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The map label for this gene is pykA [H]

Identifier: 49476169

GI number: 49476169

Start: 1735591

End: 1737027

Strand: Reverse

Name: pykA [H]

Synonym: BH15120

Alternate gene names: 49476169

Gene position: 1737027-1735591 (Counterclockwise)

Preceding gene: 49476170

Following gene: 49476166

Centisome position: 89.95

GC content: 47.39

Gene sequence:

>1437_bases
GTGAAGCGTGCACGTAAAGTAAAAATTATAGCCACTCTTGGCCCTTCTTCGTTTTCTATTTCCATGATTGAGAAGCTTTT
TATGGCGGGAGCAGATGTTTTTCGTTTGAATATGAGCCATACTGACCGTGAAACAATGGTTGATTTGGTTCAGCGCATAC
GGAAGGTTGAAAAAACTGTTCGCCGGCCAATCGGTATTTTAGTAGATCTCCAGGGACCAAAATTACGCGTCGGCTGTTTT
GATAAAGGGCAAGAAGATTTGCGTATTGGGCAAAGTTTTACCTTGGATGATCGTGATATTCTTGGTGATGCGCAGCGTGT
TTTTTTTCCGCACGGGGAGGTGCTGGCAGCTATTAAACCCGGTGATCGGTTGTTAATTGATGATGGAAAGTTGGAACTGC
TTGCACAGGTGTGTGATGGTCATTCTGTGCAGTGCCTTGTGGTTGCTGGGAACCGTATTTCTGATCGAAAAGGGGTAAGT
CTTCCCGATACAGTTTTGCCTTTTGGGCCAATGACTCTCAAGGATAAAGCCGATCTTCAGGCAGTTTTGGAGCAGCCTGT
TGATTGGGTTGCTCTGTCTTTTATCCAGCGTCCGGAAGATATTATAGCAGTGCGTGCGTTGACCAAAAGCAAAGTGTCTT
TGATGGCTAAGATCGAAAAACCTCAAGCTTTGGAGCATATAGAAGAGATTATTGATGTTGCCGATGGTATTATGATTGCA
CGCGGAGATCTGGGGGTAGAAATGCCCTTGGAGAGGGTTCCTGCCATTCAGATGGAACTTATAAAAGCTTGTCGTTTAGC
TGGAAAGCCCGTCGTGGTGGCCACGCAAATGCTCGAGTCAATGATTACGTCATCTGTTCCTACAAGGGCAGAAGTTTCTG
ATGTTGCGACAGCAGTTTATACGGGAACTGACGCTGTCATGTTGTCTGCGGAATCTGCCTCTGGTCGTTATCCTGAAGAA
GCTGTGCTGATGATGGATCGAATCGCACAACAAATTGAACAAGATCAGACTTATGCGGCTCAAGTTGGCGCACAGCATCC
TGCTCCAGAATCAACGGGGACCGATGCGATTTCTCTCGCTGCGCGTCAGATCGCCGAAACACTGCAATTGACAGCTATCG
TTGCTTATACTGCTTCAGGCACAACGGGTGTGCGCACATCACGGGAGCGTCCAAACAGGCCCATTATTGCCTTATCGCCT
ATTGTAAAAACAGCAAGACGTTTGGCTTTGGTCTGGGGACTTCATTGTGTGGTCACTGAAGACGCGCGGGATTTGGAGGA
TATGGTTGATCGTGCGGCTGCGATTGCTTTTCAAGAAGGCTTTTGCCAAGGGGGAGATCGCTTCCTTGTGACAGCTGGTG
TTCCTCTTGGTACACCTGGTGCTACCAATCTCTTGCGTATCGCTTCTGTTTCTCAAGATGGAACAAAGGGTGTTTAA

Upstream 100 bases:

>100_bases
ATACGGGTAATCAAACCAGTTGGATGGTACAGTTGACCGATGAGTCTTTGTTCAATAATTCCGTTGTACAGTCCCCCTCT
CTTTCAGGAGATTCTCCACC

Downstream 100 bases:

>100_bases
CGGGAACATTGGGGCATAGTTTTCTTTATATTTTGAAGAATATATTCCATTTGTCATCTGTTTTTCTATGAGCAGAGATG
TGTGATGTTTCTGCAGCGGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKRARKVKIIATLGPSSFSISMIEKLFMAGADVFRLNMSHTDRETMVDLVQRIRKVEKTVRRPIGILVDLQGPKLRVGCF
DKGQEDLRIGQSFTLDDRDILGDAQRVFFPHGEVLAAIKPGDRLLIDDGKLELLAQVCDGHSVQCLVVAGNRISDRKGVS
LPDTVLPFGPMTLKDKADLQAVLEQPVDWVALSFIQRPEDIIAVRALTKSKVSLMAKIEKPQALEHIEEIIDVADGIMIA
RGDLGVEMPLERVPAIQMELIKACRLAGKPVVVATQMLESMITSSVPTRAEVSDVATAVYTGTDAVMLSAESASGRYPEE
AVLMMDRIAQQIEQDQTYAAQVGAQHPAPESTGTDAISLAARQIAETLQLTAIVAYTASGTTGVRTSRERPNRPIIALSP
IVKTARRLALVWGLHCVVTEDARDLEDMVDRAAAIAFQEGFCQGGDRFLVTAGVPLGTPGATNLLRIASVSQDGTKGV

Sequences:

