAGAGCTTTCAATTTTTCGTCATACACGGGGATGGAATCAGCCTTCAGATCACGTTCCTGTGCAAACTGTATTTGATTT
TTCAGGTTAA

Upstream 100 bases:

>100_bases
AATTATGAATGTGCGTACAGGTGTGCGGTTTGCGGATGGGATGTTTACACTTCCCTCTAAGAAATAAGAATAGGCTATTA
TCTTATCGGAATTAAAACAG

Downstream 100 bases:

>100_bases
TTTTGAAATAAGAAGTAAATAAAATATTAATAGTTGATTCTGGGAAATGCGGGAGACTAGATGCATATCAGAGATCTTAA
AAGGAGTACTTCTAACAGAC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFFRLATWNINSIRLRLAQIFQYLELFSADILCLQETKCPDALFPVKAFEAAGYKYIALSGQKSYNGVAIVSRLPFKSVE
KRFFCQKQECRYISVTVEVYGKSMRIHNFYVPAGGDVPDADVNEKFRHKLDFLEEMSSIQADQGDGVSSFLVGDLNIAPL
PEDVWSHKQLLKVVSHTPIETERLQALCCQGGWVDLMRMQFPVPTKLYTWWSYRARDWALADRGRRLDHIWSSPDLTSFV
AELSIFRHTRGWNQPSDHVPVQTVFDFSG

Sequences:

>Translated_269_residues
MFFRLATWNINSIRLRLAQIFQYLELFSADILCLQETKCPDALFPVKAFEAAGYKYIALSGQKSYNGVAIVSRLPFKSVE
KRFFCQKQECRYISVTVEVYGKSMRIHNFYVPAGGDVPDADVNEKFRHKLDFLEEMSSIQADQGDGVSSFLVGDLNIAPL
PEDVWSHKQLLKVVSHTPIETERLQALCCQGGWVDLMRMQFPVPTKLYTWWSYRARDWALADRGRRLDHIWSSPDLTSFV
AELSIFRHTRGWNQPSDHVPVQTVFDFSG
>Mature_269_residues
MFFRLATWNINSIRLRLAQIFQYLELFSADILCLQETKCPDALFPVKAFEAAGYKYIALSGQKSYNGVAIVSRLPFKSVE
KRFFCQKQECRYISVTVEVYGKSMRIHNFYVPAGGDVPDADVNEKFRHKLDFLEEMSSIQADQGDGVSSFLVGDLNIAPL
PEDVWSHKQLLKVVSHTPIETERLQALCCQGGWVDLMRMQFPVPTKLYTWWSYRARDWALADRGRRLDHIWSSPDLTSFV
AELSIFRHTRGWNQPSDHVPVQTVFDFSG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=27.5735294117647, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=27.8195488721804, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=27.8195488721804, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30972; Mature: 30972

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFRLATWNINSIRLRLAQIFQYLELFSADILCLQETKCPDALFPVKAFEAAGYKYIALS
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCCEEEEEEE
GQKSYNGVAIVSRLPFKSVEKRFFCQKQECRYISVTVEVYGKSMRIHNFYVPAGGDVPDA
CCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEEEEECCEEEEEEEECCCCCCCCCC
DVNEKFRHKLDFLEEMSSIQADQGDGVSSFLVGDLNIAPLPEDVWSHKQLLKVVSHTPIE
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
TERLQALCCQGGWVDLMRMQFPVPTKLYTWWSYRARDWALADRGRRLDHIWSSPDLTSFV
HHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCCCCCHHHHCCCCHHHCCCCCCHHHHH
AELSIFRHTRGWNQPSDHVPVQTVFDFSG
HHHHHHHHHCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MFFRLATWNINSIRLRLAQIFQYLELFSADILCLQETKCPDALFPVKAFEAAGYKYIALS
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCCEEEEEEE
GQKSYNGVAIVSRLPFKSVEKRFFCQKQECRYISVTVEVYGKSMRIHNFYVPAGGDVPDA
CCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEEEEECCEEEEEEEECCCCCCCCCC
DVNEKFRHKLDFLEEMSSIQADQGDGVSSFLVGDLNIAPLPEDVWSHKQLLKVVSHTPIE
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
TERLQALCCQGGWVDLMRMQFPVPTKLYTWWSYRARDWALADRGRRLDHIWSSPDLTSFV
HHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCCCCCHHHHCCCCHHHCCCCCCHHHHH
AELSIFRHTRGWNQPSDHVPVQTVFDFSG
HHHHHHHHHCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]