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The map label for this gene is rpoH2 [H]

Identifier: 49476176

GI number: 49476176

Start: 1746137

End: 1747048

Strand: Reverse

Name: rpoH2 [H]

Synonym: BH15210

Alternate gene names: 49476176

Gene position: 1747048-1746137 (Counterclockwise)

Preceding gene: 49476180

Following gene: 49476174

Centisome position: 90.47

GC content: 41.45

Gene sequence:

>912_bases
ATGGGTAATCTTTCAAATGCTGTTTACAAAAATAGTAAAAATTATAGCGATGCAGACAAAAGTTTGGGACGCAATATGAT
GCGTTCTGCTATGCAGGCGCCTTATCTTGAACGGCAAAAAGAGCATGATTTGGCTCTGCGATGGAAAGACAAACGCGATG
ATGAAGCTATGCATCAAATTGCAGCTGCTCATATGCGTTTGGTGATTGCGATTGCTAACCGTTTTAAACGTTTCAAAATG
CCATTGGGGGATTTGGTACAAGAAGGCTATGTGGGGTTGTTGGAAGCTGCTGCACGTTTTGAACCTGATCGCGAGGTACG
TTTTTCAACTTATGCAACATGGTGGATTCGCGCTTCTATCCAAGATTATATTTTACGCAATTGGTCAATCGTTCGAGGAG
GAACCAGTTCTTCACAAAAGGCACTTTTCTTTAATCTGCGTCGTTTGCGGGCAAAATTGGTGCAAGATGATAGCGCTCTT
TCAAAACAAGATATCTTCCGTAGCATTGCTGAAAAGCTTGGCGTTTCCGTGCGTGATGTTGAAACAATGGATTTCCGTTT
TTCAAGTTCTGATAATTCCTTGAGCGTTTCTGTTTCTGAAAATAGTGATAATCCTGTTGCCAAAATGGATGCTCTTGTGG
ATGATAGTCCTCTACCTGATGCTTTAATTGAACAAGTGATTGATGGTCAAAGGCGCACACAATGGCTTTATGATGCGTTA
CAAATTCTCAATGAACGGGAATTGGAAATTATTCGTTTCCGACGCTTAAATGAAGAAGGTGCTACTTTAGAAGTTTTAGG
TGAAAAGTTAGGAATCTCAAAAGAGCGGGTGCGCCAAATTGAAGCGCGGGCTTTACAAAAGCTGCGTTCTGCCTTGCTTA
CTGTTAATCCAGCAGATGCTTATGATGTGTAA

Upstream 100 bases:

>100_bases
AGTTAGCATAAAAATAAACAACTTTTACGTTCTTTTTTAGAAAGAATGATGATGTCGTTGCCAAATCAACTTTGATAATT
TTTAATCGCAAGGGGTTATT

Downstream 100 bases:

>100_bases
GCCATGAAGTTTTTTCAGGCAGTGGTTCGTGAGTGGTATGGGATAGGGTACAAGAAACTGGATCTCGAAAGCTCGCGCAT
CTGTGTGAAAAAACCTCACC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MGNLSNAVYKNSKNYSDADKSLGRNMMRSAMQAPYLERQKEHDLALRWKDKRDDEAMHQIAAAHMRLVIAIANRFKRFKM
PLGDLVQEGYVGLLEAAARFEPDREVRFSTYATWWIRASIQDYILRNWSIVRGGTSSSQKALFFNLRRLRAKLVQDDSAL
SKQDIFRSIAEKLGVSVRDVETMDFRFSSSDNSLSVSVSENSDNPVAKMDALVDDSPLPDALIEQVIDGQRRTQWLYDAL
QILNERELEIIRFRRLNEEGATLEVLGEKLGISKERVRQIEARALQKLRSALLTVNPADAYDV

Sequences:

>Translated_303_residues
MGNLSNAVYKNSKNYSDADKSLGRNMMRSAMQAPYLERQKEHDLALRWKDKRDDEAMHQIAAAHMRLVIAIANRFKRFKM
PLGDLVQEGYVGLLEAAARFEPDREVRFSTYATWWIRASIQDYILRNWSIVRGGTSSSQKALFFNLRRLRAKLVQDDSAL
SKQDIFRSIAEKLGVSVRDVETMDFRFSSSDNSLSVSVSENSDNPVAKMDALVDDSPLPDALIEQVIDGQRRTQWLYDAL
QILNERELEIIRFRRLNEEGATLEVLGEKLGISKERVRQIEARALQKLRSALLTVNPADAYDV
>Mature_302_residues
GNLSNAVYKNSKNYSDADKSLGRNMMRSAMQAPYLERQKEHDLALRWKDKRDDEAMHQIAAAHMRLVIAIANRFKRFKMP
LGDLVQEGYVGLLEAAARFEPDREVRFSTYATWWIRASIQDYILRNWSIVRGGTSSSQKALFFNLRRLRAKLVQDDSALS
KQDIFRSIAEKLGVSVRDVETMDFRFSSSDNSLSVSVSENSDNPVAKMDALVDDSPLPDALIEQVIDGQRRTQWLYDALQ
ILNERELEIIRFRRLNEEGATLEVLGEKLGISKERVRQIEARALQKLRSALLTVNPADAYDV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=275, Percent_Identity=36, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789098, Length=240, Percent_Identity=34.1666666666667, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=28.2700421940928, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788231, Length=214, Percent_Identity=26.1682242990654, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34688; Mature: 34557

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLSNAVYKNSKNYSDADKSLGRNMMRSAMQAPYLERQKEHDLALRWKDKRDDEAMHQI
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCHHHHHHH
AAAHMRLVIAIANRFKRFKMPLGDLVQEGYVGLLEAAARFEPDREVRFSTYATWWIRASI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
QDYILRNWSIVRGGTSSSQKALFFNLRRLRAKLVQDDSALSKQDIFRSIAEKLGVSVRDV
HHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCC
ETMDFRFSSSDNSLSVSVSENSDNPVAKMDALVDDSPLPDALIEQVIDGQRRTQWLYDAL
EEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHH
QILNERELEIIRFRRLNEEGATLEVLGEKLGISKERVRQIEARALQKLRSALLTVNPADA
HHHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
YDV
CCC
>Mature Secondary Structure 
GNLSNAVYKNSKNYSDADKSLGRNMMRSAMQAPYLERQKEHDLALRWKDKRDDEAMHQI
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCHHHHHHH
AAAHMRLVIAIANRFKRFKMPLGDLVQEGYVGLLEAAARFEPDREVRFSTYATWWIRASI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
QDYILRNWSIVRGGTSSSQKALFFNLRRLRAKLVQDDSALSKQDIFRSIAEKLGVSVRDV
HHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCC
ETMDFRFSSSDNSLSVSVSENSDNPVAKMDALVDDSPLPDALIEQVIDGQRRTQWLYDAL
EEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHH
QILNERELEIIRFRRLNEEGATLEVLGEKLGISKERVRQIEARALQKLRSALLTVNPADA
HHHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
YDV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]