>Translated_478_residues
MKRARKVKIIATLGPSSFSISMIEKLFMAGADVFRLNMSHTDRETMVDLVQRIRKVEKTVRRPIGILVDLQGPKLRVGCF
DKGQEDLRIGQSFTLDDRDILGDAQRVFFPHGEVLAAIKPGDRLLIDDGKLELLAQVCDGHSVQCLVVAGNRISDRKGVS
LPDTVLPFGPMTLKDKADLQAVLEQPVDWVALSFIQRPEDIIAVRALTKSKVSLMAKIEKPQALEHIEEIIDVADGIMIA
RGDLGVEMPLERVPAIQMELIKACRLAGKPVVVATQMLESMITSSVPTRAEVSDVATAVYTGTDAVMLSAESASGRYPEE
AVLMMDRIAQQIEQDQTYAAQVGAQHPAPESTGTDAISLAARQIAETLQLTAIVAYTASGTTGVRTSRERPNRPIIALSP
IVKTARRLALVWGLHCVVTEDARDLEDMVDRAAAIAFQEGFCQGGDRFLVTAGVPLGTPGATNLLRIASVSQDGTKGV
>Mature_478_residues
MKRARKVKIIATLGPSSFSISMIEKLFMAGADVFRLNMSHTDRETMVDLVQRIRKVEKTVRRPIGILVDLQGPKLRVGCF
DKGQEDLRIGQSFTLDDRDILGDAQRVFFPHGEVLAAIKPGDRLLIDDGKLELLAQVCDGHSVQCLVVAGNRISDRKGVS
LPDTVLPFGPMTLKDKADLQAVLEQPVDWVALSFIQRPEDIIAVRALTKSKVSLMAKIEKPQALEHIEEIIDVADGIMIA
RGDLGVEMPLERVPAIQMELIKACRLAGKPVVVATQMLESMITSSVPTRAEVSDVATAVYTGTDAVMLSAESASGRYPEE
AVLMMDRIAQQIEQDQTYAAQVGAQHPAPESTGTDAISLAARQIAETLQLTAIVAYTASGTTGVRTSRERPNRPIIALSP
IVKTARRLALVWGLHCVVTEDARDLEDMVDRAAAIAFQEGFCQGGDRFLVTAGVPLGTPGATNLLRIASVSQDGTKGV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=34.5528455284553, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=34.5528455284553, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=33.8085539714868, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=32.7902240325866, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=32.7902240325866, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=31.1418685121107, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=31.1418685121107, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=27.0042194092827, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=27.0042194092827, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=25.5813953488372, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787965, Length=462, Percent_Identity=35.2813852813853, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=37.2141372141372, Blast_Score=265, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=34.4398340248963, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=35.1851851851852, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=35.1851851851852, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=35.1851851851852, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=35.1851851851852, Blast_Score=244, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=34.826883910387, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=33.4020618556701, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=33.8842975206612, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI28571814, Length=484, Percent_Identity=33.8842975206612, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=34.3137254901961, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=30.2040816326531, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24646914, Length=256, Percent_Identity=36.71875, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51750; Mature: 51750

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRARKVKIIATLGPSSFSISMIEKLFMAGADVFRLNMSHTDRETMVDLVQRIRKVEKTV
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RRPIGILVDLQGPKLRVGCFDKGQEDLRIGQSFTLDDRDILGDAQRVFFPHGEVLAAIKP
CCCCEEEEECCCCEEEEECCCCCHHHHHCCCCCCCCCHHHHCCCCEEECCCCCEEEEECC
GDRLLIDDGKLELLAQVCDGHSVQCLVVAGNRISDRKGVSLPDTVLPFGPMTLKDKADLQ
CCEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
AVLEQPVDWVALSFIQRPEDIIAVRALTKSKVSLMAKIEKPQALEHIEEIIDVADGIMIA
HHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEE
RGDLGVEMPLERVPAIQMELIKACRLAGKPVVVATQMLESMITSSVPTRAEVSDVATAVY
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHE
TGTDAVMLSAESASGRYPEEAVLMMDRIAQQIEQDQTYAAQVGAQHPAPESTGTDAISLA
ECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ARQIAETLQLTAIVAYTASGTTGVRTSRERPNRPIIALSPIVKTARRLALVWGLHCVVTE
HHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHEEEEEEC
DARDLEDMVDRAAAIAFQEGFCQGGDRFLVTAGVPLGTPGATNLLRIASVSQDGTKGV
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHEEEEEECCCCCCCCC
>Mature Secondary Structure
MKRARKVKIIATLGPSSFSISMIEKLFMAGADVFRLNMSHTDRETMVDLVQRIRKVEKTV
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RRPIGILVDLQGPKLRVGCFDKGQEDLRIGQSFTLDDRDILGDAQRVFFPHGEVLAAIKP
CCCCEEEEECCCCEEEEECCCCCHHHHHCCCCCCCCCHHHHCCCCEEECCCCCEEEEECC
GDRLLIDDGKLELLAQVCDGHSVQCLVVAGNRISDRKGVSLPDTVLPFGPMTLKDKADLQ
CCEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
AVLEQPVDWVALSFIQRPEDIIAVRALTKSKVSLMAKIEKPQALEHIEEIIDVADGIMIA
HHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEE
RGDLGVEMPLERVPAIQMELIKACRLAGKPVVVATQMLESMITSSVPTRAEVSDVATAVY
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHE
TGTDAVMLSAESASGRYPEEAVLMMDRIAQQIEQDQTYAAQVGAQHPAPESTGTDAISLA
ECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ARQIAETLQLTAIVAYTASGTTGVRTSRERPNRPIIALSPIVKTARRLALVWGLHCVVTE
HHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHEEEEEEC
DARDLEDMVDRAAAIAFQEGFCQGGDRFLVTAGVPLGTPGATNLLRIASVSQDGTKGV
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA